Liquid filling in standard circular well microplates

Abstract

It is often assumed that droplets dispensed into standard microplate wells will automatically fill their bottoms. We show here by computational simulation and experimental verification that the ability to fill the well bottom is dependent on the surface wetting characteristics. The release of droplets at the center was also found to fill the well bottom better than droplet dispensation in contact with the well wall. Hydrophobic surfaces required higher liquid volumes to fill the well bottom; unlike the case with capillary wells microplates. This renders standard microplate wells less amenable for use in small volume liquid handling; a feature that is increasingly sought after in screening and studies involving scarce agents.

Increased Stability and Size of a Bubble on a Superhydrophobic Surface

“…Surface Evolver software was used to compute the predicted profile and size of inverted droplets. , It does this using an energy-minimization method that evolves a surface down the energy gradient. The assumption made in the computation was that an inverted static water drop on a surface should be identical to an immersed bubble on another surface with an opposing contact angle (as described in Figure ).…”

Using the Meniscus in a Capillary for Small Volume Contact Angle Measurement in Biochemical Applications

“…This method would be difficult to apply on small liquid volumes as it has been recently shown that it has a possibility of not filling the well bottom of standard microplates completely. 16 We show here that the shape of the meniscus in a circular capillary itself reveals the required information to determine the CA. Capillaries have been found to possess useful liquid filling characteristics that render them beneficial for use as alternative types of microplates with improved features.…”

Pengembangan Biosensor Berbasis Plastik Zona Mikro untuk Skrining Aktivitas Antidiabetes pada Ekstrak Tanaman Obat

“…Jika dibandingkan dengan microplate, biosensor ini memiliki beberapa keunggulan diantaranya harganya yang lebih terjangkau, pemakaiannya yang sederhana, dan analisisnya mudah bila menggunakan scanner karena bentuknya yang tipis. Menurut Lu dkk penggunaan microplate standar dengan diameter 6,5 mm, diperlukan volume cairan setidaknya 80 μL untuk menutup seluruh permukaan dasar microplate sedangkan pada penelitian ini menggunakan volume akhir yang lebih sedikit yaitu 20 μL [16]. Biosensor ini juga dapat digunakan secara berulang dengan mencuci biosensor kemudian mengganti lapisan alas dengan lapisan plastik yang baru.…”