Liquid Chromatography Tandem Mass Spectrometric Analytical Method for Study of Colchicine in Rats Given Low Doses

Abstract

A selective and sensitive assay was developed for colchicine in rat specimens. Colchicine and its deuterated analog (as internal standard, IS) were extracted from rat specimens (minimal 0.1 mL plasma, whole blood, or urine) using liquid-liquid extraction with n-hexane:dichloromethane:isopropanol. The mobile phase (formic acid: ammonium acetate: methanol) was pumped with uniform flow through an octadecylsilane analytical column. Detection was carried out by electrospray positive ionization in the multiple-reaction monitoring mode. The assay (total run time <3 min) had excellent linearity over a wide (400–800-fold) concentration range. The mean absolute recovery was >96.8%. The intra- and inter-day coefficients of variation were ˂15%, with lower limits of quantitation of 0.5 ng/mL in 0.1 mL of rat plasma. The method also provided the same lower limits of quantitation in urine and whole blood with 0.1 mL volumes, and 0.1 ng/mL using 0.5 mL of rat plasma. The blood-to-plasma ratio was >1. Rats had measurable colchicine blood concentrations for at least 24 h after intravenous doses of 0.1 mg/kg. The method possessed suitable measures of sensitivity and selectivity for detecting colchicine in several specimen types in rats given low doses.

Impact of Polyploidy Induction for Salinity Stress Mitigation in Soybean (Glycine max L. Merrill)

“…Progenies, subsequently leading to the formation of diversified species’ mutant varieties [ 6 ], can stably inherit these newly formed genomic features. In leguminous plants and likewise in non-leguminous species such as rice, wheat, tomato, cutleaf groundcherry etc., polyploidization has been widely tested using chemicals such as colchicine [ 7 ], epoxomicin [ 8 ], sodium azide [ 9 ], oryzalin [ 10 ], nitroxide [ 11 ] and ethyl methanesulfonate [ 12 ], some of which are illustrated below in Figure 1 . These chemical compounds cause numerous genetic mutations that result in significant changes in the plant’s nuclear and proteome systems.…”

Salinity Stress Mitigation by Polyploidy Induction in Soybean (Glycine max L. Merrill)

“…Progenies, subsequently leading to the formation of diversified species' mutant varieties [6], can stably inherit these newly formed genomic features. In leguminous plants, likewise in non-leguminous species like rice, wheat, tomato, cutleaf groundcherry etc., polyploidization has been widely tested using chemicals such as colchicine [7], epoxomicin [8], sodium azide [9], oryzalin [10], nitroxide [11] and ethyl methanesulfonate [12], some of which are illustrated below in Figure 1. These chemical compounds cause numerous genetic mutations that result in significant changes on the plant's nuclear and proteome systems.…”

Determination of Colchicine in Pharmaceutical Formulations, Traditional Extracts, and Ultrasonication-Based Extracts of Colchicum autumnale Pleniflorum (L.) Using Regular and Greener HPTLC Approaches: A Comparative Evaluation of Validation Parameters

“…Colchicine (CLH) is an alkaloidal compound that is commonly obtained from Colchicum autumnale Pleniflorum (L.) (family: Colchicaceae) [1]. In the traditional system of medicine, CLH is used in the treatment of gout [2].…”

“…This compound has been analyzed in various biological fluids, such as blood serum, blood plasma, and urine samples, using an HPLC approach [11,20,21]. For its determination in serum and plasma samples, various liquid chromatography-mass spectrometry (LC-MS) methods have been reported [1,[22][23][24]. For the determination of this compound in various commercial formulations and plant extracts, distinct high-performance thin-layer chromatography (HPTLC) assays have been reported [10,[25][26][27][28][29][30][31][32][33][34][35].…”