Liquid–Liquid Phase Separation Sheds New Light upon Cardiovascular Diseases

Cai, Ziyang; Mei, Shuai; Zhou, Li; Ma, Xiaozhu; Wuyun, Qidamugai; Yan, Jiangtao; Ding, Hu

doi:10.3390/ijms242015418

Cited by 2 publications

(1 citation statement)

References 137 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…38 This process leads to the formation of BMCs, which are dynamic, membraneless compartments in cells that have significant roles in cellular functions. 39 A specific kind of BMCs, known as membrane-less organelles, display characteristics similar to organelles but lack a membrane boundary. 40 In the context of cellular biology, these terms are interconnected yet unique, with LLPS being the process and BMCs and membrane-less organelles being the resulting structures.…”

Section: Molecular and Cellular Aspects Of Protein And Rna Condensationmentioning

confidence: 99%

Intrinsic factors behind long COVID: IV. Hypothetical roles of the SARS‐CoV‐2 nucleocapsid protein and its liquid–liquid phase separation

Eltayeb,

Al‐Sarraj,

Alharbi

et al. 2024

J of Cellular Biochemistry

View full text Add to dashboard Cite

When the SARS‐CoV‐2 virus infects humans, it leads to a condition called COVID‐19 that has a wide spectrum of clinical manifestations, from no symptoms to acute respiratory distress syndrome. The virus initiates damage by attaching to the ACE‐2 protein on the surface of endothelial cells that line the blood vessels and using these cells as hosts for replication. Reactive oxygen species levels are increased during viral replication, which leads to oxidative stress. About three‐fifths (~60%) of the people who get infected with the virus eradicate it from their body after 28 days and recover their normal activity. However, a large fraction (~40%) of the people who are infected with the virus suffer from various symptoms (anosmia and/or ageusia, fatigue, cough, myalgia, cognitive impairment, insomnia, dyspnea, and tachycardia) beyond 12 weeks and are diagnosed with a syndrome called long COVID. Long‐term clinical studies in a group of people who contracted SARS‐CoV‐2 have been contrasted with a noninfected matched group of people. A subset of infected people can be distinguished by a set of cytokine markers to have persistent, low‐grade inflammation and often self‐report two or more bothersome symptoms. No medication can alleviate their symptoms efficiently. Coronavirus nucleocapsid proteins have been investigated extensively as potential drug targets due to their key roles in virus replication, among which is their ability to bind their respective genomic RNAs for incorporation into emerging virions. This review highlights basic studies of the nucleocapsid protein and its ability to undergo liquid–liquid phase separation. We hypothesize that this ability of the nucleocapsid protein for phase separation may contribute to long COVID. This hypothesis unlocks new investigation angles and could potentially open novel avenues for a better understanding of long COVID and treating this condition.

show abstract

Section: Molecular and Cellular Aspects Of Protein And Rna Condensationmentioning

confidence: 99%

Intrinsic factors behind long COVID: IV. Hypothetical roles of the SARS‐CoV‐2 nucleocapsid protein and its liquid–liquid phase separation

Eltayeb,

Al‐Sarraj,

Alharbi

et al. 2024

J of Cellular Biochemistry

View full text Add to dashboard Cite

show abstract

Modified-liquid-liquid phase separation: A novel technique to synthesize 2-dimensional CuO nanosheets

Mahajan,

Saroja,

Sundara

et al. 2024

Preprint

View full text Add to dashboard Cite

The 2-dimensional materials are seeing an upsurge in research and various applications. In this work, the growth of cupric oxide (CuO) nanostructures in different morphologies is reported by employing modified-liquid-liquid phase separation (m-LLPS) technique. Here, two binary phases copper sulphate-polyethylene glycol (PEG) i.e. C&P and sodium hydroxide-PEG i.e. N&P are used in tandem and reduction of copper salt is employed. Sheet-like agglomerated growth could be restrained into nice flattened disks by merely modifying the interaction of binary phases in time. X-ray diffraction (XRD) for structural information, UV-Visible spectroscopy for optical bandgap estimation, micro-Raman spectroscopy for vibrational levels investigation, and Fourier Transform Infrared spectroscopy (FTIR) as fingerprint for bond/ compound formation have been employed. The flat-disk CuO sample was used for detection of glucose. Limit of detection and limit of quantification levels were estimated and were found to be 4 mM and 13 mM, respectively. The mechanism of the modified-LLPS method leading to oriented growth from disoriented growth is discussed. By taking CuO as an example, a very simple concept of phase separation has been demonstrated to prepare 2-dimensional materials.

show abstract

Liquid–Liquid Phase Separation Sheds New Light upon Cardiovascular Diseases

Cited by 2 publications

References 137 publications

Intrinsic factors behind long COVID: IV. Hypothetical roles of the SARS‐CoV‐2 nucleocapsid protein and its liquid–liquid phase separation

Intrinsic factors behind long COVID: IV. Hypothetical roles of the SARS‐CoV‐2 nucleocapsid protein and its liquid–liquid phase separation

Modified-liquid-liquid phase separation: A novel technique to synthesize 2-dimensional CuO nanosheets

Contact Info

Product

Resources

About