Liquid chromatography studies on the pharmacokinetics of phentermine and fenfluramine in brain and blood microdialysates after intraperitoneal administration to rats

Kaddoumi, Amal; Nakashima, Mihoko N.; Maki, Toshihide; Matsumura, Yoshihiro; Nakamura, Junzo; Nakashima, Kenichiro

doi:10.1016/s1570-0232(03)00231-9

Cited by 23 publications

(32 citation statements)

References 42 publications

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“…Norf-HCl and NFen were synthesized from Fen-HCl as previously described [12]. 4-(4,5-Diphenyl-1H-imidazol-2-yl)benzoyl chloride (DIB-Cl) was synthesized in our laboratory [13] and can be obtained from Tokyo Kasei Kogyo (Tokyo, Japan).…”

Section: Chemicalsmentioning

confidence: 99%

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In vivo incorporation of fenfluramine and norfenfluramine into pigmented and nonpigmented hair of rats measured by HPLC-fluorescence detection

2010

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Section: Chemicalsmentioning

confidence: 99%

“…Plasma samples were extracted as described previously [12]. In brief, to 50 l of plasma, 100 l borate buffer (0.1 M, pH 10.6) and ethyl acetate were added.…”

Section: Sample Pretreatmentsmentioning

confidence: 99%

In vivo incorporation of fenfluramine and norfenfluramine into pigmented and nonpigmented hair of rats measured by HPLC-fluorescence detection

2010

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“…In our study, the combination of HPLC separation with fluorescence detection using our original labeling reagent (DIBCl) and microdialysis sampling were used for estimation for drug-drug interactions of abusable drugs. 58,[70][71][72] MDMA tablets often contain other active components having hallucinogenic and/or stimulant effect such as caffeine, ketamine, ephedrine or methamphetamine. After administration of MDMA (5 mg/kg, i.p.)…”

Section: ·2 Evaluation Of Drug-drug Interaction Potential Formentioning

confidence: 99%

“…The consequence of such alteration in the pharmacokinetics of a CNS drug may play a part in the enhancing effect as well as in the neurotoxicity of Phen and Fen. 71 PPA is a synthetic sympathomimetic amine available as a nasal decongestant and is also used as an appetite suppressant in the USA. In Japan, PPA was only available as a decongestant of OTC products by combination with caffeine and/or chlorphenylamine.…”

Section: ·2 Evaluation Of Drug-drug Interaction Potential Formentioning

confidence: 99%

Analytical Studies on the Development of High-Performance Liquid Chromatographic Methods with Fluorescence or Chemiluminescence Detections and Their Practical Applications

2009

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This review describes a comprehensive investigation focusing on syntheses of analytical reagents, followed by their utilization in development of analytical methods, and leading to practical applications for rational use of medicaments centering on abused drugs. Many kinds of analytical reagents for fluorescence and chemiluminescence detections have been synthesized with the intention of improving sensitivity. By properly combining the developed analytical reagents with an HPLC separation technique, one could determine ultra-small amounts of abused drugs such as stimulants, narcotics and obesity drugs in various matrices. Furthermore, chiral analyses of some abused drugs and evaluation of their potential for drug-drug interaction were also performed. The developed methods might be useful for forensic and toxicological studies on drug abuse. Also, the results obtained in our study might contribute to the prediction of and the protection of human health from risks of abused drugs.

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“…DIB-Cl has proven to be a useful derivatization reagent for the HPLC-FL analysis of primary and secondary amines such as amphetamine, methamphetamine, and other sympathomimetic amines as well as bisphenol A, atomoxetine, and various other compounds [17][18][19][20][21][22]. The high fluorescence quantum yield, high selectivity and mild derivatization reaction conditions are considered to be the merits of DIB-Cl derivatization assay.…”

Section: Reaction Mechanismmentioning

confidence: 99%

A novel HPLC fluorescence method for the quantification of methylphenidate in human plasma

Zhu

Wang

Patrick

et al. 2007

Journal of Chromatography B

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A number of analytical methods have been established to quantify methylphenidate (MPH). However, to date no HPLC methods are applicable to human pharmacokinetic studies without the use of mass spectrometry (MS) detection. We developed a sensitive and reliable HPLC-fluorescence method for the determination of MPH in human plasma using 4-(4,5-diphenyl-1H-imidazol-2-yl) benzoyl chloride (DIB-Cl) as the derivatizing agent. An established GC-MS method was adopted in this study as a comparator assay. MPH was derivatized DIB-Cl, and separated isocratically on a C18 column using a HPLC system with fluorescence detection (λex: 330 nm, λem: 460 nm). The lower limit of determination was found to be 1 ng/mL. A linear calibration curve was obtained over the concentrations ranging from 1 to 80 ng/mL (r=0.998). The relative standard deviations of intra-day and inter-day variations were ≤ 9.10 % and ≤ 7.58 %, respectively. The accuracy ranged between 92.59 % and 103.06 %. The method was successfully applied to the pharmacokinetic study of a subject who received a single oral dose (0.3 mg/kg) of immediate-release MPH and yielded consistent results with that of the GC-MS method. This method is the first HPLC assay with non-MS detection providing sufficient reliability and sensitivity for both pre-clinical and clinical studies of MPH.

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Liquid chromatography studies on the pharmacokinetics of phentermine and fenfluramine in brain and blood microdialysates after intraperitoneal administration to rats

Cited by 23 publications

References 42 publications

In vivo incorporation of fenfluramine and norfenfluramine into pigmented and nonpigmented hair of rats measured by HPLC-fluorescence detection

In vivo incorporation of fenfluramine and norfenfluramine into pigmented and nonpigmented hair of rats measured by HPLC-fluorescence detection

Analytical Studies on the Development of High-Performance Liquid Chromatographic Methods with Fluorescence or Chemiluminescence Detections and Their Practical Applications

A novel HPLC fluorescence method for the quantification of methylphenidate in human plasma

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