Liquid chromatography of sugars on silica-based stationary phases

Verhaar, L.A.Th.; Kuster, B.F.M.

doi:10.1016/s0021-9673(00)81928-7

Cited by 95 publications

(11 citation statements)

References 41 publications

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“…Sugars including glucose, fructose and sucrose were determined by high performance liquid chromatography (HPLC) with a $ondapal&arbohy-drate column (30 cm x 3.9 mm i.d.) (Waters Associates) (Verhaar and Kuster, 1981). The mobile phase was CH3CN.H20 = 75:25 and the detector was a differential refractometer (Waters, Model 401).…”

Section: Methodsmentioning

confidence: 99%

Limonin and Naringin Removal from Grapefruit Juice with Naringinase Entrapped in Cellulose Triacetate Fibers

Tsen

1991

Journal of Food Science

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Naringin and limonin are two bitter components of some citrus products such as grapefruit juice. Naringin could be removed by hydrolysis with immobilized naringinase and limonin might be removed by adsorption with cellulose monoacetate gel beads. Cellulose triacetate fibers show a similar limonin adsorption capacity as cellulose monoacetate gel beads. Thus when naringinase from Penicillium sp. was entrapped in such fibers, an enzyme column was made which could remove both bitter components simultaneously. When grapefruit juice was debittered with this enzyme column, sugar components, total organic acids and turbidity were not affected. The enzyme column could be regenerated by washing with warm water. In addition, operational stability of the enzyme column was satisfactory. Such enzyme column could be considered for industrial use.

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Section: Methodsmentioning

confidence: 99%

Limonin and Naringin Removal from Grapefruit Juice with Naringinase Entrapped in Cellulose Triacetate Fibers

Tsen

1991

Journal of Food Science

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“…2). [89,90] However, the detection of LR-CD by RI has the disadvantage of a limited sensitivity and does not allow the gradient elutions necessary for the separation of complex mixtures of LR-CD with a high DP. High performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) has been recently introduced for the analysis of LR-CD.…”

Section: Liquid Chromatographymentioning

confidence: 99%

Enzymatic Synthesis and Analysis of Large-Ring Cyclodextrins

Zheng¹,

Endō²,

Zimmermann³

2002

Aust. J. Chem.

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A Al ll l e en nq qu ui ir ri ie es s a an nd d m ma an nu us sc cr ri ip pt ts s s sh ho ou ul ld d b be e d di ir re ec ct te ed d t to o: : Volume 55, 2002 © CSIRO 2002 publishing research papers from all fields of chemical science, including synthesis, structure, new materials, macromolecules, supramolecular chemistry, biological chemistry, nanotechnology, surface chemistry, and analytical techniques.Large-ring cyclodextrins (LR-CD) are cyclic α-1,4-glucans composed of nine to more than several hundred glucopyranose units. Cyclodextrins are able to form highly specific inclusion complexes with a wide range of guest molecules thereby modifying their physicochemical properties. Only in the past 10 years, LR-CD with a degree of polymerization between 9 and 32 have been isolated and characterized. The structures of these LR-CD show novel features distinct from those of the extensively studied α-, β-, and γ-CD composed of six, seven, and eight glucopyranose units, respectively. Recent work on the enzymatic synthesis of LR-CD has confirmed the ability of different 4-α-glucanotransferases to form large cyclic α-1,4-glucans from amylose and amylopectin, depending on the specificity of the enzyme used and on the substrate and reaction conditions chosen. The potential of LR-CD for specific host-guest interactions and corresponding applications has been demonstrated. . His fields of research include: enzyme technology for the modification and synthesis of carbohydrates from renewable biomass and related industrial applications, molecular recognition, analytical methods for the analysis of carbohydrates, novel enzymes and their application in biotechnology, biotechnology in the paper and textile industry.

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“…The elution sequence using a NH 2 -bonded silica and acetonitrile-water system followed the order of molecular size, and the elution time gave qualitative information about the molecular size of the sugar. 26 It became apparent that the mono-branched bCD 13 was distinguishable from the di-branched bCDs 10-12. In contrast, in C 18 -bonded silica, a reversed phase, the separation mechanism is probably based on hydrophobic chromatography; that is, increased retention with decreasing solubility in water.…”

Section: Introductionmentioning

confidence: 99%