Liquid chromatography–mass spectrometry-based quantification of steroidal glycoalkaloids from Solanum xanthocarpum and effect of different extraction methods on their content

Optimisation and validation of ultra-high performance liquid chromatographic-tandem mass spectrometry method for qualitative and quantitative analysis of potato steroidal alkaloids

“…The compounds were separated on a Waters Acquity BEH C18 column (50 x 2.1 mm, particle size 1.7 µm) using 0.5% formic acid in water (Solvent A) and a mixture of acetonitrile, 2-propanol, and formic acid in the ratio of 94.5: 5: 0.5 (solvent B) as described by Paul et al [20]. The following stepwise gradient program was carried out: 0-1 min 10% B, 2-6 min 20.5% B, 7-9 min 30% B, 9.5 min 90% B and 10-11 min 10% B at a flow rate of 0.5 mL/min.…”

Simple and Reliable Methods for the Determination of Three Steroidal Glycosides in the Eight Species of Solanum by Reversed‐phase HPLC Coupled with Diode Array Detection

“…Although there are several analytical methods for the determination of steroidal glycoalkaloids and their aglycones (Laurila et al, 1999;Eanes et al, 2008;Paul et al, 2008;Skarkova et al, 2008;Shanker et al, 2011), but HPLC-photodiode array (PDA) is the most widely used and most versatile method, because it is rapid, accurate, relatively easy-to-use and reproducible. Furthermore, individual steroidal glycosides and their aglycones can be determined without any derivatisation and HPLC equipment is widely availabile around the world.…”

“…Similarly several indiosides from S. violaceum have shown significant proliferation inhibition against Bel-7402 cells (Ma et al, 2006). Although there are several analytical methods for the determination of steroidal glycoalkaloids and their aglycones, such as GC-MS (Laurila et al, 1999), LC-MS (Eanes et al, 2008), MALDI-TOF/MS (Eanes et al, 2008), HPLC-UV and HPTLC (Eanes et al, 2008;Paul et al, 2008;Skarkova et al, 2008;Shanker et al, 2011), but there is no report on the qualitative and quantitative estimation of the three markers, ISD, SM and SN in S. violaceum or in any other species of the genus Solanum.…”

Preparative separation of glycoalkaloids α-solanine and α-chaconine by centrifugal partition chromatography

“…Moreover, the purity of solanine (85%) is not sufficient for further use. Recently, Paul et al developed an efficient LC-MS protocol for preparative scale isolation and quantification of two steroidal alkaloids solamargine and solasonine from Solanum xanthocarpum [11]. However, in addition to the equipment which is not readily available, this method requires the use of formic acid in the mobile phase.…”