Liquid chromatography–electrospray mass spectrometry (LC–MS) method for determination of esmolol concentration in human plasma

Zuppa, Athena F.; Shi, Heng; Adamson, Peter C.

doi:10.1016/j.jchromb.2003.08.014

Cited by 10 publications

(7 citation statements)

References 10 publications

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“…Nevertheless, also more recently published articles demonstrate the feasibility of screening and confirmatory analysis for β-blockers utilizing GC-MS [152] and GC-MS/MS [153] with average detection limits of 20 ng/mL. Liquid chromatography has proven to be the more adequate solution for the separation of β-receptor blocking agents, and with the availability of efficient atmospheric pressure ionization techniques such as ESI and APCI numerous screening and specified detection procedures have been established for clinical, forensic and doping control purposes [154][155][156][157][158][159][160]. Here, detection limits ranged from 0.02 to 100 ng/mL.…”

Section: β-Receptor Blocking Agentsmentioning

confidence: 99%

Mass Spectrometry in Doping Control Analysis

Thevis¹,

Schänzer

2005

COC

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Mass spectrometry has become the most frequently employed technique in doping control analysis to identify prohibited compounds ever since their use has been banned by international federations. In combination with gas or liquid chromatography and various ionization methods such as electron or chemical ionization, electrospray, atmospheric pressure chemical ionization, atmospheric pressure photo ionization or matrix-assisted laser desorption ionization, numerous applications have been established enabling the sensitive and selective detection of prohibited drugs in matrices such as urine, blood, and hair. The classes of investigated compounds include low molecular weight drugs such as anabolic steroids, diuretics, β 2 -agonists and β-receptor blocking agents, and others as well as high molecular weight therapeutics, for instance plasma volume expanders based on polysaccharide structures (e.g. hydroxyethyl starch and dextran), hemoglobin-based oxygen therapeutics (e.g. Hemopure), or synthetic insulins. Assays for their identification are commonly based on extractive purification, chemical or enzymatic treatment (i.e. derivatization or degradation) followed by chromatographic and mass spectrometric analysis employing various modern analyzers such as quadrupole, ion trap, triple-quadrupole and magnetic-sector mass spectrometers allowing the sensitive and unambiguous qualitative as well as quantitative determination of xenobiotics in elite athletes' doping control samples. Moreover, the administration of drugs naturally occurring in human beings, for instance testosterone, is uncovered by carbon isotope ratio mass spectrometry that enables the differentiation between endogenous and exogenous sources. The comparison of δ-values obtained from endogenously produced steroids independent from testosterone and its metabolism with urinary testosterone and its metabolic products allow the determination of surreptitious applications.

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Section: β-Receptor Blocking Agentsmentioning

confidence: 99%

Mass Spectrometry in Doping Control Analysis

Thevis¹,

Schänzer

2005

COC

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“…Review of literature has revealed that few methods have been reported for the estimation of esmolol hydrochloride. Most HPLC methods reported are useful in estimating esmolol hydrochloride in human plasma [1][2][3][4][5] and biological fluids 6 . One method of estimation of esmolol hydrochloride by capillary electrophoresis has also been reported 7 .…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of a Rapid RP‐HPLC Method for the Estimation of Esmolol Hydrochloride in Bulk and Pharmaceutical Dosage Forms

Somasekhar

Sankar

2010

Journal of Chemistry

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A reverse phase HPLC method is described for the determination of esmolol hydrochloride in bulk and injections. Chromatography was carried on a C18column using a mixture of acetonitrile, 0.05 M sodium acetate buffer and glacial acetic acid (35:65:3 v/v/v) as the mobile phase at a flow rate of 1 mL/min with detection at 275 nm. The retention time of the drug was 4.76 min. The detector response was linear in the concentration of 1-50 μg/mL. The limit of detection and limit of quantification was 0.614 and 1.86 μg/mL respectively. The method was validated by determining its sensitivity, linearity, accuracy and precision. The proposed method is simple, economical, fast, accurate and precise and hence can be applied for routine quality control of esmolol hydrochloride in bulk and injections.

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“…Review of literature has revealed that few methods have been reported for the estimation of Esmolol hydrochloride. Most HPLC methods reported are useful in estimating Esmolol hydrochloride in human plasma [9][10][11][12][13] and biological fluids [14]. HPLC [15,16] methods have been reported for determination of ESM in pharmaceutical injections.…”

Section: Esmolol Hydrochloridementioning

confidence: 99%

Spectrophotometric Determination of Drugs and Pharmaceuticals by Cerium (IV) Amaranth Dye Couple

2013

IOSR-JAC

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Simple, sensitive, accurate, and precise spectrophotometric methods for quantitative determination of drugs, viz., Darifenacin (DAR), Esmolol Hydrochloride (ESM), Montelukast Sodium (MON), Sildenafil citrate (SIL),Terbinafine (TER) and Tramadol Hydrochloride (TRA) were developed. The method of each drug depends upon oxidation of drugs by Ce (IV) (Excess) and estimating the amount of unreacted Ce (IV) by amaranth dye at 523nm. The calibration curves obeyed Beer's law over the concentration range of 1.4-7.0 µg ml-1 (DAR), 2-14 µg ml-1 (ESM), 2-10 µg ml-1 (MON), 20-70 µg ml-1 (SIL), 3-21 µg ml-1 (TER) & 2-14 µg ml-1 (TRA). The methods have been validated in terms of guidelines of ICH and applied to analysis of pharmaceuticals.

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Liquid chromatography–electrospray mass spectrometry (LC–MS) method for determination of esmolol concentration in human plasma

Cited by 10 publications

References 10 publications

Mass Spectrometry in Doping Control Analysis

Mass Spectrometry in Doping Control Analysis

Development and Validation of a Rapid RP‐HPLC Method for the Estimation of Esmolol Hydrochloride in Bulk and Pharmaceutical Dosage Forms

Spectrophotometric Determination of Drugs and Pharmaceuticals by Cerium (IV) Amaranth Dye Couple

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