Liquid chromatographic method for quantifying polyphenols in ciders by direct injection

Fernández‐Suárez, Belén; Palacios, Noemí; Fraga, Natalia; Rodríguez, Roberto Oropesa

doi:10.1016/j.chroma.2005.01.022

Cited by 41 publications

(35 citation statements)

References 39 publications

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“…HPLC analysis was performed according to a previously described method (Suárez et al 2005) using a Waters system equipped with a 717 automatic injector, a column oven, two pumps (model 510), a diode array detector (model 2996) and the Empower 2 software data module. Separation of polyphenols was carried out on a reversed-phase Nucleosil 120 C 18 (250 Â 4.6 mm I.D, 3 mm) column (Teknokroma, Barcelona, Spain) at 30 1C, using a flow rate of 0.8 ml/min.…”

Section: Reversed-phase High Performance Liquid Chromatography (Hplc)mentioning

confidence: 99%

In vitro anti-herpetic activity of an aqueous extract from the plant Phyllanthus orbicularis

et al. 2009

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The Herpesviridae includes at least eight viral species pathogenic for humans, responsible for a wide variety of clinical symptoms. The lack of an effective vaccine and the moderate to high toxicity of the available synthetic antiherpes compounds emphasises the need for new inhibitors. Several Phyllanthus genus (Euphorbiaceae) members have been widely used in traditional medicine and their biological properties have been intensely studied. In this study we investigated the in vitro antiviral activity of the Cuban-endemic plant Phyllanthus orbicularis H.B.K. against Herpes simplex virus type 1 (HSV-1) and 2 (HSV-2) reference strains and clinical isolates with different sensitivities to acyclovir. The inhibitory activity on Human cytomegalovirus (HCMV) replication was also investigated. The selectivity indexes (SI) found for Ph. orbicularis aqueous extract ranged from 8.7 to 37.6. Studies on the antiviral mechanisms involved revealed that the drug acted at early stages of herpesvirus replication, possibly by producing a virucidal effect, although further inhibition of intracellular replication events could not be ruled out.

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Section: Reversed-phase High Performance Liquid Chromatography (Hplc)mentioning

confidence: 99%

In vitro anti-herpetic activity of an aqueous extract from the plant Phyllanthus orbicularis

et al. 2009

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“…These methods usually involve a chromatographic separation using RP-LC with conventional C 18 columns and, some of them, a previous SPE step to remove or minimize interferences from sample matrix [9,10,14]. More recently, it has been reported that the determination of phenolics in ciders is done by direct injection [17] using conventional C 18 columns.…”

Section: Introductionmentioning

confidence: 99%

Determination of some hydroxybenzoic acids and catechins in white wine samples by liquid chromatography with luminescence detection

2006

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A liquid chromatographic method with luminescence detection for the determination of eight phenolic compounds is reported. The method involves postcolumn derivatization with terbium(III). This derivatization is based on the reaction between phenolics and terbium(III) to form luminescent chelates, which were determined at lamda ex 295 and lamda em 545 nm using the fluorescence mode. The long wavelength emission of lanthanide chelates can minimize interferences from background sample matrix, which usually emit at shorter wavelengths. Also, the chromatographic separation allows the individual determination of phenolics, which cannot be done using the direct measurement of the fluorescence of their corresponding terbium chelates. Dynamic ranges of the calibration graphs and detection limits, obtained with standard solutions of analytes were (microg/mL): gallic acid (0.9-40, 0.3), protocatechuic acid (0.05-7, 0.016), catechin (0.2-40, 0.07), vanillic acid (0.25-40, 0.08), p-hydroxybenzoic acid (0.8-40, 0.25), syringic acid (0.17-40, 0.05), epicatechin (0.3-40, 0.09) and salicylic acid (0.07-12, 0.02). The precision was established at two concentration levels of each analyte and expressed as the percentage of RSD with values ranging between 1.0 and 6.5%. The practical usefulness of the method was demonstrated by the analysis of white wine samples, which were diluted two-fold and directly injected into the chromatographic system. The recovery values obtained ranged between 93.3 and 108.0%.

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“…HPLC analysis was performed according to the method validated by our group (Suárez, Palacios, Fraga, & Rodríguez, 2005). We used a Waters system equipped with a 717 automatic injector, provided with a column oven, two pumps (model 510), a diode array detector (model 2996) and Millennium software v.3.1 data module.…”

Section: Liquid Chromatographic Analysis Of Phenolic Compoundsmentioning

confidence: 99%

Phenolic profiles, antioxidant activity and in vitro antiviral properties of apple pomace

et al. 2010

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a b s t r a c tMethanolic and acetonic extracts of apple pomace were evaluated for phenolic profiles, antioxidant properties and antiviral effect against herpes simplex virus type 1 (HSV-1) and 2 (HSV-2). Acetone extraction yielded the higher amounts of phenolic compounds. The extraction method influenced the phenolic composition although antioxidant activity correlated weakly with phenols concentration. Among the polyphenols analysed, quercetin glycosides were the most important family, followed by dihydrochalcones. Apple pomace extracts were able to inhibit both HSV-1 and HSV-2 replication in Vero cells by more than 50%, at non-cytotoxic concentrations. Selectivity indexes (SI) ranged from 9.5 to 12.2.

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Liquid chromatographic method for quantifying polyphenols in ciders by direct injection

Cited by 41 publications

References 39 publications

In vitro anti-herpetic activity of an aqueous extract from the plant Phyllanthus orbicularis

In vitro anti-herpetic activity of an aqueous extract from the plant Phyllanthus orbicularis

Determination of some hydroxybenzoic acids and catechins in white wine samples by liquid chromatography with luminescence detection

Phenolic profiles, antioxidant activity and in vitro antiviral properties of apple pomace

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