Liquid chromatography–tandem mass spectrometric assay for clobetasol propionate in human serum from patients with atopic dermatitis

Pharmacokinetics study about topical clobetasol on oral mucosa

“…on patients with atopic dermatitis (21), Olsen and Cornell found CP serum absorption of about 6–7 ng/ml through normal skin (19), while Sparidans et al. (22), validating the chemical assay for CP identification in human serum using HPLC–tandem mass spectrometry, measured CP blood concentrations of about 1 ng/ml in two patients with severe atopic dermatitis.…”

“…Finally, Spiridans et al. (22) described a chemical analytical assay to detect serum CP levels in two patients using CP ointment to treat severe atopic dermatitis.…”

“…Recently, Kimball et al (21) reported preliminary pharmacokinetics data of CP lotion or ointment to treat eczematous dermatitis, but poorly reliable results were obtained, with a great variability of values. Finally, Spiridans et al (22) described a chemical analytical assay to detect serum CP levels in two patients using CP ointment to treat severe atopic dermatitis.…”

The potency of clobetasol propionate: Serum levels of clobetasol propionate and adrenal function during therapy with 0.05% clobetasol propionate in patients with severe atopic dermatitis

“…Serum clobetasol propionate levels CLO levels were measured using a validated LC/MS/ MS method in the range 0.05-10 ng/ml (9). In short, 500 ml serum, with clobetasone butyrate added as an internal standard, was extracted using hexanediethyl ether (3/1, v/v).…”

Development and Validation of UV Spectrophotometric Method for Quantitative Estimation of Clobetasol 17-Propionate

“…It was also proven to be the first topical corticosteroid that demonstrated satisfactory results in the treatment for psoriasis 2,4 . Literature search reveals HPLC, RP-HPLC and liquid chromatography methods were reported for determination of various salts of Clobetasol in formulations like ointment, creams and suspensions [6][7][8][9][10][11] . Beside these, some simultaneous analytical estimations of Clobetasol 17-Propionate with other drugs have been reported in literature [12][13][14][15][16] .…”