Liquid–liquid phase separation of proteins and peptides derived from biological materials: Discovery, protein engineering, and emerging applications

Sun, Yue; Lim, Zhi Wei; Guo, Qi; Yu, Jing; Miserez, Ali

doi:10.1557/mrs.2020.301

Cited by 26 publications

(27 citation statements)

References 97 publications

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“…In some cases, coacervates consist of multiple components that are immiscible leading to coexisting multiphase LLPS [14,16]. Protein coacervates have been reviewed recently in detail [13][14][15]17].…”

Section: Condensed Fluid Phasesmentioning

confidence: 99%

From vesicles to materials: bioinspired strategies for fabricating hierarchically structured soft matter

Amstad

Harrington

2021

Phil. Trans. R. Soc. A.

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Certain organisms including species of mollusks, polychaetes, onychophorans and arthropods produce exceptional polymeric materials outside their bodies under ambient conditions using concentrated fluid protein precursors. While much is understood about the structure-function relationships that define the properties of such materials, comparatively less is understood about how such materials are fabricated and specifically, how their defining hierarchical structures are achieved via bottom-up assembly. Yet this information holds great potential for inspiring sustainable manufacture of advanced polymeric materials with controlled multi-scale structure. In the present perspective, we first examine recent work elucidating the formation of the tough adhesive fibres of the mussel byssus via secretion of vesicles filled with condensed liquid protein phases (coacervates and liquid crystals)—highlighting which design principles are relevant for bio-inspiration. In the second part of the perspective, we examine the potential of recent advances in drops and additive manufacturing as a bioinspired platform for mimicking such processes to produce hierarchically structured materials. This article is part of the theme issue ‘Bio-derived and bioinspired sustainable advanced materials for emerging technologies (part 1)’.

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Section: Condensed Fluid Phasesmentioning

confidence: 99%

From vesicles to materials: bioinspired strategies for fabricating hierarchically structured soft matter

Amstad

Harrington

2021

Phil. Trans. R. Soc. A.

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“…Second, our earlier reports demonstrate that to use these proteins as a binding matrix for biocomposites, it is crucial to initially induce their phase separation and condensation into highly concentrated assemblies, often described as coacervates (Figure 2e,f and Figures S2-S6, Supporting Information). [27][28][29][30] Key advantages of concentrated coacervates include increased intermolecular interactions, low viscosity and interfacial tension, all of which greatly facilitate wetting and infiltration through the CNC network to result in strong binding to the reinforcing phase. This allowed the coacervate microdroplets to mediate the binding of CNC rods, which could not be possible in the absence of coacervation (Figure 2i).…”

Section: Selection Of Building-block Componentsmentioning

confidence: 99%

Bioinspired Functionally Graded Composite Assembled Using Cellulose Nanocrystals and Genetically Engineered Proteins with Controlled Biomineralization

et al. 2021

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Nature provides unique insights into design strategies evolved by living organisms to construct robust materials with a combination of mechanical properties that are challenging to replicate synthetically. Hereby, inspired by the impact‐resistant dactyl club of the stomatopod, a mineralized biocomposite is rationally designed and produced in the complex shapes of dental implant crowns exhibiting high strength, stiffness, and fracture toughness. This material consists of an expanded helicoidal organization of cellulose nanocrystals (CNCs) mixed with genetically engineered proteins that regulate both binding to CNCs and in situ growth of reinforcing apatite crystals. Critically, the structural properties emerge from controlled self‐assembly across multiple length scales regulated by rational engineering and phase separation of the protein components. This work replicates multiscale biomanufacturing of a model biological material and also offers an innovative platform to synthesize multifunctional biocomposites whose properties can be finely regulated by colloidal self‐assembly and engineering of its constitutive protein building blocks.

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“…Sun et al provide a review of recent developments in understanding how liquid-liquid phase separation is used by organisms to assemble proteinbased materials and how this understanding can be leveraged in engineered systems. 57 In native organisms, many structural proteins are found in nanocomposites alongside inorganic materials (e.g., bone and nacre). In such systems, the protein not only serves as the matrix material, but also serves to guide the assembly or formation of the inorganic reinforcement.…”

Section: In This Issuementioning

confidence: 99%

Engineered proteins as multifunctional materials

2020

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Liquid–liquid phase separation of proteins and peptides derived from biological materials: Discovery, protein engineering, and emerging applications

Abstract: Abstract

Cited by 26 publications

References 97 publications

From vesicles to materials: bioinspired strategies for fabricating hierarchically structured soft matter

From vesicles to materials: bioinspired strategies for fabricating hierarchically structured soft matter

Bioinspired Functionally Graded Composite Assembled Using Cellulose Nanocrystals and Genetically Engineered Proteins with Controlled Biomineralization

Engineered proteins as multifunctional materials

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