Liquid chromatography–tandem mass spectrometry based method development and validation of S016-1271 (LR8P), a novel cationic antimicrobial peptide for its application to pharmacokinetic studies

Short peptides based on the conserved regions of MIEN1 protein exhibit anticancer activity by targeting the MIEN1 signaling pathway

“…The D-isomers and scrambled analogs, namely LA3IK SCR and RP-7 SCR, served two key purposes: D-isomers displayed improved metabolic stability and anticancer activity, while scrambled analogs with swapped amino acid positions highlighted the significance of the third leucine and the prenylation motif CVIL in their anticancer activity. For the bioanalysis, LC/MS methods were preferred over immunological approaches due to time and cost considerations ( 49 ). Pharmacokinetic assays corroborated the improved stability and enhanced pharmacokinetic parameters of D-LA3IK and D-RP-7.…”

Applications of tandem mass spectrometry (MS/MS) in antimicrobial peptides field: Current state and new applications

“…To achieve a specific detection concentration and purity of the target substance, pretreatment conditions should be explored before applying each quantification method [ 188 , 189 ]. Second, the detection range of MS is limit despite its high sensitivity and specificity [ [171] , [190] , [191] ]. After establishing the methodology, the performance of the linear detection range was verified.…”

Shaping the Future of Antimicrobial Therapy: Harnessing the Power of Antimicrobial Peptides in Biomedical Applications

“…For example, protease inhibitors, metal chelators, or antioxidants can be added to protect AMPs from degradation by enzymes or reactive oxygen species [147]. Optimization of pharmacokinetic properties and the method validation of peptides can also help to prevent AMP-coating failures and degradation in clinical applications [148]. Materials with properties such as high surface area, stability, and compatibility should be used in coating applications.…”