Liquid chromatographic separation of amino acids, peptides, and derivatives on a porous polystyrene-divinylbenzene copolymer

Iskandarani, Ziad; Pietrzyk, Donald J.

doi:10.1021/ac00226a025

Cited by 63 publications

(6 citation statements)

References 26 publications

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“…Exposure of silica to pH values greater than 8 or lower than 2 starts to degrade its matrix (Glajch et al 1987). If peptide separation conditions are favored at pH above 8, a stationary phase made of polystyrene/ divinylbenzene particles should be used (Iskandarani and Pietrzyk 1981). Takenaka et al (2000) used reversed-phase chromatography to isolate enterostatin derived from digested proglycinin employing as stationary phases octadecyl silica and phenethyl silica.…”

Section: Reversed-phase Chromatographymentioning

confidence: 99%

Production and purification of recombinant hypocholesterolemic peptides

et al. 2014

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Elevated concentrations of cholesterol in plasma are associated with increased risk for heart diseases in humans. Bioactive peptides can be considered as an option to prevent or treat this condition. Currently, there are wide sources of bioactive peptides with hypocholesterolemic activities; however, most researches are focused in bioactive peptides derived from soybean and milk protein. Although there are several preparation methods for these peptides, it is a novel process to prepare bioactive peptides by genetic engineering techniques. In this review, after a general introduction on approaches and advances in bioactive peptides, recombinant strategies to generate hypocholesterolemic peptides and their purification are discussed as well as their application in food and drug design.

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Section: Reversed-phase Chromatographymentioning

confidence: 99%

Production and purification of recombinant hypocholesterolemic peptides

et al. 2014

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“…Besides the presence of the ionizable carboxylic acid group of the amino acid moiety, the dimethylamino group of the dansyl moiety is a weak base with pK a between 3.0 and 4.0, and this value is largely independent of the ionic properties of the side chain of the amino acid [29,30]. These two ionizable functional groups explain why Dns-AA behaved as typical ampholytes without zwitterionic properties in reversed-phase HPLC [31], with the exception of the three basic amino acids, Dns-His, Dns-Arg and Dns-Lys, which are zwitterionic. In addition, Dns-AA possess in their structures a nonpolar grouping, which is the dansyl portion.…”

Section: Ionizable Speciesmentioning

confidence: 99%

Capillary electrochromatography with monolithic stationary phases: 1. Preparation of sulfonated stearyl acrylate monoliths and their electrochromatographic characterization with neutral and charged solutes

2002

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A novel monolithic stationary phase having long alkyl chain ligands (C17) was introduced and evaluated in capillary electrochromatography (CEC) of small neutral and charged species. The monolithic stationary phase was prepared by the in situ copolymerization of pentaerythritol diacrylate monostearate (PEDAS) and 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPS) in a ternary porogenic solvent consisting of cyclohexanol/ethylene-glycol/water. While AMPS was meant to support the electroosmotic flow (EOF) necessary for transporting the mobile phase through the monolithic capillary, the PEDAS was introduced to provide the nonpolar sites for chromatographic retention. Monolithic columns at various EOF velocities were readily prepared by conveniently adjusting the amount of AMPS in the polymerization solution as well as the composition of the porogenic solvent. The monolithic stationary phases thus obtained exhibited reversed-phase chromatography behavior toward neutral solutes and yielded a relatively strong EOF. For charged solutes (e.g., dansyl amino acids), nonpolar as well as electrostatic interaction/repulsion with the monoliths were observed in addition to electrophoretic migration. Therefore, for charged solutes, selectivity and migration can be readily manipulated by changing various parameters including the nature of the monolith and the composition of the mobile phase (e.g., pH, ionic strength and organic modifier). Ultrafast separation on the time scale of seconds of 17 different charged and neutral pesticides and metabolites were performed using short capillary columns of 8.5 cm x 100 microm ID.

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“…The inclusion of adsorption and chromatographic steps within the flowsheet of a biotechnology process requires the development of high-capacity and -selectivity adsorbents, allowing the operation to run at the mildest possible conditions so that the stability of the target compound is not compromised and the economic viability of the process is achieved. Because of their adequate physical properties, adsorption capacity, and relatively low cost, the polymeric divinylbenzene−styrene (DVB−S) resins are employed by the food and pharmaceutical industries for the purification of amino acids and peptides, antibiotics such as thienamicin, vitamin B 12 , prostaglandine, etc. Moreover, the chemically modified adsorbents of this type have shown to exhibit significant improvements in the capacity and selectivity for some compounds such as cephalosporin C, amino acids, and peptides …”

Section: Introductionmentioning

confidence: 99%

Adsorption Isotherms of Aspartame on Commercial and Chemically Modified Divinylbenzene−Styrene Resins at Different Temperatures

et al. 2002

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The equilibria of adsorption of aspartame in an aqueous solution on a commercial resin and three chemically modified Amberlite XAD-2 resins were measured at different temperatures within the range (0 to 35) °C. The functional groups, introduced by nucleophilic substitution in the aromatic rings of the divinylbenzene−styrene matrix, were bromine (−Br), bromoethyl (−CH2CH2Br), and chloromethyl (−CH2Cl). All of the isotherms showed a nonlinear and favorable shape with decreasing adsorption capacity as the temperature increased. The chloromethylated resin, and, to a lesser degree, the bromoethylated resin, increased the adsorption capacity for aspartame compared to Amberlite XAD-2. At 35 °C, the chloromethyl resin showed up to a 280% higher saturation capacity than the commercial adsorbent. The experimental equilibrium data were fitted to the Langmuir, Freundlich, Langmuir−Freundlich, Redlich−Peterson, and Toth models. Toth and Langmuir−Freundlich isotherms provided very good fittings for all of the resins over the temperature range studied, whereas the Langmuir and Redlich−Peterson equations were less accurate, although the average errors were, in general, below 10% with respect to the measured values.

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Liquid chromatographic separation of amino acids, peptides, and derivatives on a porous polystyrene-divinylbenzene copolymer

Cited by 63 publications

References 26 publications

Production and purification of recombinant hypocholesterolemic peptides

Production and purification of recombinant hypocholesterolemic peptides

Capillary electrochromatography with monolithic stationary phases: 1. Preparation of sulfonated stearyl acrylate monoliths and their electrochromatographic characterization with neutral and charged solutes

Adsorption Isotherms of Aspartame on Commercial and Chemically Modified Divinylbenzene−Styrene Resins at Different Temperatures

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