Liquid optical phantoms mimicking spectral characteristics of laboratory mouse biotissues

Loginova, Daria; Сергеева, Е. А.; Krainov, A D; Агрба, П. Д.; Kirillin, Mikhail

doi:10.1070/qel16133

Cited by 19 publications

(17 citation statements)

References 6 publications

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“…Many groups have attempted to address this need through the addition of more specific absorbers with the inclusion of red ink being a relatively popular choice as it provides higher absorption in the green and blue regions of the spectrum while maintaining a low absorption in the red and NIR. 19 Cosmetic powders matched to different skin-tones have also been used to provide similar spectral absorption differentials, though are less commonly used due to their comparatively high scattering. 20 Different molecular dyes with relatively narrow absorption peaks have also been used in order to independently tune two separate wavelengths to the desired properties.…”

Section: Introductionmentioning

confidence: 99%

Development of a platform for broadband, spectra-fitted, tissue optical phantoms

Walter

Jansen

2023

J. Biomed. Opt.

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Significance: Current methods of producing optical phantoms are incapable of accurately capturing the wavelength-dependent properties of tissue critical for many optical modalities. Aim:We aim to introduce a method of producing solid, inorganic phantoms whose wavelengthdependent optical properties can be matched to those of tissue over the wavelength range of 370 to 950 nm.Approach: The concentration-dependent optical properties of 20 pigments were characterized and used to determine combinations that result in optimal fits compared to the target properties over the full spectrum. Phantoms matching the optical properties of muscle and nerve, the diffuse reflectance of pale and melanistic skin, and the chromophore concentrations of a computational skin model with varying oxygen saturation (StO 2 ) were made with this method.Results: Both optical property phantoms were found to accurately mimic their respective tissues' absorption and scattering properties across the entire spectrum. The diffuse reflectance phantoms were able to closely approximate skin reflectance regardless of skin type. All three computational skin phantoms were found to have emulated chromophore concentrations close to the model, with an average percent error for the StO 2 of 4.31%.Conclusions: This multipigment phantom platform represents a powerful tool for creating spectrally accurate tissue phantoms, which should increase the availability of standards for many optical techniques.

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Section: Introductionmentioning

confidence: 99%

Development of a platform for broadband, spectra-fitted, tissue optical phantoms

Walter

Jansen

2023

J. Biomed. Opt.

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“…For Monte Carlo simulations in phantom the optical properties of 2% aqueous solution of Intralipid [29] were reconstructed from spectrophotometry measurements with Analytik Jena Specord 250Plus system (Germany). The obtained values at the wavelength of 532 nm are μ s = 5.37 mm −1 , μ a = 0.057 mm −1 , g = 0.631.…”

Section: Methodsmentioning

confidence: 99%

Fluence compensation in raster-scan optoacoustic angiography

et al. 2017

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Modern optical imaging techniques demonstrate significant potential for high resolution in vivo angiography. Optoacoustic angiography benefits from higher imaging depth as compared to pure optical modalities. However, strong attenuation of optoacoustic signal with depth provides serious challenges for adequate 3D vessel net mapping, and proper compensation for fluence distribution within biotissues is required. We report on the novel approach allowing to estimate effective in-depth fluence profiles for optoacoustic systems. Calculations are based on Monte Carlo simulation of light transport and account for complex illumination geometry and acoustic detection parameters. The developed fluence compensation algorithm was tested in in vivo angiography of human palm and allowed to overcome significant in-depth attenuation of probing radiation and enhance the contrast of lower dermis plexus while preserving high resolution of upper plexus imaging.

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“…Figure 3 shows the photos of the phantom filled with water ( Figure 4a) and with agar mixture (Figure 4b). Preparation of tissue mimicking phantoms, their characterization, and comparison with real biotissues are described in details in previous papers [8,25,26]. Optical properties of the employed agar mixture and their comparison to the values for murine brain ex vivo are summarized in Table 1.…”

Section: Phantom Designmentioning

confidence: 99%

Towards Bimodal Optical Monitoring of Photodynamic Therapy with Targeted Nanoconstructs: A Phantom Study

et al. 2019

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Increase of the efficiency of photodynamic therapy (PDT) requires the development of advanced protocols employing both novel photosensitizer (PS) carriers and aids for online monitoring. Nanoconstructs may be comprised of a photosensitizer, chemotherapy drugs, or inhibitors of molecular pathways that support cancer growth. In this paper, we analyze the efficiency of a bimodal approach involving fluorescence and optoacoustic imaging in monitoring drug distribution and photobleaching. The study evaluates typical sensitivities of these techniques to the presence of the two key moieties of a nanoconstruct: benzoporphyrin derivatives (BPD) serving as a PS, and IRDye800 acting as a contrast agent. Both imaging modalities employ dual-wavelength probing at the wavelengths corresponding to absorption peaks of BPD and IRDye800, which enables their separate detection. In an experiment on a tissue-mimicking phantom with inclusions containing separate BPD and IRDye800 solutions, fluorescence imaging demonstrated higher contrast as compared to optoacoustic imaging for both components, though strong light scattering in the surrounding media restricted accurate localization of the markers. It was also sensitive to photobleaching, which is a measure of PDT efficiency. Optoacoustic imaging demonstrated sufficient sensitivity to both components, though less than that of fluorescence imaging, however, it enabled depth-resolved detection of an absorber and estimation of its relative content. Employment of the bimodal approach in monitoring of PS photobleaching adds to its potential in intraprocedural PDT monitoring.

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Liquid optical phantoms mimicking spectral characteristics of laboratory mouse biotissues

Cited by 19 publications

References 6 publications

Development of a platform for broadband, spectra-fitted, tissue optical phantoms

Development of a platform for broadband, spectra-fitted, tissue optical phantoms

Fluence compensation in raster-scan optoacoustic angiography

Towards Bimodal Optical Monitoring of Photodynamic Therapy with Targeted Nanoconstructs: A Phantom Study

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