Liquid chromatography combined with mass spectrometry for the investigation of endoglucanase selectivity on carboxymethyl cellulose

Enebro, Jonas; Momcilovic, Dane; Siika‐aho, Matti; Karlsson, Sigbritt

doi:10.1016/j.carres.2009.08.009

Cited by 19 publications

(2 citation statements)

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“…The solubilized sugars produced by the cellulases were mainly recovered as glucose because of the presence of b-glucosidase. Thus, the previously observed selectivity in the product profiles of the different T. reesei endoglucanases (Enebro et al, 2009) could not be observed. Xylose and other sugars were detected at a level lower of at least an order of magnitude.…”

Section: Enzymescontrasting

confidence: 54%

Liquefaction of hydrothermally pretreated wheat straw at high-solids content by purified Trichoderma enzymes

Szijártó

Siika‐aho

Sontag‐Strohm

et al. 2011

Bioresource Technology

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Enzymatic liquefaction was studied by measuring continuously the flowability change of high-solids lignocellulose substrates using a real time viscometric method. Hydrolysis experiments of hydrothermally pretreated wheat straw were carried out with purified enzymes from Trichoderma reesei; Cel7A, Cel6A, Cel7B, Cel5A, Cel12A and Xyn11A. Results obtained at 15% (w/w) solids revealed that endoglucanases, in particular Cel5A, are the key enzymes to rapidly reduce the viscosity of lignocellulose substrate. Cellobiohydrolases had only minor and the xylanase practically no effect on the viscosity. Efficient, fast liquefaction was obtained already at a dosage of 1.5 mg of Cel5A/gdrysolids. Partial replacement or supplementation of Cel5A by the other major hydrolytic enzymes did not improve the liquefaction. The reduction of viscosity did not correlate with the saccharification obtained in the same reaction, suggesting that efficient liquefaction is rather dependent on the site than the frequency of enzymatic cleavages.

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Section: Enzymescontrasting

confidence: 54%

Liquefaction of hydrothermally pretreated wheat straw at high-solids content by purified Trichoderma enzymes

Szijártó

Siika‐aho

Sontag‐Strohm

et al. 2011

Bioresource Technology

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“…After partial hydrolysis, the chemical structures of the resulting oligomers are often analyzed using methods such as mass spectrometry (MS) or nuclear magnetic resonance NMR [ 23 ]. MS analyses have been performed both with matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry [ 13 ] as well as with electrospray ionization (ESI), with or without separation with liquid chromatography [ 12 , 24 , 25 ]. Such analyses have in turn been performed either with or without prior isotopic labeling [ 26 , 27 ].…”

Section: Introductionmentioning

confidence: 99%

Characterization of the substitution pattern of cellulose derivatives using carbohydrate-binding modules

et al. 2014

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BackgroundDerivatized celluloses, such as methylcellulose (MC) and hydroxypropyl methylcellulose (HPMC), are of pharmaceutical importance and extensively employed in tablet matrices. Each batch of derivatized cellulose is thoroughly characterized before utilized in tablet formulations as batch-to-batch differences can affect drug release. The substitution pattern of the derivatized cellulose polymers, i.e. the mode on which the substituent groups are dispersed along the cellulose backbone, can vary from batch-to-batch and is a factor that can influence drug release.ResultsIn the present study an analytical approach for the characterization of the substitution pattern of derivatized celluloses is presented, which is based on the use of carbohydrate-binding modules (CBMs) and affinity electrophoresis. CBM4-2 from Rhodothermus marinus xylanase 10A is capable of distinguishing between batches of derivatized cellulose with different substitution patterns. This is demonstrated by a higher migration retardation of the CBM in acrylamide gels containing batches of MC and HPMC with a more heterogeneous distribution pattern.ConclusionsWe conclude that CBMs have the potential to characterize the substitution pattern of cellulose derivatives and anticipate that with use of CBMs with a very selective recognition capacity it will be possible to more extensively characterize and standardize important carbohydrates used for instance in tablet formulation.

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Current literature in mass spectrometry

2010

J. Mass Spectrom.

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In order to keep subscribers up‐to‐date with the latest developments in their field, John Wiley & Sons are providing a current awareness service in each issue of the journal. The bibliography contains newly published material in the field of mass spectrometry. Each bibliography is divided into 11 sections: 1 Reviews; 2 Instrumental Techniques & Methods; 3 Gas Phase Ion Chemistry; 4 Biology/Biochemistry: Amino Acids, Peptides & Proteins; Carbohydrates; Lipids; Nucleic Acids; 5 Pharmacology/Toxicology; 6 Natural Products; 7 Analysis of Organic Compounds; 8 Analysis of Inorganics/Organometallics; 9 Surface Analysis; 10 Environmental Analysis; 11 Elemental Analysis. Within each section, articles are listed in alphabetical order with respect to author

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Liquid chromatography combined with mass spectrometry for the investigation of endoglucanase selectivity on carboxymethyl cellulose

Cited by 19 publications

References 31 publications

Liquefaction of hydrothermally pretreated wheat straw at high-solids content by purified Trichoderma enzymes

Liquefaction of hydrothermally pretreated wheat straw at high-solids content by purified Trichoderma enzymes

Characterization of the substitution pattern of cellulose derivatives using carbohydrate-binding modules

Current literature in mass spectrometry

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