Liquid chromatography–tandem mass spectrometric assay for the light sensitive calcium channel antagonist lacidipine in human plasma

Karra, Vijaya Kumari; Pilli, Nageswara Rao; Polagani, Srinivasa Rao; Inamadugu, Jaswanth Kumar; Rao, J. V. L. N. Seshagiri

doi:10.1016/j.jpba.2012.02.022

Cited by 5 publications

(2 citation statements)

References 16 publications

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“…1. As earlier publications have discussed the details of fragmentation patterns of carbamazepine 16 we do not present the data pertaining to this. The LC-MRM technique was chosen for the assay development due its inherent selectivity and sensitivity.…”

Section: Methods Developmentmentioning

confidence: 96%

Bioanalysis of riluzole in human plasma by a sensitive LC-MS/MS method and its application to a pharmacokinetic study in South Indian subjects

et al. 2014

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Section: Methods Developmentmentioning

confidence: 96%

Bioanalysis of riluzole in human plasma by a sensitive LC-MS/MS method and its application to a pharmacokinetic study in South Indian subjects

et al. 2014

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“…The active trans form is used in therapy (32)(33)(34)(35)(36) . Some analytical methods for the determination of lacidipine alone or/and combination with other drug in pharmaceutical dosage form and biological fluids have been reported, including some spectrophotometric method (37,38) , HPLC (39)(40)(41)(42)(43) , Stability-Indicating LC Method (44,45) high performance thin layer chromatography (HPTLC) (46) , LC-MS/MS methods (47)(48)(49)(50) , ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method (51) and SFC-MS/MS (52) . Monotherapy with various antihypertensive agents is not always sufficient to control the blood pressure and concomitant use of two or more drugs is necessary in 50% of the hypertensive patients (19) .…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Estimation of Metoprolol Succinate and Lacidipine in Binary Combination Using High Performance Liquid Chromatographic Method = تقييم في وقت واحد للجمع بين ثنائي السكسينات ميتوبرولول و لاسيدبين باستخدام طريقة الكروماتوجرافي السائلة عالية الأداء

Jain¹,

Jain²,

Jain³

2016

JJPS

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A simple, reliable, rapid, precise, sensitive, rugged and validated RP-HPLC method has been developed to determine Metoprolol succinate and Lacidipine in synthetic mixture form. Chromatographic separation achieved isocratically on Prontosil C18 column (5µm, 250mm × 4.60mm) and acetonitrile: 20mM Phosphate buffer (pH 3.6) in the ratio of 60:40 (v/v) as the mobile phase, at a flow rate of 1 mL/min. Detection was carried out at 278 nm. The mean retention times for metoprolol succinate and lacidipine was found to be 5.25±0.5 and 6.72±0.5 min, respectively. No interference was found by the excipients in the synthetic mixture. Linearity for metoprolol succinate and lacidipine was in the range of 5-25μg/mL and 4-20μg/mL, respectively. The mean recoveries obtained for metoprolol succinate and lacidipine were 100.03 and 99.67 % respectively and RSD was less than 2. The correlation coefficients for all components are close to 1. The proposed method was validated in terms of linearity, range, accuracy, precision, specificity, robustness and the method is successfully applies to the estimation of metoprolol succinate and lacidipine in bulk and in a synthetic mixture.

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Development of a supercritical fluid chromatography–tandem mass spectrometry method for the determination of lacidipine in beagle dog plasma and its application to a bioavailability study

Geng

Zhao

et al. 2014

Journal of Chromatography B

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Liquid chromatography–tandem mass spectrometric assay for the light sensitive calcium channel antagonist lacidipine in human plasma

Cited by 5 publications

References 16 publications

Bioanalysis of riluzole in human plasma by a sensitive LC-MS/MS method and its application to a pharmacokinetic study in South Indian subjects

Bioanalysis of riluzole in human plasma by a sensitive LC-MS/MS method and its application to a pharmacokinetic study in South Indian subjects

Development of a supercritical fluid chromatography–tandem mass spectrometry method for the determination of lacidipine in beagle dog plasma and its application to a bioavailability study

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