Liquid culture system for shoot multiplication and secoiridoid production in micropropagated plants of Centaurium erythraea Rafn

The influence of liquid systems for shoot multiplication, secondary metabolite production and plant regeneration of Scutellaria alpina

“…The lowest rooting percentage was obtained for S. alpina shoots from the liquid medium supported with polyurethane foam; 72% of the shoots developed roots after 4 weeks of culture (Table 1). Centaurium erythraea shoots from liquid culture demonstrated a similar decrease in root formation (Piątczak et al 2005). However, in that case, decreased shoot length was observed in the liquid medium.…”

Mist trickling bioreactor for Centaurium erythraea Rafn growth of shoots and production of secoiridoids

“…This is the first report of shoot culture of C. erythraea in a bioreactor. Earlier, we reported that shake-flask system could be used for C. erythraea shoot multiplication and secoiridoid production (Piatczak et al 2005). In bioreactor culture, a 6-times higher yield of secoiridoids was achieved in less time (from 4 to 3 weeks).…”

“…The secoiridoid content (gentiopicroside, sweroside and swertiamarin as well as the sum of the glucosides) were analyzed in methanol extracts of dried shoots. They were separated, identified and quantified by HPLC, as described earlier (Piatczak et al 2005).…”

In VitroCloning of Date PalmPhoenix DactyliferaL., Cv. Deglet Bey by Using Embryogenic Suspension and Temporary Immersion Bioreactor (TIB)

“…It is known that the hyperhydricity phenomenon is major problem for regeneration of diverse species cultured in liquid or in agar-solidified media (29). Kevers et al (18) reported that hyperhydration is due to a deficient lignification caused by a serious reduction of peroxydases and phenylalanine ammoniaclyase activities.…”