Liquid chromatography/mass spectrometry stable isotope analysis of dissolved organic carbon in stream and soil waters

Albéric, Patrick

doi:10.1002/rcm.5212

Cited by 22 publications

(22 citation statements)

References 24 publications

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“…The same degree of correlation was found by Boschker et al ., when he compared the δ 13 C values measured with HPLC/IRMS with values determined with an EA/IRMS system for different carbohydrates . In the investigation by Albéric the maximum difference between the targeted and measured δ 13 C values of the reference standards did not exceed 0.5 ‰ . As expected, when we compared two measurement systems, we observed an offset in the δ 13 C values for the standard substances and the DOM samples between the HPLC/IRMS system and the EA/IRMS system, which needed to be corrected.…”

Section: Discussionmentioning

confidence: 51%

“…Interest in estimating the role of DOM for carbon transport, storage, release and finally for the determination of the net ecosystem balances has increased in recent years . HPLC/IRMS can be used to determine the quantity and isotopic content of bulk DOC in soil water samples . In our investigation, the modified HPLC/IRMS system was characterized by higher background stability and more accurate measurements .…”

Section: Discussionmentioning

confidence: 97%

“…We also evaluated the values of the isotopic signature determined with HPLC/IRMS against an alternative measurement (EA/IRMS) system. Albéric recently introduced the use of HPLC/IRMS to measure quantity and isotopic signature of DOC samples . However, only one natural complex macromolecular standard (a fulvic acid) was used as the δ 13 C working standard for the DOC measurements in that study, which assumed the same (complete) conversion efficiency for all molecules in the DOC samples.…”

Section: Discussionmentioning

confidence: 99%

“…HPLC/IRMS is now an established method for the analysis of specific compounds like carbohydrates, amino acids or mononucleotides, but not for complex solutions containing unknown compounds . Recently, Albéric introduced the first application of HPLC/IRMS for measurements of DOC in stream and soil waters …”

mentioning

confidence: 99%

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Simultaneous determination of the quantity and isotopic signature of dissolved organic matter from soil water using high‐performance liquid chromatography/isotope ratio mass spectrometry

Scheibe

Krantz

Gleixner

2011

Rapid Comm Mass Spectrometry

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We assessed the accuracy and utility of a modified high-performance liquid chromatography/isotope ratio mass spectrometry (HPLC/IRMS) system for measuring the amount and stable carbon isotope signature of dissolved organic matter (DOM) <1 µm. Using a range of standard compounds as well as soil solutions sampled in the field, we compared the results of the HPLC/IRMS analysis with those from other methods for determining carbon and (13)C content. The conversion efficiency of the in-line wet oxidation of the HPLC/IRMS averaged 99.3% for a range of standard compounds. The agreement between HPLC/IRMS and other methods in the amount and isotopic signature of both standard compounds and soil water samples was excellent. For DOM concentrations below 10 mg C L(-1) (250 ng C total) pre-concentration or large volume injections are recommended in order to prevent background interferences. We were able to detect large differences in the (13)C signatures of soil solution DOM sampled in 10 cm depth of plots with either C3 or C4 vegetation and in two different parent materials. These measurements also demonstrated changes in the (13)C signature that demonstrate rapid loss of plant-derived C with depth. Overall the modified HLPC/IRMS system has the advantages of rapid sample preparation, small required sample volume and high sample throughput, while showing comparable performance with other methods for measuring the amount and isotopic signature of DOM.

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Section: Discussionmentioning

confidence: 51%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Simultaneous determination of the quantity and isotopic signature of dissolved organic matter from soil water using high‐performance liquid chromatography/isotope ratio mass spectrometry

Scheibe

Krantz

Gleixner

2011

Rapid Comm Mass Spectrometry

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“…Bulk DOC and δ 13 C (δ 13 C DOC ) were measured with either a customised Thermo HiperTOC coupled to a Delta + XL IRMS (Bouillon and others 2006), or by manual injection in a Thermo IsoLink HPLC-IRMS (similar to the method described in Albéric 2011). …”

Section: Methodsmentioning

confidence: 99%

Disproportionate Contribution of Riparian Inputs to Organic Carbon Pools in Freshwater Systems

et al. 2014

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A lack of appropriate proxies has traditionally hampered our ability to distinguish riverine organic carbon (OC) sources at the landscape scale. However, the dissection of C4 grasslands by C3-enriched riparian vegetation, and the distinct carbon stable isotope signature (δ13C) of these two photosynthetic pathways, provides a unique setting to assess the relative contribution of riparian and more distant sources to riverine C pools. Here, we compared δ13C signatures of bulk sub-basin vegetation (δ13CVEG) with those of riverine OC pools for a wide range of sites within two contrasting river basins in Madagascar. Although C3-derived carbon dominated in the eastern Rianala catchment, consistent with the dominant vegetation, we found that in the C4-dominated Betsiboka basin, riverine OC is disproportionately sourced from the C3-enriched riparian fringe, irrespective of climatic season, even though δ13CVEG estimates suggest as much as 96% of vegetation cover in some Betsiboka sub-basins may be accounted for by C4 biomass. For example, δ13C values for river bed OC were on average 6.9 ± 2.7‰ depleted in 13C compared to paired estimates of δ13CVEG. The disconnection of the wider C4-dominated basin is considered the primary driver of the under-representation of C4-derived C within riverine OC pools in the Betsiboka basin, although combustion of grassland biomass by fire is likely a subsidiary constraint on the quantity of terrestrial organic matter available for export to these streams and rivers. Our findings carry implications for the use of sedimentary δ13C signatures as proxies for past forest-grassland distribution and climate, as the C4 component may be considerably underestimated due to its disconnection from riverine OC pools.Electronic supplementary materialThe online version of this article (doi:10.1007/s10021-014-9772-6) contains supplementary material, which is available to authorized users.

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A novel high‐temperature combustion based system for stable isotope analysis of dissolved organic carbon in aqueous samples. I: development and validation

Federherr

Cerli

Kirkels

et al. 2014

Rapid Comm Mass Spectrometry

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The novel HTC system coupled to an isotope ratio mass spectrometer resulted in significantly improved sensitivity. The system is suitable for salt-containing liquids and compounds that are resistant to oxidation, and it offers a large concentration range. A second paper (which follows this one in this issue) will present a more comprehensive assessment of the analytical performance with a broad set of solutions and real samples. This highly efficient TOC stable isotopic analyzer will probably open up new possibilities in biogeochemical carbon cycle research.

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Liquid chromatography/mass spectrometry stable isotope analysis of dissolved organic carbon in stream and soil waters

Cited by 22 publications

References 24 publications

Simultaneous determination of the quantity and isotopic signature of dissolved organic matter from soil water using high‐performance liquid chromatography/isotope ratio mass spectrometry

Simultaneous determination of the quantity and isotopic signature of dissolved organic matter from soil water using high‐performance liquid chromatography/isotope ratio mass spectrometry

Disproportionate Contribution of Riparian Inputs to Organic Carbon Pools in Freshwater Systems

A novel high‐temperature combustion based system for stable isotope analysis of dissolved organic carbon in aqueous samples. I: development and validation

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