Liquid chromatography on soft packing material, under axial compression

Danilov, Andrey; Vagenina, I.V.; Mustaeva, Leila G.; Мошников, С. А.; Gorbunova, E. Yu.; Cherskii, V.V.; Baru, M.B.

doi:10.1016/s0021-9673(97)00034-4

Cited by 9 publications

(4 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The empirical approach of this model does neglect two physical phenomena of bed compression: axial compression gradients (6, 12) and particle deformation (13). The model proposed in this study requires the assumption that bed compression is the result of uniform particle reconfiguration only.…”

Section: Resultsmentioning

confidence: 99%

Pressure-Flow Relationships for Packed Beds of Compressible Chromatography Media at Laboratory and Production Scale

2001

View full text Add to dashboard Cite

Pressure drop across chromatography beds employing soft or semirigid media can be a significant problem in the operation of large-scale preparative chromatography columns. The shape or aspect ratio (length/diameter) of a packed bed has a significant effect on column pressure drop due to wall effects, which can result in unexpectedly high pressures in manufacturing. Two types of agarose-based media were packed in chromatography columns at various column aspect ratios, during which pressure drop, bed height, and flow rate were carefully monitored. Compression of the packed beds with increasing flow velocities was observed. An empirical model was developed to correlate pressure drop with the aspect ratio of the packed beds and the superficial velocity. Modeling employed the Blake-Kozeny equation in which empirical relationships were used to predict bed porosity as a function of aspect ratio and flow velocity. Model predictions were in good agreement with observed pressure drops of industrial scale chromatography columns. A protocol was developed to predict compression in industrial chromatography applications by a few laboratory experiments. The protocol is shown to be useful in the development of chromatographic methods and sizing of preparative columns.

show abstract

Section: Resultsmentioning

confidence: 99%

Pressure-Flow Relationships for Packed Beds of Compressible Chromatography Media at Laboratory and Production Scale

2001

View full text Add to dashboard Cite

show abstract

“…The empirical approach taken does neglect two physical phenomena of bed compression: axial compression gradients (5) and particle deformation (16). These effects likely play a more important role in the vicinity of the critical velocity.…”

Section: Resultsmentioning

confidence: 99%

“….91 e 1304u 10% ) (11) (5) and particle deformation (16). These effects likely play a more important role in the vicinity of the critical velocity.…”

Section: Development Of An Empirical Correlation To Account For the Ementioning

confidence: 99%

A Framework for the Prediction of Scale‐Up When Using Compressible Chromatographic Packings

Tran

Joseph

Sinclair³

et al. 2007

Biotechnology Progress

View full text Add to dashboard Cite

Experimental data are given for the solid pressure distributions in chromatography columns of various column aspect ratios packed with four types of agarose-based resin. The loss of column wall support at large scales can result in unexpectedly high pressures caused by the compression of the matrix via drag forces exerted by fluid flow through the bed. The need for an accurate model to predict flow conditions at increasing scale is essential for the scaling-up of chromatographic processes and for avoiding bed compression during operation. Several studies have generated correlations that allow for the prediction of column pressure drops, but they either are mathematically complex, which impairs their practical use, or require a large number of experiments to be performed before they can be used. In this study an empirical correlation was developed based on a previously proposed model, which links the critical velocity of operation of a chromatographic system (microcrit), to the gravity-settled bed height (L0), the column diameter (D), the feed viscosity (micro), and the compressibility of the chromatographic media used (micro 10%). The methodology developed in this study is straightforward to use and significantly reduces the burden of preceding laboratory-scale experimentation. The approach can be used to predict the critical velocity of any chromatographic system and will be useful in the development of chromatographic operations and for column sizing.

show abstract

“…Column pressure may increase from increased flowrate or increased downflow resistance such as blocked frits and protein gelation 25. A progressive collapse results in reduction of the bed void fraction 26. This, in turn, shifts the concentration bands in the columns substantially, resulting in degradation of the system performance.…”

Section: Analysis Methodologymentioning

confidence: 99%

Analysis of zone and pump configurations in simulated moving bed purification of insulin

et al. 2006

View full text Add to dashboard Cite

Liquid chromatography on soft packing material, under axial compression

Cited by 9 publications

References 9 publications

Pressure-Flow Relationships for Packed Beds of Compressible Chromatography Media at Laboratory and Production Scale

Pressure-Flow Relationships for Packed Beds of Compressible Chromatography Media at Laboratory and Production Scale

A Framework for the Prediction of Scale‐Up When Using Compressible Chromatographic Packings

Analysis of zone and pump configurations in simulated moving bed purification of insulin

Contact Info

Product

Resources

About