Liquid modification of Baird-Parker's medium for the selective enrichment of Staphylococcus aureus

Abstract

A liquid modification of Baird-Parker's medium is suggested for the detection of low (less than 20/g) numbers of Staphylococcus aureus. Model experiments showed that the medium had an acceptable level of selectivity and that it was non-inhibitory to injured cells. Practical evaluation demonstrated the advantage of selective enrichment procedures over both non-selective enrichment technique and direct plating methods.

Isolation of Methicillin-Resistant Staphylococcus aureus (MRSA) from Mares with Metritis and its Zooepidemiology

“…Isolation of MRSA: For isolation of MRSA, telluriteglycine-pyruvate broth [7] supplemented with 50 µg/ml gentamicin was used as a selective enrichment medium and egg yolk-tellurite-glycine-pyruvate agar [2] supplemented with and without 50 µg/ml gentamicin as selective media. After thawed, each specimen was centrifuged at 3,000 rpm for 10 min and the deposit was divided into three equal parts and inoculated onto the above-described three types of culture media.…”

Staphylococcus aureus and related staphylococci in foods: ecology, proliferation, toxinogenesis, control and monitoring

“…Unlike the situation with the detection of salmonellas in foods, where a universally applicable method has yet to be devised (Beckers et al 1987;DAoust 1989;Perales & Audicana 1989), there is a strong body of opinion that the medium of Baird-Parker (1962) provides an excellent basis for the detection and enumeration of Staph. aureus and related staphylococci in foods (de Waart et al 1968;Rayman et al 1978Rayman et al , 1988Idziak & Mossel 1980;van Doorne et al 1981van Doorne et al , 1982Bouwer-Hertzberger et al 1982;Mossel et al 1985;van Netten et al 1990). Some slight modifications have been necessary, but we believe that there is no reason to continue the search for different media, while attachment to media that were inferior to begin with (White et al 1988) should be discouraged.…”

Staphylococci in milk and milk products

“…Recovery is improved by the addition of sodium pyruvate(Lancette et al 1986) or Tween 80 (Anon. 1989 Van Doorne et al (1981). suggested a liquid modification of Baird-Parker's medium, (LBP) for selective enrichment of Staph.…”