Liquid chromatography/electrospray mass spectrometry of bioactive terpenoids inGinkgo biloba L.

Mauri, Pierluigi; Migliazza, Barbara; Pietta, Piergiorgio

doi:10.1002/(sici)1096-9888(199912)34:12<1361::aid-jms895>3.0.co;2-6

Cited by 43 publications

(13 citation statements)

References 18 publications

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“…The commercial standardized extracts of G. biloba leaves contains not less then 6% of ginkgolides (a group of unique diterpenes) and bilobalide (a sesquiterpene) and 24% of flavonoids (primarily composed of quercetin, kaempferol, and isorhamnetin glycosides) [7]. Extracts from Gingko biloba leaves are reported to have antioxidant properties attributed primarily to the presence of flavonoid-glycosides in the extract [8].…”

Section: Introductionmentioning

confidence: 99%

“…Extracts from Gingko biloba leaves are reported to have antioxidant properties attributed primarily to the presence of flavonoid-glycosides in the extract [8]. Clinical efficacy of Ginkgo is mainly due to terpene lactones, which have different pharmacological activities, such as peripheral vasoregulation [9], platelet-activating factor (PAF) receptor antagonism [10], prevention of membrane damage caused by free radicals [11] and neuroprotective properties [7]. Oral administration of Ginkgo biloba's extract can prevent the decline in muscarinic receptor density in the hippocampus of rats [12], and might have ability to inhibit the degradation of acetylcholine by acetylcholinesterase [13].…”

Section: Introductionmentioning

confidence: 99%

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LC–MS characterization of terpene lactones in plasma of experimental animals treated with Ginkgo biloba extracts

Mauri

Palma

Pozzi

et al. 2006

Journal of Pharmaceutical and Biomedical Analysis

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

LC–MS characterization of terpene lactones in plasma of experimental animals treated with Ginkgo biloba extracts

Mauri

Palma

Pozzi

et al. 2006

Journal of Pharmaceutical and Biomedical Analysis

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“…According™ to™ several™ other™ quantitative™ studies™ [39,40],™ the™ unavailability™ of™ an™ internal™ standard™ for™ the analysis can be overcome by the use of a calibration curve. To prepare stock standard solutions, 0.0006 gr.…”

Section: Electrospray Ionization Mass Spectrometry (Esi-ms) Experimentsmentioning

confidence: 99%

A new methodology for monitoring the activity of cdMMP-12 anchored and freeze-dried on Au (111)

Grasso

Fragai

Rizzarelli

et al. 2007

J. Am. Soc. Mass Spectrom.

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Matrix metalloproteinases (MMPs) are cell-secreted soluble and membrane-tethered enzymes that are capable of degrading extracellular matrix proteins, but also can process a number of bioactive molecules. They are involved in the cleavage of cell surface receptors, but are also thought to play a major role on cell behaviors as well as in diverse physiological and pathological processes, including embryonic development, wound repair, inflammatory diseases, and cancer. For these reasons, it is obvious that a control over MMPs activity is highly desirable. Consequently, the frantic search for new inhibitors has been coupled to the development of high-throughput methods able to rapidly screen the effect of possible MMP inhibitors on the activity of these enzymes. In this scenario, solid-state-based methods play a major role because of their compatibility with array formats that are able to extract more information from smaller sample volumes and offer some important advantages that are not available in the standard solution assays. In this work, the catalytic domain of MMP-12 was immobilized on a gold substrate and the surface coverage was measured by FT-SPR experiments. A new experimental procedure was developed to freeze-dry the anchored molecules and their activity was measured by ESI-MS. The kinetics parameters obtained for the immobilized enzyme are in good accordance with those reported for similar systems in solution. Inhibition of the immobilized molecules was also carried out, demonstrating the applicability of the method for rapid screening of MMP inhibitors. (J Am Soc Mass Spectrom 2007, 18, 961-969)

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“…Electrospray ionization mass spectrometry (ESI-MS), as a powerful tool for the analysis, has been widely applied to the analysis of glycoproteins [1], oligonucleotides [2,3], oligosaccharides [4,5], drugs and drug metabolites [6], environmental contaminants [7,8], and numerous other types of compounds [9]. In atmospheric pressure ion sources, e.g.…”

Section: Introductionmentioning

confidence: 99%

Induced in-source fragmentation pattern of certain novel (1Z,2E)-N-(aryl)propanehydrazonoyl chlorides by electrospray mass spectrometry (ESI-MS/MS)

Attwa

Abdel‐Aziz

Kadi

2013

Chemistry Central Journal

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BackgroundCollision induced dissociation (CID) in the triple quadrupole mass spectrometer system (QQQ) typically yields more abundant fragment ions than those produced with resonance excitation in the presence of helium gas in the ion trap mass spectrometer system (IT). Detailed product ion spectra can be obtained from one stage MS2 scan using the QQQ. In contrast, generating the same number of fragment ions in the ion trap requires multiple stages of fragmentation (MSn) using CID via in-trap resonance excitation with the associated time penalties and drop in sensitivity.ResultsThe use of in-source fragmentation with electrospray ionization (ESI) followed by product ion scan (MS2) in a triple quadrupole mass spectrometer system, was demonstrated. This process enhances the qualitative power of tandem mass spectrometry to simulate the MS3 of ion trap for a comprehensive study of fragmentation mechanisms. A five pharmacologically significant (1Z, 2E)-N-arylpropanehydrazonoyl chlorides (3a-e) were chosen as model compounds for this study. In this work, detailed fragmentation pathways were elucidated by further dissociation of each fragment ion in the ion spectrum, essentially, by incorporating fragmentor voltage induced dissociation (in-source fragmentation) and isolation of fragments in a quadrupole cell Q1. Subsequently, CID occurs in cell, Q2, and fragment ions are analyzed in Q3 operated in product ion mode this process can be referred to as pseudo-MS3 scan mode.ConclusionsThis approach allowed unambiguous assignment of all fragment ions using tandem mass spectrometer and provided adequate sensitivity and selectivity. It is beneficial for structure determination of unknown trace components. The data presented in this paper provide useful information on the effect of different substituents on the ionization/fragmentation processes and can be used in the characterization of this important class of compounds.

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Liquid chromatography/electrospray mass spectrometry of bioactive terpenoids inGinkgo biloba L.

Cited by 43 publications

References 18 publications

LC–MS characterization of terpene lactones in plasma of experimental animals treated with Ginkgo biloba extracts

LC–MS characterization of terpene lactones in plasma of experimental animals treated with Ginkgo biloba extracts

A new methodology for monitoring the activity of cdMMP-12 anchored and freeze-dried on Au (111)

Induced in-source fragmentation pattern of certain novel (1Z,2E)-N-(aryl)propanehydrazonoyl chlorides by electrospray mass spectrometry (ESI-MS/MS)

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