Liquid chromatography analysis of monosubstituted sulfobutyl ether‐β‐cyclodextrin isomers on porous graphitic carbon

Abstract

The retention behaviour of the three positional isomers of monosubstituted sulfobutyl ether-beta-cyclodextrin was investigated on a porous graphitic carbon (PGC) column. The influence of the mobile phase composition (nature and concentration of organic and electronic modifiers) was studied as well as the effect of column temperature. These hydrophilic and anionic analytes were highly retained on the PGC stationary phase compared to octadecyl bonded phases. The retention is mainly governed by a reversed-phase mechanism with electronic interaction playing a secondary role. An increase in solute retention and efficiency with temperature was observed. Successful isocratic separation with satisfactory baseline resolution of the three isomers of monosubstituted sulfobutyl ether-beta-cyclodextrin was achieved at 75 degrees C on a Hypercarb column by using ammonium acetate as electronic modifier in water-acetonitrile (83:17). The chromatographic methodology developed can be easily used for relative quantification of each isomer within a mixture and can be applied for semi-preparative purification of each one. The evaporative light scattering detector allows the detection of these non UV-visible absorbing molecules.

Mass Spectrometric Quantification of Amphipathic, Polyphenolic Antioxidant of the Pacific Oyster (Crassostrea Gigas)

“…In this case, a charge-induced interaction between graphite carbon and DHMBA might explain the retention. [11][12][13] The theoretical plate of the peak for the Hypercarb column was 13000. We selected the Hypersil GOLD aQ column for quantitative analysis because of its better theoretical plate than that for the Hypercarb column.…”

Development of an HPLC Method for Determination of Related Impurities in Prilocaine Substance

“…The Hypercarb column permits use in the pH range 1-14 and can give a different selectivity as compared to the C 18 columns. Further, the Hypercarb column has the ability to separate positional isomeric compounds [18,19]. Planar molecules give high retention on this column [20].…”