Liquid Chromatography–Mass Spectrometry and Chemometric Analysis of <i>Ricinus communis</i> Extracts for Cultivar Identification

Ovenden, Simon P. B.; Pigott, Eloise J.; Rochfort, Simone; Bourne, David J.

doi:10.1002/pca.2519

Cited by 13 publications

(4 citation statements)

References 18 publications

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“…Our results show that RCB-1 to 3 were compartmentalized differently among the embryo and testa tissues from different countries (Ethiopia, Pakistan, and China). For all castor bean sections from nine geographic origins, we did not find either RCB-4 or RCB-as Ovenden et al reported towards the "impala" cultivar from Tanzania (Ovenden et al, 2014). Due to the tissue heterogeneity, matrix coating heterogeneity, poor analyte extraction, and ionization suppression effects, the quantitative analysis of MALDI-MSI is still challenging.…”

Section: Distribution Of Rcbs In Castor Beanscontrasting

confidence: 58%

“…RCB-1 and RCB-2 were commonly found in varying amounts in six different cultivars, while RCB-3 was found only in the "Carmencita" cultivar. After that, Ovenden et al identified RCB-4 and RCB-5 in the castor beans of the "impala" cultivar (Ovenden et al, 2014). Moreover, Fredriksson et al (Fredriksson et al, 2018) developed a method for chemical analysis of forensic attribution markers related to the purification of ricin based on a complex set of biomarkers including carbohydrates, fatty acids, RCBs, ricin, etc.…”

Section: Introductionmentioning

confidence: 99%

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Key defatting tissue pretreatment protocol for enhanced MALDI MS Imaging of peptide biomarkers visualization in the castor beans and their attribution applications

Qin

Han²,

Wang

et al. 2022

Front. Plant Sci.

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IntroductionCastor bean or ricin-induced intoxication or terror events have threatened public security and social safety. Potential resources or materials include beans, raw extraction products, crude toxins, and purified ricin. The traceability of the origins of castor beans is thus essential for forensic and anti-terror investigations. As a new imaging technique with label-free, rapid, and high throughput features, matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) has been gradually stressed in plant research. However, sample preparation approaches for plant tissues still face severe challenges, especially for some lipid-rich, water-rich, or fragile tissues. Proper tissue washing procedures would be pivotal, but little information is known until now.MethodsFor castor beans containing plenty of lipids that were fragile when handled, we developed a comprehensive tissue pretreatment protocol. Eight washing procedures aimed at removing lipids were discussed in detail. We then constructed a robust MALDI-MSI method to enhance the detection sensitivity of RCBs in castor beans.Results and DiscussionA modified six-step washing procedure was chosen as the most critical parameter regarding the MSI visualization of peptides. The method was further applied to visualize and quantify the defense peptides, Ricinus communis biomarkers (RCBs) in castor bean tissue sections from nine different geographic sources from China, Pakistan, and Ethiopia. Multivariate statistical models, including deep learning network, revealed a valuable classification clue concerning nationality and altitude.

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Section: Distribution Of Rcbs In Castor Beanscontrasting

confidence: 58%

Section: Introductionmentioning

confidence: 99%

Key defatting tissue pretreatment protocol for enhanced MALDI MS Imaging of peptide biomarkers visualization in the castor beans and their attribution applications

Qin

Han²,

Wang

et al. 2022

Front. Plant Sci.

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“…The analysis covered the detailed description of the carbohydrate and primary structure variants, and the results were compared to 10 ricin samples sourced from a total of seven different cultivars. Work conducted by Schieltz et al [ 47 ] elucidated the content of ricin in the beans from 18 different cultivars using isotope dilution mass spectrometry, other groups determined the Ricinus communis source cultivar by a LC-MS method [ 48 ] or based on a complex set of biomarkers using chemometric methods [ 49 ]. Our study encompassed novel strategies discovering the molecular features of ricin purified and analysed by applying a broad method portfolio and implementing combinatorial approaches.…”

Section: Discussionmentioning

confidence: 99%

Glycan Profile and Sequence Variants of Certified Ricin Reference Material and Other Ricin Samples Yield Unique Molecular Signature Features

Josuran,

Wenger,

Müller

et al. 2024

Toxins

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A certified reference material of ricin (CRM-LS-1) was produced by the EuroBioTox consortium to standardise the analysis of this biotoxin. This study established the N-glycan structures and proportions including their loci and occupancy of ricin CRM-LS-1. The glycan profile was compared with ricin from different preparations and other cultivars and isoforms. A total of 15 different oligomannosidic or paucimannosidic structures were identified in CRM-LS-1. Paucimannose was mainly found within the A-chain and oligomannose constituted the major glycan type of the B-chain. Furthermore, the novel primary structure variants E138 and D138 and four different C-termini of the A-chain as well as two B-chain variants V250 and F250 were elucidated. While the glycan proportions and loci were similar among all variants in CRM-LS-1 and ricin isoforms D and E of all cultivars analysed, a different stoichiometry for isoforms D and E and the amino acid variants were found. This detailed physicochemical characterization of ricin regarding the glycan profile and amino acid sequence variations yields unprecedented insight into the molecular features of this protein toxin. The variable attributes discovered within different cultivars present signature motifs and may allow discrimination of the biotoxin’s origin that are important in molecular forensic profiling. In conclusion, our data of in-depth CRM-LS-1 characterization combined with the analysis of other cultivars is representative for known ricin variants.

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“…From the acquired data, which include the exact molecular weight and fragment ions, both known and unknown compounds can be preliminarily identified by this processing method. Principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS‐DA) are powerful tools for multiple factor statistical processing, and have been widely used in the screening of characteristic compounds in herbs and for classification (Ardila, Funari, Andrade, Cavalheiro, & Carneiro, ; Kamal et al, ; Ovenden, Pigott, Rochfort, & Bourne, ; Wang et al, ; Wang et al, ). Data processing allows for polytomized variables to be precisely screened out for dimensionality reduction analysis.…”

Section: Introductionmentioning

confidence: 99%

Qualitative and quantitative determination of Atractylodes rhizome using ultra‐performance liquid chromatography coupled with linear ion trap–Orbitrap mass spectrometry with data‐dependent processing

Zhang

Cui

Fan

et al. 2018

Biomedical Chromatography

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A quick and effective workflow based on ultra-performance liquid chromatography coupled with electron spray ionization and LTQ-Orbitrap mass spectrometry (UPLC-LTQ-Orbitrap MS) was established for compositional analysis and screening of the characteristic compounds of three species of Atractylodes rhizome for quality evaluation. This technique was employed to determine the seven main components in Atractylodes rhizome samples. Ultimately, 78 constituents were identified; of these, seven characteristic compounds were selected for species discrimination, comprising atractylodin (63), atractylenolide I (43), atractylenolide II (49), atractylenolide III (53), atractylon (69), methyl-atractylenolide II (54) and (4E,6E,12E)-tetradecadecatriene-8,10-diyne-1,3-diacetate (59). The seven main compounds, including six characteristic compounds, were simultaneously determined in 29 batches of Atractylodes rhizome samples. Thus, the method validation showed acceptable results. Quantitative analysis showed significantly different contents of the seven main components among the three species of Atractylodes rhizome, which indicates possible distinctions in the pharmacological effects. This established method can simultaneously provide qualitative and quantitative results for compositional characterization of Atractylodes rhizomes and for quality control.

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Liquid Chromatography–Mass Spectrometry and Chemometric Analysis of Ricinus communis Extracts for Cultivar Identification

Cited by 13 publications

References 18 publications

Key defatting tissue pretreatment protocol for enhanced MALDI MS Imaging of peptide biomarkers visualization in the castor beans and their attribution applications

Key defatting tissue pretreatment protocol for enhanced MALDI MS Imaging of peptide biomarkers visualization in the castor beans and their attribution applications

Glycan Profile and Sequence Variants of Certified Ricin Reference Material and Other Ricin Samples Yield Unique Molecular Signature Features

Qualitative and quantitative determination of Atractylodes rhizome using ultra‐performance liquid chromatography coupled with linear ion trap–Orbitrap mass spectrometry with data‐dependent processing

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