Liquid chromatography–tandem mass spectrometry to determine the stability of collagen pentapeptide (KTTKS) in rat skin

Park, Eun Ji; Kim, Myung Sun; Choi, Yun Lim; Shin, Yooleemi; Lee, Hye Suk; Na, Dong Hee

doi:10.1016/j.jchromb.2012.08.010

Cited by 7 publications

(7 citation statements)

References 13 publications

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“…The inter-day precision results for cysteamine and cystamine were 0.35–2.71% and 0.36–3.95%, respectively. Accuracy was defined as the mean percentage of analyte recovered in the assay (19). The intra-day accuracies for cysteamine and cystamine were 100.6–107.2% and 97.6–102.0%, respectively.…”

Section: Resultsmentioning

confidence: 99%

Simultaneous Determination of Cysteamine and Cystamine in Cosmetics by Ion-Pairing Reversed-Phase High-Performance Liquid Chromatography

Kim

2019

ToxicolRes

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Cysteamine has been used in cosmetics as an antioxidant, a hair straightening agent, and a hair waving agent. However, recent studies indicate that cysteamine can act as an allergen to hairdressers. The objective of this study was to develop and validate a simple and effective reversed-phase high-performance liquid chromatography (RP-HPLC) method for the measurement of cysteamine and its dimer, cystamine. Sodium 1-heptanesulfonate (NaH-pSO) was used as an ion-pairing agent to improve chromatographic performance. Separation was performed on a Gemini C18 column (250 mm × 4.6 mm, 5 µm particle size) using a mobile phase composed of 85:15 (v/v) 4 mM NaHpSO in 0.1% phosphoric acid:acetonitrile. UV absorbance was monitored at 215 nm. The RP-HPLC method developed in this study was validated for specificity, linearity, limit of detection, limit of quantitation, precision, accuracy, and recovery. Cysteamine and cystamine were chromatographically resolved from other reducing agents such as thioglycolic acid and cysteine. Extraction using water and chloroform resulted in the recovery for cysteamine and cystamine ranging from 100.2-102.7% and 90.6-98.7%, respectively. This validated RP-HPLC method would be useful for quality control and monitoring of cysteamine and cystamine in cosmetics.

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Section: Resultsmentioning

confidence: 99%

Simultaneous Determination of Cysteamine and Cystamine in Cosmetics by Ion-Pairing Reversed-Phase High-Performance Liquid Chromatography

Kim

2019

ToxicolRes

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“…The retention times of pal-KTTKS and IS were 1.6 and 3.5 min, respectively. LC-MS/MS of KTTKS was performed using the previously developed methods ( Park et al ., 2012 ).…”

Section: Resultsmentioning

confidence: 99%

“…Recently, we reported the development of a LC-MS/MS me thod for monitoring the stability of KTTKS in rat skin ( Park et al ., 2012 ). In this study, a LC-MS/MS method was developed for the quantification of pal-KTTKS via simultaneous mo nitoring of the selected MS/MS transitions for pal-KTTKS and pal-AA, the IS.…”

Section: Discussionmentioning

confidence: 99%

“…LC-MS/MS was performed using an Agilent Series 1200 LC instrument with 6410 Triple Quad LC-MS/MS system (Agilent Technologies, Palo Alto, CA, USA). Analysis of KTTKS was performed using Acclaim 300 C 18 column (2.1×150 mm, 3 μm, Dionex, Sunnyvale, CA, USA) by isocratic elution using a mobile phase consisting of 5 mM PFPA aqueous solution and acetonitrile (87:13, v/v) as described previously ( Park et al ., 2012 ). Pal-KTTKS was analyzed using Capcell Pak C8 column (2.0×100 mm, 5 μm, Shisheido, Tokyo, Japan) by isocratic elution using a mobile phase consisting of 20 mM ammonium acetate solution and acetonitrile (30:70, v/v) containing 0.05% acetic acid.…”

Section: Methodsmentioning

confidence: 99%

“…For these studies, appropriate analytical methods should be also developed. Recently, we reported a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to monitor the stability of KTTKS in rat skin ( Park et al ., 2012 ). Chirita et al .…”

Section: Introductionmentioning

confidence: 99%

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Dermal Stability and In Vitro Skin Permeation of Collagen Pentapeptides (KTTKS and palmitoyl-KTTKS)

Choi

Park

Kim

et al. 2014

Biomolecules & Therapeutics

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Collagen pentapeptide (Lys-Thr-Thr-Lys-Ser, KTTKS) and its palmitoylated derivative (pal-KTTKS) have received a great deal of attention as cosmeceutical ingredients for their anti-wrinkle effects. The objective of this study was to evaluate stability and permeability of KTTKS and pal-KTTKS in hairless mouse skin. In this study, a liquid chromatography-tandem mass spectrometric method was developed for the quantification of pal-KTTKS, and used for stability and permeability studies. Stability studies were performed using skin extracts and homogenates. Both KTTKS and pal-KTTKS were rapidly degraded, but pal-KTTKS was more stable than KTTKS. When protease inhibitors were added, the stability of both compounds (KTTKS and pal-KTTKS) improved significantly. In the skin permeation study, neither KTTKS nor pal-KTTKS was detected in the receptor solution, which indicates that neither compound could permeate through the full-thickness hairless mouse skin in the experimental conditions of this study. While KTTKS was not detected in any of the skin layers (the stratum corneum, epidermis, and dermis), pal-KTTKS was observed in all skin layers: 4.2 ± 0.7 μg/cm2 in the stratum corneum, 2.8 ± 0.5 μg/cm2 in the epidermis, and 0.3 ± 0.1 μg/cm2 in the dermis. In conclusion, this study indicated that pal-KTTKS had greater stability and permeability than that of un-modified KTTKS, and may be a useful anti-wrinkle and anti-aging cosmeceutical agent.

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Microneedle-mediated delivery of cosmeceutically relevant nucleoside and peptides in human skin: challenges and strategies for dermal delivery

Lee

2019

J. Pharm. Investig.

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Liquid chromatography–tandem mass spectrometry to determine the stability of collagen pentapeptide (KTTKS) in rat skin

Cited by 7 publications

References 13 publications

Simultaneous Determination of Cysteamine and Cystamine in Cosmetics by Ion-Pairing Reversed-Phase High-Performance Liquid Chromatography

Simultaneous Determination of Cysteamine and Cystamine in Cosmetics by Ion-Pairing Reversed-Phase High-Performance Liquid Chromatography

Dermal Stability and In Vitro Skin Permeation of Collagen Pentapeptides (KTTKS and palmitoyl-KTTKS)

Microneedle-mediated delivery of cosmeceutically relevant nucleoside and peptides in human skin: challenges and strategies for dermal delivery

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