Liquid–liquid extraction of bromelain and polyphenol oxidase using aqueous two-phase system

Babu, B. Ravindra; Rastogi, N. K.; Raghavarao, K.S.M.S.

doi:10.1016/j.cep.2007.08.006

Cited by 174 publications

(107 citation statements)

References 16 publications

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“…Researchers have reported the used ATPS for extraction and purification of bromelain from stem and peel of pineapple (Ferreira et al 2014). Extraction and purification of bromelain complexed with polyphenol oxidase through ATPS from the pineapple resulted in 228% active recovery with 4 fold rise in purification (Babu et al 2008). ATPS has been used to recover bromelain from the peel of pineapple and gave 113.54% and 206% active recovery with 2.23 and 3.44 purification fold (Ketnawa et al 2010).…”

Section: Aqueous Two Phase System (Atps)mentioning

confidence: 99%

“…Several chromatographic techniques such as ion exchange chromatography (Hung et al 2002;Arumugam and Ponnusami 2013;Iara et al 2013;Swaroop and Viswanathan 2013), high-speed counter-current chromatography (HSCCC) (Yin et al 2011), affinity chromatography, gel filtration chromatography and capillary electrochromatography (Cheng and Huang 2004;Babu et al 2008;Chen et al 2008) have been employed for the purification of bromelain. All these techniques possess different specifications thus result in different purification efficiency and recovery (Yin et al 2011).…”

Section: Chromatographic Techniquesmentioning

confidence: 99%

“…It is also a potential postoperatively agent that assists healing and decrease post surgical discomfort and swelling (Graf 2000). Besides clinical approach, bromelain has also employed in various industries including food industries (Maurer 2001), such as breweries (Soares et al 2012) and flesh processing and tenderization, textile and cosmetic industries (Babu et al 2008;Ketnawal et al 2009). With the advent of recombinant DNA technology, scientists and researchers across the globe have been working on recombinant bromelain to achieve exaggerate and novel applications in future (Arshad et al 2014).…”

Section: Introductionmentioning

confidence: 99%

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Bromelain: Methods of Extraction, Purification and Therapeutic Applications

Manzoor

Nawaz

Mukhtar

et al. 2016

Braz. arch. biol. technol.

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Section: Aqueous Two Phase System (Atps)mentioning

confidence: 99%

Section: Chromatographic Techniquesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Bromelain: Methods of Extraction, Purification and Therapeutic Applications

Manzoor

Nawaz

Mukhtar

et al. 2016

Braz. arch. biol. technol.

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“…The latex was thawed and mixed with 0.1 molar (M) phosphate buffer (pH 7) on a 1:1.5 weight/volume ratio following [10]. The mixture was well agitated for homogeneity before being centrifuged at 9 000 x g for 30 minutes at 4ᵒC to remove solids.…”

Section: Preparation Of Crude Latex Solutionmentioning

confidence: 99%

Application of an Aqueous Two-Phase Technique for Extraction of Papain from Carica Papaya Latex

2017

IJACEBS

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Abstract-An aqueous two phase system made up of polyethylene glycol (PEG) 3350 and potassium citrate was used to extract papain from papaya latex. The assessment of system parameters: PEG 3350, potassium citrate and sodium chloride concentrations on papain purification were studied. The enzyme partitioned preferentially to the bottom phase in all the experiments. Increasing sodium chloride up to 5% increased purification of papain. The highest purification (12 fold) was achieved using potassium citrate 20%-PEG14% at pH 7 and 4 ºC. This study showed that an environmentally friendly extraction system for papain can be developed and further optimized.

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“…Some minor cysteine endopeptidases (ananain, comosain) are also present in the enzyme from pineapple stem. The enzyme extracted from the stem is called stem Bromelain (EC 3.4.22.32) and from fruit is called fruit Bromelain (EC 3.4.22.33) (Babu et al, 2008). It is made up of 212 amino acids and the molecular weight is 33 kDa (Heinicke and Gortner, 1957;Murachi and Neuratii, 1960).…”

Section: Introductionmentioning

confidence: 99%

Optimisation of Bromelain Enzyme Extraction from Pineapple (<i>Ananas comosus</i>) and Application in Process Industry

Mohan

Sivakumar

Rangasamy

et al. 2016

American Journal of Biochemistry and Biotechnology

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Bromelain is a general name for a family of sulfhydryl proteolytic enzymes obtained from Ananas comosus, the pineapple plant. The objective of the present study is to compare the activity of Bromelain from pineapple fruit and peel and to check the efficiency of this crude Bromelain enzyme in unhairing step in leather processing. The crude Bromelain was extracted and activity of enzyme was measured using tyrosine as standard and expressed in Units/mL of enzyme. The effect of preservatives such as sodium benzoate and sodium metabisulphite on the activity of the enzyme was studied for one week. The effect of pH (3-5) was studied using suitable pH buffer solutions. The kinetics of the proteolytic activity of the enzyme was studied using casein as substrate. It was found that the optimum pH of the crude extract was 4.5. In general enzyme activity got affected by the use of preservatives. The kinetic studies show that the activity of enzyme was increasing during initial period. The unhairing activity of crude extract of pH 4.5 was also studied using cow hide pieces. After 24 h treatment with the extracted crude Bromelain enzyme, good unhairing efficacy was achieved for leather making.

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Liquid–liquid extraction of bromelain and polyphenol oxidase using aqueous two-phase system

Cited by 174 publications

References 16 publications

Bromelain: Methods of Extraction, Purification and Therapeutic Applications

Bromelain: Methods of Extraction, Purification and Therapeutic Applications

Application of an Aqueous Two-Phase Technique for Extraction of Papain from Carica Papaya Latex

Optimisation of Bromelain Enzyme Extraction from Pineapple (<i>Ananas comosus</i>) and Application in Process Industry

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