Liquid chromatography-hydride generation-atomic absorption spectrometry for the speciation of tin in seafoods

Abstract

Liquid chromatography with hydride generation atomic absorption spectrometry as the detection system was applied to the separation and determination of inorganic tin, tributyltin, dibutyltin, monobutyltin, diphenyltin and monophenyltin. A reversed phase C18 column and a methanol/water/acetic acid (70:27:3, v/v/v) mixture containing 0.05%(v/v) triethylamine and 0.1%(w/v) tropolone as the mobile phase (isocratic elution) were used. Extraction of organotins from the samples was carried out using methanol containing 0.05%(w/v) tropolone, a process that was repeated twice. The supernatants were shaken with water and dichloromethane in a separating funnel and the organic phase was collected and evaporated to dryness. When the method was applied to the speciation of tin in fresh and canned mussels, no organotins above the detection limits were identified in any of the samples, inorganic tin being the only species detected. The reliability of the procedure was checked by analyzing the total tin content of the samples by electrothermal atomic absorption spectrometry and by speciation of tin in a certified reference material, mussel tissue (CRM 477). The method can be used for environmental monitoring of organotins contaminated samples.

Development of a continuous flow hydride generation laser-induced breakdown spectroscopic system: Determination of tin in aqueous environments

“…These elements can also be determined in the form of their volatile hydrides by using a type of sample introduction technique called hydride generation (HG) [1,2]. There is a great interest in the field of analytical chemistry in exploring the analytical capabilities of HG with respect to speciation analysis [3][4][5] and multi-element determinations [6,7] since its first use in 1968 by Holak [8].…”

Atomic spectrometry update. Advances in atomic emission, absorption and fluorescence spectrometry, and related techniques

“…They also discovered that the arsenobetaine concentrations remained unchanged when the baby foods were stored for different times or when the samples were freeze-dried, confirming the stability of arsenobetaine and the non-appearance of other arsenic species by interconversion. They also applied 379 the same technique to the speciation of tin in sea-foods. However, they found no organo-Sn compounds in their samples of fresh and canned mussels.…”

Determination of total tin and organotin compounds in shellfish by ICP-MS

“…However, the derivation step required for GC analysis often results in yield irreproducibility because of matrix interference. High-performance liquid chromatography (HPLC) (Vinas, Lopez-Garcia, Merino-Merono, Campillo, & Hernandez-Cordoba, 2004) does not involve a derivation step, which can eliminate a potential source of uncertainty in the final result. The determination of OTs is usually performed by means of HPLC coupled with various detectors, such as AAS (Minganti, Capelli, & Pellegrini, 1995), AES (Munoz et al, 2005a(Munoz et al, , 2005b and MS (Chiron, Roy, Cottier, & Jeannot, 2000).…”