Liquid chromatography–tandem mass spectrometry for clinical diagnostics

Abstract

Mass spectrometry is a powerful analytical tool used for the analysis of a wide range of substances and matrices; it is increasingly utilized for clinical applications in laboratory medicine. This Primer includes an overview of basic mass spectrometry concepts, focusing primarily on tandem mass spectrometry. We discuss experimental considerations and quality management, and provide an overview of some key applications in the clinic. Lastly, the Primer discusses significant challenges for implementation of mass spectrometry in clinical laboratories and provides an outlook of where there are emerging clinical applications for this technology.

Integrating a Multiple Isotopologue Reaction-Monitoring Technique and LC-MS/MS for Quantitation of Small Molecules: Ten Mycotoxins in Cereals as an Example

“…High-quality commercial standards of ten mycotoxins (chemical purity >98%) were utilized in this study, such as AFB 1 , AFB 2 , AFG 1 , AFG 2 , OTA, T-2, HT-2, DON, ZEN, and FB 1 . Additionally, stable isotopically labeled internal standards were employed, namely, 13 C 17 -AFB 1 , 13 C 17 -AFB 2 , 13 C 17 -AFG 1 , 13 C 17 -AFG 2 , 13 C 20 -OTA, 13 C 24 -T-2, 13 C 22 −HT-2, 13 C 15 -DON, 13 C 18 -ZEN, and 13 C 34 -FB 1 . These standards and internal standards were all procured from Pribolab (Qingdao, China).…”

“…11,12 Since mass spectrometry lacks inherent calibration, diverse calibration strategies have been developed to enhance accuracy and precision. 13 Consequently, in the pursuit of a dependable analytical approach, the creation of calibration curves stands out as a pivotal phase. 14−16 The present state of research methods predominantly concentrated on individual mycotoxins or specific mycotoxin types, employing the conventional multisample external calibration curve (MSCC).…”

Quantitative Profiling Method for Oxylipins in Neurodegenerative Diseases by Liquid Chromatography Coupled with Tandem Mass Spectrometry

“…While the longer dwell time provides a higher number of measurements across a peak, experiencing higher noise is inevitable. 27 Thus, to get the maximum number of measurements with minimum noise, the acquisition was divided into ten functions based on the retention time of the compounds. We employed the "auto" feature available in the MassLynx V4.2 software, which automatically determined the dwell time by considering the number of transitions in each function.…”

Bioinspired Fluorine Labeling for 19F NMR-Based Plasma Amine Profiling

“…Amino acids and biogenic amines play crucial roles in various physiological processes. Understanding their metabolic pathways and regulation through amine metabolite profiling is essential for uncovering the underlying mechanisms of diseases and disorders, including neurological and metabolic disorders, and cancer. − Moreover, amine metabolite profiling holds significant implications in drug discovery, toxicology, and personalized medicine. , The current techniques for amine profiling heavily rely on mass spectrometry (MS) in combination with chromatographic methods, such as liquid chromatography (LC) or gas chromatography (GC). − Although MS is highly sensitive and capable of detecting a wide range of metabolites in complex mixtures, quantifying metabolites remains a challenge due to intrinsic matrix effects and ion suppression …”