Liquid Chromatography–Tandem Mass Spectrometry Multiclass Method for 46 Antibiotics Residues in Milk and Meat: Development and Validation

Jank, Louíse; Martins, Magda Targa; Arsand, Juliana Bazzan; Motta, Tanara Magalhães Campos; Feijó, Tiago Corrêa; Castilhos, Tamara dos Santos; Hoff, Rodrigo Barcellos; Barreto, Fabiano; Pizzolato, Tânia Mara

doi:10.1007/s12161-016-0755-4

Cited by 51 publications

(20 citation statements)

References 35 publications

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“…Three milk samples from each cow were collected and tested for the presence of antibiotics residues at intervals of 0, 24, 48, 72, and 96 h respectively after drugs administration. All samples were analyzed by the kit in present study as described in the “Evaluation Protocol” section and by a multi-residue LC/MS-MS method ( Jank et al, 2017 ).…”

Section: Methodsmentioning

confidence: 99%

A Novel Microbiological Method in Microtiter Plates for Screening Seven Kinds of Widely Used Antibiotics Residues in Milk, Chicken Egg and Honey

Peng

Liu

et al. 2019

Front. Microbiol.

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A broad-spectrum microbiological inhibition method has been developed for rapidly screening different kinds of antibiotics such as β-lactam, aminoglycosides, tetracyclines, sulfonamides, macrolides, lincosamides and quinolones in milk, chicken egg and honey by using an easy sample preparation. The microbiological system in microtiter plates consists of an agar medium, a mixture of nutrients, test bacteria (Geobacillus stearothermophilus var C953), bromocresol purple, and other supplements such as trimethoprim, chloramphenicol, streptomycin and enrofloxacin which helps to improve the detection capability of the microbiological system toward the chosen antibiotics. It was observed that the limit of detection of the kit used in present study for all kinds of antibiotics in milk were lower than or close to maximum residue limits determined by EU or CODEX. For chicken egg and honey, the detection capability of the kit was similar to that determined in milk. Moreover, it was revealed that the kit in present study was more sensitive to aminoglycosides, macrolides and quinolones in various matrixes than internationally available commercial kits. The false-positive and false-negative rates for both were 0%. The coefficient of variations among various factors was all less than 4%. Additionally, the quality guarantee period of the kit was more than 6 months at 4°C. A good correlation between the kit results and the LC–MS/MS results for milk was also observed, which revealed that the kit was reliable to screen antibiotics residues in incurred samples.

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Section: Methodsmentioning

confidence: 99%

A Novel Microbiological Method in Microtiter Plates for Screening Seven Kinds of Widely Used Antibiotics Residues in Milk, Chicken Egg and Honey

Peng

Liu

et al. 2019

Front. Microbiol.

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“…As a result, several methods have been reported to measure the two antibiotics and their metabolites in pharmaceutical preparations, including using UPLC-photodiode array detector (PAD) [8], high-performance liquid chromatography (HPLC)-ultraviolet (UV) detector [9,10,11], HPLC-fluorescence detector (FLD) [12], reverse-phase (RP)-HPLC-FLD [13], LC-MS/MS [5,14,15,16,17,18,19] and UPLC-MS/MS [20,21,22] analyses. In an earlier study, we reported on an RP-HPLC–FLD method [13] and an LC-MS/MS method [18] for the simultaneous analysis and confirmation of the two antibiotics and their metabolite residues in eggs.…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of an HPLC-ESI/MS/MS Method for the Determination of Amoxicillin, Its Major Metabolites, and Ampicillin Residues in Chicken Tissues

et al. 2019

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A method for the simultaneous analysis of amoxicillin (AMO), amoxicillin metabolites, and ampicillin residues in edible chicken muscle, liver, and kidney samples via high-performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI/MS/MS) was developed and verified. The extraction and purification procedures involved the extraction of the sample using a liquid-liquid extraction method with acetonitrile to eliminate the proteins. The chicken tissue extract was then injected directly onto an HPLC column coupled to a mass spectrometer with an ESI(+) source. The HPLC-ESI/MS/MS method was validated according to specificity, sensitivity, linearity, matrix effects, precision, accuracy, decision limit, detection capability, and stability, as defined by the European Union and Food and Drug Administration. The linearity was desirable, and the determination coefficients (r2 values) ranged from 0.9968 and 0.9999. The limits of detection and limits of quantification were 0.10–2.20 μg/kg and 0.30–8.50 μg/kg, respectively. The decision limits were 57.71–61.25 μg/kg, and the detection capabilities were 65.41–72.50 μg/kg, and the recoveries of the four target analytes exceeded 75% at the limits of quantification and exceeded 83% at 25, 50, and 100 μg/kg (n = 6 at each level), confirming the reliability of this method for determining these analytes and providing a new detection technology. For real sample analysis, this experiment tested 30 chicken tissue samples, only one chicken muscle, liver, and kidney sample were contaminated with 5.20, 17.45, and 7.33 μg/kg of AMO values, respectively, while other target compounds were not detected in the 30 tested chicken tissue samples.

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“…HPLC contains characteristics like a variety of mobile phases, extensive library of column packings and variation in modes of operations. [12] The probability of potential adverse health effects caused by antimicrobial residue can be measure by calculating the risk assessment. Generally, chemical risk assessment consist of four well-defined steps-hazard identification, hazard characterization/dose-response assessment, exposure assessment and risk characterization [13].…”

Section: Introductionmentioning

confidence: 99%

<strong>Determination and Human Health Risk Assessment of Antibiotic Residue in Cow Milk in Bangladesh</strong>

Rahman¹,

Hassan²,

Chowdhury³

2020

Preprint

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Consumption of milk contaminated with antibiotic residues above the maximum residue limit (MRL) causes direct toxicity to humans and the development of superbugs that leads to the failure of antibiotic therapy and threatens human life. Besides, long-duration exposure might alter the nature of gut microflora results in the enhancement of many diseases. Therefore, we examined 300 raw and processed packet milk samples using thin layer chromatography (TLC) and ultra high performance liquid chromatography (UHPLC) method against five veterinary antibiotics and assessed the risk for consumers in Chattogram, Bangladesh. Risk analysis was calculated by using hazard quotient on the basis of 165 ml per capita milk consumption. We found a total of 7% prevalence of antibiotic residues in raw milk samples which were higher (8%) in individual samples than the pooled samples (4%). However, we did not find any processed packet milk samples as positive. The mean concentration of oxytetracycline residue was detected 61.29 µg/l and amoxicillin was 124 µg/l in individual milk samples. Risk analysis showed the hazard quotient values of 0.0056 for oxytetracycline and 0.0017 for amoxicillin residue which was confirmed that, no significant health risk associated with the consumption of milk produced and marketed in the study area. Our study might fill the gap of knowledge to measure the safety status of milk regarding public health issues.

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Liquid Chromatography–Tandem Mass Spectrometry Multiclass Method for 46 Antibiotics Residues in Milk and Meat: Development and Validation

Cited by 51 publications

References 35 publications

A Novel Microbiological Method in Microtiter Plates for Screening Seven Kinds of Widely Used Antibiotics Residues in Milk, Chicken Egg and Honey

A Novel Microbiological Method in Microtiter Plates for Screening Seven Kinds of Widely Used Antibiotics Residues in Milk, Chicken Egg and Honey

Development and Validation of an HPLC-ESI/MS/MS Method for the Determination of Amoxicillin, Its Major Metabolites, and Ampicillin Residues in Chicken Tissues

<strong>Determination and Human Health Risk Assessment of Antibiotic Residue in Cow Milk in Bangladesh</strong>

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