Liquid chromatography of active principles in Sophora flavescens root

Chen, Xi; Yi, Changqing; Yang, Xiaoqing; Wang, Xiaoru

doi:10.1016/j.jchromb.2004.08.032

Cited by 47 publications

(23 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It seems that the alkaloids other than matrine‐type may contribute to the anti‐neoplastic activities of SF alkaloids. Previous reports indicate that both aloperine and cytisine are also the characterized components in SF [9,17,18], but inspection of the literature data reveals that the potential anti‐tumour activities of these alkaloids have not been carefully examined.…”

mentioning

confidence: 99%

In Vitro Anti‐Tumour Activities of Quinolizidine Alkaloids Derived from Sophora Flavescens Ait

Lin

Huang

Liu

et al. 2010

Basic Clin Pharma Tox

View full text Add to dashboard Cite

Abstract:The dry root of Sophora flavescens Ait. (SF) has long been used in a variety of Chinese herbal formulations to treat patients with cancer. Alkaloids are commonly known to present in SF as main active constituents. Here, we report that among the six characterized SF-derived quinolizidine alkaloids including sophoridine, aloperine, sophocarpine, matrine, oxymatrine and cytisine, aloperine exerted the most potent in vitro cytotoxic activity against the human cancer cell lines and oxymatrine exhibited selective anti-cancer activity against hepatocellular carcinoma HepG2 cells. Analysis of DNA fragmentation and PARP cleavage revealed that aloperine treatment for 48 hr induced apoptosis in HL-60 cells. In addition, autophagic formation of acidic vacuole was also observed in HL-60 cells exposed to aloperine. These results suggest that aloperine may be a novel contributor to the anti-cancer properties of SF.The dry root of Sophora flavescens Ait. (SF) (Leguminosae), also referred to as Kushen, has a long history of use in a variety of traditional Chinese medicine herbal formulations to treat a range of diseases, including cancer, viral hepatitis, cardiac arrhythmia and skin diseases [1-4]. As higher percentage of alkaloids is found in SF (3.3%), SF alkaloids have received much attention in the past decades in China as the bioactive substances [5,6]. For example, SF alkaloids are demonstrated to be the potential chemopreventive and chemotherapeutic agents, and they contribute to the therapeutic anti-cancer activity of SF. Alkaloids injection prepared from SF has been approved to treat patients with cancer by the Chinese State Food and Drug Administration (SFDA) in 1992. Clinical studies have demonstrated that the injection is a safe and effective remedy for the treatment of various types of cancer [7,8].Quinolizidine alkaloids have been identified to be the major components of SF alkaloids [9]. Quinolizidine alkaloids represent about 2% of the known alkaloids from plants [10], and most of them were initially noticed as constituents of plants poisonous to humans and livestock. However, accumulated evidence indicates that some of them exhibit potentially useful pharmacological properties like anti-cancer, anti-bacterium, anti-virus, anti-inflammation and pain relief [11]. Quinolizidine alkaloids can be divided into more than six structural groups such as matrine-type, aloperinetype and cytisine-type. Matrine-type alkaloids such as matrine, oxymatrine, sophocarpine and sophoridine are commonly purified from SF [9]. Although previous studies demonstrate that matrine-type alkaloids mediate growth inhibition in different types of cancer cells, the reported IC 50 values are generally not lower than 0.5 mM [12][13][14][15][16]. It seems that the alkaloids other than matrine-type may contribute to the anti-neoplastic activities of SF alkaloids. Previous reports indicate that both aloperine and cytisine are also the characterized components in SF [9,17,18], but inspection of the literature data reveals that the potential ...

show abstract

mentioning

confidence: 99%

In Vitro Anti‐Tumour Activities of Quinolizidine Alkaloids Derived from Sophora Flavescens Ait

Lin

Huang

Liu

et al. 2010

Basic Clin Pharma Tox

View full text Add to dashboard Cite

show abstract

“…The matrine-type alkaloid, sophocarpine, is a biologically active compound derived from the foxtail-like sophora herb and seed (Chen et al, 2004;Wu et al, 2006;Yang et al, 1998). The chemical structure of sophocarpine is shown in Fig.…”

Section: Introductionmentioning

confidence: 99%

Intestinal transport of sophocarpine across the Caco‐2 cell monolayer model and quantification by LC/MS

Sun

Zhang

Sun

et al. 2014

Biomedical Chromatography

View full text Add to dashboard Cite

Sophocarpine is a biologically active component obtained from the foxtail-like sophora herb and seed that is often orally administered for the treatment of cancer and chronic bronchial asthma. The aim of this study was to develop a rapid and specific LC/MS method for the determination of sophocarpine and to explore its transcellular transport mechanism across the Caco-2 (the human colon adenocarcia cell lines) monolayer cell transwell model. Caco-2 cells were seeded on permeable polycarbonate membranes and incubated for 21 days. Before the experiment, the trans-epithelial electric resistance, integrity and alkaline phosphatase activity of the Caco-2 monolayers were verified and used in subsequent experiments. In the Caco-2 model constructed, many influencing factors were investigated, including time, concentration, pH and different protein inhibitors. The results suggested that sophocarpine was transported mainly by passive diffusion. The flux of sophocarpine was time- and concentration-dependent, and the pH also had an effect on its transportation. The PappBA was higher than PappAB , indicating that a polarized transport might exist for sophocarpine. MK-571 and reserpine, inhibitors of the multidrug resistance associated protein 2 and the breast cancer resistance protein, decreased the efflux of sophocarpine, while verapamil had no effect on its transport. These results revealed that sophocarpine is absorbed mainly by passive diffusion, and that a carrier-mediated mechanism is also involved in the transport of sophocarpine.

show abstract

“…The dried roots of S. flavescens, which contain many alkaloids such as matrine and oxymatrine (Liu et al, 2011), are commonly used to treat viral hepatitis, cancer, viral myocarditis, gastrointestinal hemorrhage and skin diseases (Chen et al, 2004; Ling et al, 2007; Cao et al, 2011). They also contain many flavonoids that have antioxidative potential and beneficial effects on human health (Piao et al, 2006; Jeong et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Karyological, morphological and phytochemical characteristics of medicinal plants Sophora flavescens Aiton grown from seeds collected at different localities

2014

View full text Add to dashboard Cite

BackgroundDried roots of Sophora flavescens Aiton contain many phytochemicals that exhibit beneficial effects on human health. This study examined and compared the karyological, morphological and phytochemical characteristics of three S. flavescens populations collected from the Danda, Hualien and Yuli of Taiwan and a population collected from Gansu, China.ResultsKaryotypes of the four populations were similar, with a diploid number of 2n = 18. The Hualien population produced more roots but with less matrine and oxymatrine contents in its root tissue than others. However, only the root of Danda population had a measurable level of naringenin. The dried root of Yuli population had greater ferric reducing antioxidant power and trolox equivalent antioxidant capacity than that of the other populations.ConclusionsThus, the collected S. flavescens populations, particularly the population collected from Danda, have the potential to be used in breeding programs.Electronic supplementary materialThe online version of this article (doi:10.1186/1999-3110-55-5) contains supplementary material, which is available to authorized users.

show abstract

Liquid chromatography of active principles in Sophora flavescens root

Cited by 47 publications

References 24 publications

In Vitro Anti‐Tumour Activities of Quinolizidine Alkaloids Derived from Sophora Flavescens Ait

In Vitro Anti‐Tumour Activities of Quinolizidine Alkaloids Derived from Sophora Flavescens Ait

Intestinal transport of sophocarpine across the Caco‐2 cell monolayer model and quantification by LC/MS

Karyological, morphological and phytochemical characteristics of medicinal plants Sophora flavescens Aiton grown from seeds collected at different localities

Contact Info

Product

Resources

About