Liquid chromatographic separation of doxycycline and 4-epidoxycycline in a tissue depletion study of doxycycline in turkeys

Croubels, Siska; Vermeersch, Hans; Backer, Patrick De; Santos, María Dolores; Remon, J.P.; Peteghem, Carlos Van

doi:10.1016/s0378-4347(97)00644-0

Cited by 46 publications

(30 citation statements)

References 11 publications

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“…However, the physicochemical properties of enrofloxacin and doxycycline allow them to remain in dispensers. Missed intervals in sanitising the water lines with special cleaners, particularly after finishing a course of antibiotic administration, can lead to traces of antibiotics being constantly administered to chickens which do not require treatment (8,14,15). Chronic exposure to traces of chemotherapeutics and antibiotics may interfere with the actions of other drugs that are intentionally administered for the treatment of animal infections.…”

Section: Introductionmentioning

confidence: 99%

“…Chronic exposure to traces of chemotherapeutics and antibiotics may interfere with the actions of other drugs that are intentionally administered for the treatment of animal infections. Although there have been studies of antibacterial compounds including doxycycline residue concentrations in different animal tissues (8,11,14,15,20,26,31,32,34), there is no information about the possible effects of constant exposure to trace amounts of previously used antibiotics on target antibiotics in chicken tissue. Therefore, this study elucidates the effects of constant trace amounts exposure on the residues of intentionally applied doxycycline in chicken breast, thigh, and liver.…”

Section: Introductionmentioning

confidence: 99%

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Effect of doxycycline concentrations in chicken tissues as a consequence of permanent exposure to enrofloxacin traces in drinking water

Gbylik-Sikorska

Posyniak

Śniegocki

et al. 2016

Journal of Veterinary Research

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Introduction: The main problem in poultry farming is the difficulty in producing food of animal origin without using antibacterial agents. Because most antibacterial compounds are dispensed in water, some water supply systems can be contaminated by antibiotics which are then administered to the animals unintentionally. This can lead to unexpected increases in antibiotic residues in food of animal origin. The aim of the present study was to determine whether the constant exposure of chicken broilers to enrofloxacin affects the withdrawal time of a therapeutic doxycycline that is intentionally administered to the chickens. Material and Methods: The concentrations of doxycycline, enrofloxacin, and ciprofloxacin were determined by LC-MS/MS in muscles and liver of the chickens. Results: Doxycycline residue concentrations in the chicken tissues from the group that received trace amounts of enrofloxacin were nearly 50% greater than those of the group that received only doxycycline. Conclusion: These results indicated that constant exposure to enrofloxacin in trace amounts significantly influences the residual doxycycline concentration in chicken tissues.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of doxycycline concentrations in chicken tissues as a consequence of permanent exposure to enrofloxacin traces in drinking water

Gbylik-Sikorska

Posyniak

Śniegocki

et al. 2016

Journal of Veterinary Research

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“…According to Commission Regulation (EU 37/2010) and EMA Summary Report Doxycycline 2, quantification of DC in animal tissues requires a determination of only parent compound as the residue marker, without its 4-epimer, contrary to other tetracyclines in which case the MRLs are defined as a sum of parent drugs and their 4-epimers (3,11). DC residue studies have been performed in chickens (1), turkeys (8,20), pigeons (9), and pigs (6, 7) with different doses and route of the drug administration. The antibiotics' residue levels reached in organs and the rate of their depletion from tissues depend on the method of administration, animal species, as well as dose and specific drug given (15).…”

Section: Introductionmentioning

confidence: 99%

“…Because DC is one of the most frequently used antibiotic in chickens, it was necessary to develop a suitable method for determination of this compound in chicken tissues. Many studies using analytical methods for determination of DC in animal tissues have been presented (4,5,8,12,17,18,21,22). Most of them did not describe the application of methods in the analysis of DC in samples collected from treated animals.…”

Section: Introductionmentioning

confidence: 99%

LC-MS/MS analysis of doxycycline residues in chicken tissues after oral administration

Gajda¹,

Posyniak²,

Tomczyk

2014

Bulletin of the Veterinary Institute in Pulawy

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For the purpose of quantitative determination of doxycycline (DC) residues in tissues, a sensitive liquid chromatographytandem mass spectrometry (LC-MS/MS) method was developed. The method was used to determine DC residues in chicken tissues (breast and thigh muscle, liver and kidney) after oral administration with drinking water to five-weak-old broiler chickens. The DC was administered for five consecutive days at a therapeutic dose of 10 mg/kg b.w. once a day. The tissues were collected after 6 h, 24 h, 7 d, and 8 d. The method was validated and the decision limit was established for muscle -109.2 µg/kg, for liver -326.1 µg/kg, and for kidney -634.0 µg/kg. The detection limit was 2 µg/kg and the limit of quantification was 5 µg/kg. In a short period after ceasing the treatment, the detected concentrations of DC were much higher than the established maximum residue limit values. The highest residue concentrations of DC were observed in the kidney, followed by the liver and muscle. The lowest concentration of DC was determined in tight muscle.

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“…Moreover, it is found that tetracyclines could be applied to diagnosis and therapeutics of carcinomas [1]. Therefore, the study of analytical methods for determination of tetracyclines has been a noticeable project in the field of drug analysis [2][3][4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Ion Selective PVC Membrane Electrode for the Determination of Methacycline Hydrochloride in Pharmaceutical Formulation

Aboul‐Enein

Sun

2002

Sensors

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A plastic membrane electrode for the determination of methacycline hydrochloride was fabricated based on the use of methacycline-tetraphenylborate as the electroactive substance, and dioctylphtalate as the plasticizing agent. It was found that the potential responses of the electrode were influenced by pH of the tested solution when pH>3. Under the condition of pH 2.6, the linear response range, slope (25ºC) and detection limit obtained were 3.0×10 -2~6.0×10-6 M, 52.9mv per decade and 3.4×10 -6 M ,respectively.The response time of the electrodes was <15 seconds. The values of recovery obtained were 99.2~101.2%. The electrode was successfully applied to determination of methacycline hydrochloride in tablet by direct potentiometric method. The result obtained with the electrode was in good agreement with the value obtained by using the official method (highperformance liquid chromatography).

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Liquid chromatographic separation of doxycycline and 4-epidoxycycline in a tissue depletion study of doxycycline in turkeys

Cited by 46 publications

References 11 publications

Effect of doxycycline concentrations in chicken tissues as a consequence of permanent exposure to enrofloxacin traces in drinking water

Effect of doxycycline concentrations in chicken tissues as a consequence of permanent exposure to enrofloxacin traces in drinking water

LC-MS/MS analysis of doxycycline residues in chicken tissues after oral administration

Ion Selective PVC Membrane Electrode for the Determination of Methacycline Hydrochloride in Pharmaceutical Formulation

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