Liquid Chromatography–Tandem Mass Spectrometry Method for the Determination of Meloxicam and its Metabolite 5-Carboxymeloxicam in Human Plasma

Abstract

This method has been successfully applied to the pharmacokinetic study of meloxicam and 5-carboxymeloxicam after oral administration of meloxicam (15 mg) to 30 volunteers.

Determination of meloxicam in human plasma by ultra‐high‐performance liquid chromatography–tandem mass spectrometry and its application in a pharmacokinetic study

“…Several high‐performance liquid chromatography–tandem mass spectrometry (LC–MS/MS) methods for the determination of meloxicam in human plasma have been reported. However, these methods are limited by insufficient stability (Wiesner et al, 2003), large sample volume, long run time (Rigato et al, 2006; Yuan et al, 2007), expensive sample preparation for solid‐phase extraction (Shirako et al, 2013) or time‐consuming and arduous liquid–liquid extraction sample preparation (Ji et al, 2005; Lee et al, 2009). Consequently, the methods do not meet the requirements for high‐throughput in biosample analysis.…”

Microanalysis of Two Members of Oxicam Drugs by Quenching the Fluorescence of Newly Isolated Carbonaceous Materials From Incense Ash

“…The B.P described an HPLC method for assaying both TEN and MEL in their dosage forms [4], also the USP described another HPLC method for assaying MEL in its dosage forms [5]. Numerous other analytical techniques have been described for determination of the two cited drugs including ; spectrophotometric methods [6,7], spectro uorimetric methods [6,8], chromatographic methods [6,[9][10][11][12][13][14] and electrochemical methods [6,[15][16][17]. Despite these techniques are sensitive and accurate, they are also suffering from some shortages, such as using expensive instruments, tedious operation procedures and time consuming.…”

Quantitative determination of meloxicam in dog plasma by high performance liquid chromatography–tandem mass spectrometry and its application in a pharmacokinetic study

“…HPLC with UV/DAD (Bae, Kim, Jang, & Lee, ; Dasandi, Shivaprakash, Saroj, & Bhat, ; Liew, Loh, Tan, & Peh, ) had a sensitivity of about 50 ng/mL, but these methods needed a long chromatography run time (>12 min) and a large volume biological sample (>0.25 mL). High‐performance liquid chromatography–tandem mass spectrometry (HPLC‐MS/MS) was used in the determination of meloxicam (Ji, Lee, Kim, Jeong, & Lee, ; Lee, Ji, Kim, Lee, & Lee, ; Shirako et al, ). However its application was limited by the arduous and expensive sample preparation by solid‐phase extraction (Shirako et al, ) or time‐consuming and complicated sample preparation by liquid–liquid extraction (Ji et al, ; Lee et al, ), which could not meet the requirement for high throughput and speed in biosample analysis.…”

“…High‐performance liquid chromatography–tandem mass spectrometry (HPLC‐MS/MS) was used in the determination of meloxicam (Ji, Lee, Kim, Jeong, & Lee, ; Lee, Ji, Kim, Lee, & Lee, ; Shirako et al, ). However its application was limited by the arduous and expensive sample preparation by solid‐phase extraction (Shirako et al, ) or time‐consuming and complicated sample preparation by liquid–liquid extraction (Ji et al, ; Lee et al, ), which could not meet the requirement for high throughput and speed in biosample analysis. Yuan, Chen, and Zhong () reported an HPLC‐MS/MS method using rapid and simple sample preparation with protein precipitation (PPT) that had good sensitivity with a lower limit of quantification (LLOQ) of 0.10 ng/mL.…”