Liquid chromatography–electrospray mass spectrometric identification of ginsenosides in Panax ginseng roots

Fuzzati, Nicola; Gabetta, Bruno; Jayakar, Kaishik; Pace, Roberto; Peterlongo, F.

doi:10.1016/s0021-9673(99)00463-x

Cited by 165 publications

(143 citation statements)

References 11 publications

Supporting

Mentioning

134

Contrasting

Order By: Relevance

“…ESI MS is an advanced soft ionization technology, which has been widely adopted for analyzing natural compounds [14,15] for rapid qualitation and quantitation [16,17]. Ultraperformance LC (UPLC TM ) is similar to conventional HPLC, but can be distinguished by its ability to provide higher peak capacity, greater resolution, increased sensitivity, and a higher speed of analysis [18,19].…”

Section: Introductionmentioning

confidence: 99%

A rapid and sensitive UPLC‐ESI MS method for analysis of isofraxidin, a natural antistress compound, and its metabolites in rat plasma

Sun

Zhang

et al. 2007

J of Separation Science

View full text Add to dashboard Cite

Isofraxidin is one of the main bioactive constituents in the root of Acanthopanax senticosus, which has antifatigue, antistress, and immuno-accommondating effects. In this study, an ultraperformance LC (UPLC)-ESI MS method was developed for analyzing isofraxidin and its metabolites in rat plasma. The analysis was performed on a UPLC coupled with ESI MS (quadropole MS tandem TOF MS). The lower LOD (LLOD) for isofraxidin was 0.25 ng/mL, the intraday precision was less than 10%, the interday precision was less than 10%, and the extraction recovery was more than 80%. Isofraxidin and two metabolites (M1 and M2) were detected in rat plasma after oral administration of isofraxidin, and the molecular polarities of M1 and M2 were both increased compared to isofraxidin. The metabolites were identified as 5,6-dihydroxyl-7-methoxycoumarin and 5-hydroxyl-6,7-dimethoxycoumarin when subjected to parent ion spectra, product ion spectra, and extract mass and element composition analyses.

show abstract

Section: Introductionmentioning

confidence: 99%

A rapid and sensitive UPLC‐ESI MS method for analysis of isofraxidin, a natural antistress compound, and its metabolites in rat plasma

Sun

Zhang

et al. 2007

J of Separation Science

View full text Add to dashboard Cite

show abstract

“…Recently, HPLC/MS has been applied in species validation of many one-herb medicines, and compound identification. For example: The alkaloids in Coptidis rhizome [6], the flavonoids in Astragali radix [7], the saikosaponins in Bupleuri radix [8], the ginsenosides in Ginseng radix, and American ginseng [9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Application of LC/MS and ICP/MS for establishing the fingerprint spectrum of the traditional Chinese medicinal preparation Gan‐Lu‐Yin

Lin

Lee²,

Chuang³

2006

J of Separation Science

View full text Add to dashboard Cite

We developed a method to analyze the fingerprint spectrum qualitatively and quantitatively for the traditional Chinese herbal medicinal preparation Gan-Lu-Yin with HPLC combined with photodiode array detection, and MS, and to identify the preparation's 14 main components including baicalin, baicalein, oroxylin A-7-O-glucuronide, wogonin-7-O-glucuronide, wogonin, and oroxylin A in Radix Scutellariae; naringin and neohesperidin in Aurantii fructus; liquiritigenin, liquiritin, and glycyrrhizic acid in Radix Glycyrrhizae. In LC/UV assay, a Cosmosil 5C18-MS-II column was used as the stationary phase, and a gradient of potassium dihydrogen phosphate, ACN, and water as the elute solution. The UV detection wavelengths were 250 and 280 nm. In LC/MS assay, a gradient of phosphoric acid, ACN, and water was used as the elute solution, and electrospray positive ion mode ((+)-ESI) as the analytic mode. In order to explore the distribution of trace metal elements effectively in Gan-Lu-Yin, a microwave digestion method was used for sample treatment, and an inductively coupled plasma MS assay was used to analyze fingerprint spectra of the inorganic metals in Gan-Lu-Yin. Combined with fingerprint spectra of organic compounds by LC/UV and LC/MS, it was expected to provide effective quality control in the production of Gan-Lu-Yin.

show abstract

“…Many studies showed that ginsenosides Rg 1 and Re, and ginsenosides Rb 2 and Rb 3 could not be well separated (Fuzzati et al, 1999;Kwon et al, 2001;Li et al, 2005;Wan et al, 2006a). We utilized a different gradient eluent ratio to separate saponins with similar characteristics and retention time, i.e.…”

Section: Methods Validationmentioning

confidence: 99%

Simultaneous determination of saponins in flower buds of Panax notoginseng using high performance liquid chromatography

Gao

Dan

Zhao

et al. 2007

Biomedical Chromatography

View full text Add to dashboard Cite

The flower buds of Panax notoginseng have been commonly used for the treatment of hypertension, vertigo, tinnitus and acute faucitis in China. The amount of total saponins in the flower buds is higher than in any other parts of P. notoginseng. However, the compositions of flower buds have not been quantified clearly until now. A sensitive and efficient high-performance liquid chromatography-ultraviolet (HPLC-UV) method was developed for the first time to simultaneously quantify eight active saponins in the flower buds of P. notoginseng, including notoginsenoside R(1) and ginsenosides Rg(1), Re, Rb(1), Rb(2), Rb(3), Rd and F(2). The analysis was performed on a reversed-phase C(18) column with gradient elution of acetonitrile and 0.01% aqueous formic acid. The proposed method provided good linearity, reproducibility and sensitivity for the simultaneous quantification of the investigated saponins with overall intra- and inter-day precision and accuracy of better than 4.1% (RSD) and higher than 95% (accuracy), respectively. The recoveries for all the saponins determined were in the range 94.7-104.8% with RSD better than 3.1%. Using the optimized method, we were able to analyze samples from different villages of Wenshan Prefecture, China, which is helpful for quality control of flower buds of P. notoginseng.

show abstract

Liquid chromatography–electrospray mass spectrometric identification of ginsenosides in Panax ginseng roots

Cited by 165 publications

References 11 publications

A rapid and sensitive UPLC‐ESI MS method for analysis of isofraxidin, a natural antistress compound, and its metabolites in rat plasma

A rapid and sensitive UPLC‐ESI MS method for analysis of isofraxidin, a natural antistress compound, and its metabolites in rat plasma

Application of LC/MS and ICP/MS for establishing the fingerprint spectrum of the traditional Chinese medicinal preparation Gan‐Lu‐Yin

Simultaneous determination of saponins in flower buds of Panax notoginseng using high performance liquid chromatography

Contact Info

Product

Resources

About