Liquid chromatographic‐mass spectrometric analysis of conjugated diene fatty acids in a partially hydrogenated fat

Banni, Sebastiano; Day, Billy W.; Evans, Rhobert W.; Corongiu, Francesco P.; Lombardi, Benito

doi:10.1007/bf02541348

Cited by 73 publications

(43 citation statements)

References 10 publications

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“…Calcium salts of PHVO (100 to 400 g/day) have also been fed and milk fat yield was modestly reduced (5% to 15%) in one study (Piperova et al, 2004) and unaffected in another (Selberg et al, 2004). The observed milk fat decrease from PHVO has routinely been attributed to trans-18:1 FA; however, trans-C18:1 represent only about 40% to 60% of the total FA in PHVO, and many other unusual FA isomers, including CLAs, are formed in the production of PHVO (Banni et al, 1994;Jung and Ha, 1999). Thus, when one considers the potency of trans-10, cis-12 CLA, it is clearly possible that the PHVOinduced reduction in milk fat could be due to small amounts of unique FA other than the trans-C18:1 isomers.…”

Section: Biohydrogenation Intermediatesmentioning

confidence: 95%

Recent advances in the regulation of milk fat synthesis

Harvatine

Boisclair

Bauman

2009

Animal

264

272

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In addition to its economic value, milk fat is responsible for many of milk's characteristics and can be markedly affected by diet. Diet-induced milk fat depression (MFD) was first described over a century ago and remains a common problem observed under both intensive and extensive management. The biohydrogenation theory established that MFD is caused by an inhibition of mammary synthesis of milk fat by specific fatty acids (FA) produced as intermediates in ruminal biohydrogenation. During MFD, lipogenic capacity and transcription of key lipid synthesis genes in the mammary gland are down-regulated in a coordinated manner. Our investigations have established that expressions of sterol response element-binding protein 1 (SREBP1) and SREBP-activation proteins are down-regulated during MFD. Importantly, key lipogenic enzymes are transcriptionally regulated via SREBP1. Collectively, these results provide strong evidence for SREBP1 as a central signaling pathway in the regulation of mammary FA synthesis. Spot 14 is also down-regulated during MFD, consistent with a lipogenic role for this novel nuclear protein. In addition, SREBP1c and Spot 14 knock-out mice exhibit reduced milk fat similar to the magnitude and pattern of MFD in the cow. Application of molecular biology approaches has provided the latest chapter in the regulation of milk fat synthesis and is reviewed along with a brief background in nutritional regulation of milk fat synthesis in ruminants.

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Section: Biohydrogenation Intermediatesmentioning

confidence: 95%

Recent advances in the regulation of milk fat synthesis

Harvatine

Boisclair

Bauman

2009

Animal

264

272

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“…Other fatty acids used were purchased from Sigma Chemical Co. (St. Louis, MO). They were stored in ethyl alcohol at Ϫ 22 Њ C. Contents of solutions were determined monthly by capillary gas liquid chromatography of fatty acids as methyl esters prepared using the methanol-boron trifluoride method (19), specifically modified for CLA (20). [1-14 C] cis -9, cis -12-C 18:2 (linoleic acid, LA; 60.8 Ci/mol) was from NEN Life Sciences Products (Boston, MA).…”

Section: Methodsmentioning

confidence: 99%

Conjugated linoleic acid isomers in mitochondria

Demizieux

Degrace

Gresti

et al. 2002

Journal of Lipid Research

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The beneficial effects exerted by low amounts of conjugated linoleic acids (CLA) suggest that CLA are maximally conserved and raise the question about their mitochondrial oxidizability. Cis -9, trans -11-C 18:2 (CLA1) and trans -10, cis -12-C 18:2 (CLA2) were compared to cis -9, cis -12-C 18:2 (linoleic acid; LA) and cis -9-C 16:1 (palmitoleic acid; PA), as substrates for total fatty acid (FA) oxidation and for the enzymatic steps required for the entry of FA into rat liver mitochondria. Oxygen consumption rate was lowest when CLA1 was used as a substrate with that on CLA2 being intermediate between it and the respiration on LA and PA. The order of the radiolabeled FA oxidation rate was PA ϾϾ LA Ͼ CLA2 Ͼ CLA1. Transesterification to acylcarnitines of the octadecadienoic acids were similar, while uptake across inner membranes of CLA1 and, to a lesser extent, of CLA2 was greater than that of LA or PA. Prior oxidation of CLA1 or CLA2 made re-isolated mitochondria much less capable of oxidising PA or LA under carnitine-dependent conditions, but without altering the carnitine-independent oxidation of octanoic acid. Therefore, the CLA studied appeared to be both poorly oxidizable and capable of interfering with the oxidation of usual FA at a step close to the beginning of the ␤ -oxidative cycle. -Demizieux, L., P. Degrace, J. Gresti, O. Loreau, J-P. Noël, J-M. Chardigny, J-L. Sébédio, and P. Clouet. Conjugated linoleic acid isomers in mitochondria: evidence for an alteration of fatty acid oxidation.

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“…Vegetable oils contain a very low amount ranging from 0.1 mg CLA g −1 oil for coconut oil to 0.7 mg CLA g −1 oil for safflower oil (Chin et al, 1992). Banni et al (1994) carried out series of HPLC/UV/MS analyses to characterise the fatty acids with conjugated dienes in partially hydrogenated oil and they detected a low quantity of CLA isomers (4.24 mg total CLA g −1 oil). CLA isomers are industrially produced by alkaline conjugated isomerisation of linoleic acid from safflower or sunflower oil with the total triglycerides hydrolysis (Berdeaux et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Conjugated linoleic acid formation by hydrogenation/isomerisation of safflower oil over bifunctional structured catalyst Rh/SBA‐15

et al. 2011

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Directed isomerisation of safflower oil under very low hydrogen partial pressure of 7 psi over a novel bifunctional highly structured rhodium-based catalyst (Rh/SBA-15), having narrow pore size distribution ranging from 4 to 8 nm, and BET-specific surface of ≈1,000 m 2 g −1 , was investigated as a new chemocatalytic approach for vegetable oil hardening and simultaneously producing health-beneficial conjugated linoleic acids (CLA). Time course profiles of (cis-9, trans-11)-; (cis-10, trans-12)-; (trans-10, cis-12)-; (cis,cis)-and (trans, trans)-octadecadienoic isomers (CLAs) as well as the other fatty acids traditionally encountered during the hydrogenation of vegetable oils are presented and discussed under selected process conditions. Preliminary results show that it is possible to tailor characteristics of the hydrogenation catalyst in such way to confer its bi-functional activity: hydrogenation and conjugation isomerisation.

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Liquid chromatographic‐mass spectrometric analysis of conjugated diene fatty acids in a partially hydrogenated fat

Cited by 73 publications

References 10 publications

Recent advances in the regulation of milk fat synthesis

Recent advances in the regulation of milk fat synthesis

Conjugated linoleic acid isomers in mitochondria

Conjugated linoleic acid formation by hydrogenation/isomerisation of safflower oil over bifunctional structured catalyst Rh/SBA‐15

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