“…The difference between CGF and LPCGF is in the type of test tubes used. Solid CGF uses glass red cap tubes (PV 200R-Medifuge Blood Separator CGF P Cycle), while liquid LPCGF uses the red cap tubes (PV 200R) with added sodium heparin or blue cap tubes (PV 200P) with separator gel and sodium citrate (Medifuge Blood Separator CGF Cycle) [18][19][20].…”
Section: Sacco's Protocol For Obtaining Concentrated Growth Factors I...mentioning
Platelet-rich fibrin (PRF) as a biological scaffold is attracting clinicians’ attention, mainly because it promotes bone and soft tissue healing. As autologous material, PRF has many advantages over other platelet concentrates, such as Platelet-rich plasma (PRP) and Plasma rich in growth factors (PRGF). Among many benefits, simple preparation (centrifugation protocol) stands out because no additional anticoagulant is added to the tubes. This chapter aims to clarify the PRF membranes and sticky bone preparation together with other platelet concentrates. A few clinical cases will show how sticky bone is together with PRF membranes applicative in different oral surgery indications. Clinical and radiological check-ups demonstrated excellent therapeutic outcomes. Sticky bone and PRF membranes have regenerative potential and are advised to use in many oral surgery procedures.
“…The difference between CGF and LPCGF is in the type of test tubes used. Solid CGF uses glass red cap tubes (PV 200R-Medifuge Blood Separator CGF P Cycle), while liquid LPCGF uses the red cap tubes (PV 200R) with added sodium heparin or blue cap tubes (PV 200P) with separator gel and sodium citrate (Medifuge Blood Separator CGF Cycle) [18][19][20].…”
Section: Sacco's Protocol For Obtaining Concentrated Growth Factors I...mentioning
Platelet-rich fibrin (PRF) as a biological scaffold is attracting clinicians’ attention, mainly because it promotes bone and soft tissue healing. As autologous material, PRF has many advantages over other platelet concentrates, such as Platelet-rich plasma (PRP) and Plasma rich in growth factors (PRGF). Among many benefits, simple preparation (centrifugation protocol) stands out because no additional anticoagulant is added to the tubes. This chapter aims to clarify the PRF membranes and sticky bone preparation together with other platelet concentrates. A few clinical cases will show how sticky bone is together with PRF membranes applicative in different oral surgery indications. Clinical and radiological check-ups demonstrated excellent therapeutic outcomes. Sticky bone and PRF membranes have regenerative potential and are advised to use in many oral surgery procedures.
“…This fosters an environment conducive to bone regeneration. Additionally, with its unique flowability and injectability, LPCGF can be integrated with other tissue engineering materials. − This highlights its remarkable biocompatibility and carrier capabilities. Essentially, the distinctive composition and biological characteristics of LPCGF make it a formidable candidate for a bioscaffold in bone tissue engineering.…”
The application of bioengineering techniques for achieving bone regeneration in the oral environment is an increasingly prominent field. However, the clinical use of synthetic materials carries certain risks. The liquid phase of concentrated growth factor (LPCGF), as a biologically derived material, exhibits superior biocompatibility. In this study, LPCGF was employed as a tissue engineering scaffold, hosting dental follicle cells (DFCs) to facilitate bone regeneration. Both in vivo and in vitro experimental results demonstrate that this platform significantly enhances the expression of osteogenic markers in DFCs, such as alkaline phosphatase (ALP), runt-related transcription factor 2 (Runx2), and type I collagen (Col1a1). Simultaneously, it reduces the expression of inflammation-related genes, particularly interleukin-6 (IL-6) and interleukin-8 (IL-8), thereby alleviating the negative impact of the inflammatory microenvironment on DFCs. Further investigation into potential mechanisms reveals that this process is regulated over time by the WNT pathway. Our research results demonstrate that LPCGF, with its favorable physical characteristics, holds great potential as a scaffold. It can effectively carry DFCs, thereby providing an optimal initial environment for bone regeneration. Furthermore, LPCGF endeavors to closely mimic the mechanisms of bone healing post-trauma to facilitate bone formation. This offers new perspectives and insights into bone regeneration engineering.
“…Therefore, autologous adipose tissue, as an ideal soft tissue repair material, has been widely used in various causes of soft tissue volume loss or contour deformation 1 . However, the volume retention rate after autologous fat transplantation is unstable, unpredictable, 2 and highly absorbent, often resulting in poor postoperative results or the need for repeated operations 3,4 . Among them, the survival of transplanted adipose tissue is the key factor to determine the postoperative volume retention rate.…”
Autologous adipose tissue transplantation is widely used for cosmetic and reconstruction of various areas in the body, often to repair soft tissue volume loss or contoured deformation. However, the application of fat transplantation is limited by unstable and unpredictable volume retention rates. At present, promoting adipose tissue survival and inhibiting its death is the key to improve the effect of autologous fat transplantation. In this paper, we propose a hypothesis that ferroptosis exists in fat transplantation. The bases of this hypothesis include the following: (1) the association between ferroptosis and other programmed cell death; (2) the association between ferroptosis and ischemia-reperfusion injury; and (3) the use of ferroptosis inhibitors in the field of fat transplantation.
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