Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) Method for the Quantification of Steroids Androstenedione, Dehydroepiandrosterone, 11-Deoxycortisol, 17-Hydroxyprogesterone, and Testosterone

Munar, Ada; Frazee, C. Clinton; Garg, Uttam

doi:10.1007/978-1-0716-2565-1_40

Cited by 1 publication

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“…В последнее время для исследования стероидного профиля в плазме используется метод высокоэффективной жидкостной хроматографии и тандемной масс-спектрометрии (ВЭЖХ МС/МС), так как, в отличие от ИФА, ВЭЖХ МС/МС позволяет исследовать одновременно концентрации нескольких надпочечниковых стероидов с высокой точностью [11][12][13][14]. Помимо этого, при интерпретации полученных результатов необходимо учитывать тот факт, что забор крови является «стрессорным» фактором для ребенка и может приводить к повышению уровней исследуемых стероидов [15].…”

Section: Introductionunclassified

A promising approach for therapy control in congenital adrenal hyperplasia. Problems of Endocrinology

Tiulpakov,

Nagaeva,

Kalinchenko

et al. 2024

Probl Endokrinol (Mosk)

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Congenital adrenal hyperplasia (CAH) is a group of autosomal recessive disorders requiring lifelong glucocorticoid replacement (GC) therapy. Lack of GC therapy leads to precocious puberty in boys, heterosexual development in girls, accelerated bone maturation and short final height in both sexes. In adolescence, the lack of GC therapy is the cause of menstrual disorders in girls and the development of TART in boys, as a result reducing the reproductive potential in both sexes. On the other hand, an overdose of GC leads to drug-induced Itsenko-Cushing’s syndrome. In order to select adequate doses of GC in childhood and adolescence, multiple determinations of 17-hydroxyprogesterone, androstenedione, and testosterone in blood plasma, and thus multiple venous blood sampling are required. The blood sampling requires specially trained medical staff and can effect on the results due to stress reaction especially in young patients. Hence, the development and implementation of a non-invasive method for determining the steroid profile is extremely important in monitoring GC therapy in children. In addition, the currently used immunofluorescence assay cannot determine other adrenal steroids, has a high variation due to the «cross-reaction» of steroids that are similar in structure, which inflates the results. Unlike immunofluorescence assay, liquid chromatography and tandem mass spectrometry is more preferable method, since it is more specific and accurate. In this literature review, saliva presented as an alternative substrate and the non-invasive method for determining the steroid profile. This method can solve the above disadvantages, simplify and make more accurate the selection of GC therapy in patients with CAH, which is especially important in childhood.

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