Liquid chromatographic separation of aminoglycosides with pulsed amperometric detection

Polta, John A.; Johnson, Dennic C.; Merkel, K.E.

doi:10.1016/s0021-9673(01)81339-x

Cited by 54 publications

(19 citation statements)

References 7 publications

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“…The majority of applications utilizing the triple-step PAD waveform execute Mode I detections. Such detections are typically performed at Au electrodes under alkaline conditions and Pt electrodes under both alkaline and acidic conditions for carbohydrates, alditols, aminoglycosides, and alcohols [15][16][17][18].…”

Section: Historical Developments Of Pedmentioning

confidence: 99%

A review of pulsed electrochemical detection following liquid chromatography and capillary electrophoresis

Fedorowski

LaCourse

2015

Analytica Chimica Acta

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Section: Historical Developments Of Pedmentioning

confidence: 99%

A review of pulsed electrochemical detection following liquid chromatography and capillary electrophoresis

Fedorowski

LaCourse

2015

Analytica Chimica Acta

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“…In addition to the UV detection or fluorescence detection obtained by derivatization, other types of detection may be employed in the determination of aminoglycosides, such as amperometric detection (Megoulas, Koupparis 2005), evaporative light scattering detection (Serrano, Silva, 2006;Lovering et al, 1984) and mass spectrometry (Bogialli et al, 2005). Despite the advantages and popularization of employment of ion-pair reversed phase high performance liquid chromatography (HPLC), there are other methods that use ion-exchange chromatography with chemiluminescent detection (Polta et al, 1985). In addition the HPLC, spectrophotometry may also be employed in the determination of apramycin, tobramycin and kanamycin in pharmaceutical dosages (Bontchev et al, 1984).…”

Section: Introductionmentioning

confidence: 99%

Antibiotic microbial assay using kinetic-reading microplate system

Lourenço

Pinto

2011

Braz. J. Pharm. Sci.

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The aim of this study was to determine the optimal experimental conditions to develop a methodology for microbiological assay of apramycin employing microplate and kinetic reading mode, and to validate the developed method, through evaluation of parameters of selectivity, linearity, linear range, limits of detection and quantification, accuracy and precision. The turbidimetric assay principle is simple: the test solution is added to a suspension of test microorganism in culture media, the mixture is incubated under appropriate conditions and the microbial growth is measured by photometric reading. Microplate with kinetic reading mode employed in antibiotic assay is of considerable interest since it allows reduction of material and analysis time and enables a large number of samples to be analyzed simultaneously, with automated reading and calculating. Established conditions considered the standard-curve of apramycin at concentrations from 5.0 to 35.0 mg mL -1 , and tryptic soy broth inoculated with 5% Escherichia coli (ATCC 8739) suspension. Satisfactory results were obtained with 2 hours of incubation. The developed method showed appropriate selectivity, linearity in the range from 5.0 to 35.0 mg mL , respectively, as well as satisfactory accuracy (recuperation = 98.5%) and precision (RSD = 6.0%). Microplate assay combined the characteristics of microbiological (evaluation of antibiotic activity against sensitive test microorganism) and physico-chemical (operationally straightforward and faster results) assays.Uniterms: Antibiotics/microbial assay. Apramycin/microbial assay. Apramycin sulfate. Turbidimetric assay/medicines analysis.O objetivo deste trabalho é determinar as condições experimentais ideais para o desenvolvimento de metodologia para a dosagem microbiológica de apramicina empregando microplacas e modo de leitura cinético e validar o método desenvolvido, através da avaliação dos parâmetros de especificidade e seletividade, linearidade, faixa ou intervalo linear, limite de detecção e quantificação, exatidão e precisão. O ensaio turbidimétrico é simples: a solução-teste é adicionada à suspensão de microrganismo-teste em meio de cultura, a mistura é incubada em condições apropriadas e o crescimento microbiano é medido por meio de leitura fotométrica. O emprego de método de microplacas com leitura cínética para a dosagem de antibióticos é de interesse considerável, uma vez que possibilita reduzir quantidade de material e tempo de análise necessários e permite o ensaio de grande número de amostras simultaneamente, com leitura e cálculo automatizados. As condições estabelecidas abrangem curva-padrão de apramicina com concentrações entre 5 e 35 mg/mL, e emprego de meio de cultura caldo de triptona-soja inoculado com Escherichia coli (ATCC 8739) na proporção de 5%. Foram obtidos resultados satisfatórios após 2 horas de incubação. O método desenvolvido apresentou especificidade e seletividade adequadas, linearidade na faixa de 5 a 35 mg/mL, limite de detecção e quantificação de 0,1 e 0,4 mg/mL, respectivament...

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“…Finally, the potential is stepped to a negative value (E red ) to reduce the oxide layer formed at E oxd , thereby regenerating the electrode activity so that the cycle may be repeated. Aminoglycoside antibiotics were first determined by pulsed amperometric detection at a platinum electrode by Polta and Johnson [8]. Statler extended this work by anion-exchange chromatography followed by pulsed amperometric detection at Au electrode under alkaline conditions [9].…”

Section: Introductionmentioning

confidence: 99%

Characterization and photocatalytic activity of transition-metal-supported surface bond-conjugated TiO2/SiO2

Tang

2004

Catalysis Today

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Liquid chromatographic separation of aminoglycosides with pulsed amperometric detection

Cited by 54 publications

References 7 publications

A review of pulsed electrochemical detection following liquid chromatography and capillary electrophoresis

A review of pulsed electrochemical detection following liquid chromatography and capillary electrophoresis

Antibiotic microbial assay using kinetic-reading microplate system

Characterization and photocatalytic activity of transition-metal-supported surface bond-conjugated TiO2/SiO2

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