Liquid chromatographic estimation of tranylcypromine in human plasma

Dagneaux, P. G. L. C. Krugers; Loohuis, C. P. G. G.; Elhorst, J.T.Klein; Veer, T. S. Van der

doi:10.1007/bf01980481

Pharmaceutisch Weekblad Scientific Edition

1992

DOI: 10.1007/bf01980481

|View full text |Cite

Liquid chromatographic estimation of tranylcypromine in human plasma

P. G. L. C. Krugers Dagneaux

C. P. G. G. Loohuis

J.T.Klein Elhorst

et al.

Abstract: A simple, selective and quantitative estimation of tranylcypromine using liquid chromatography and electrochemical detection with apomorphine as internal standard in human plasma is described. Sample preparation is done by liquid-liquid extraction. The absolute mean recovery of apomorphine is 95%, the recovery of tranylcypromine is 81%. This method gives a simple estimation method to assay plasma concentrations of tranylcypromine in the therapeutic range (5-200 ng/ml) with good reproducibility and linearity.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

1993

1996

Publication Types

Select...

Article3

Relationship

Self Cite0

Independent3

Authors

Journals

Cited by 3 publications

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Direct separation of tranylcypromine enantiomers and their profile in an atypical depressive patient

Aboul‐Enein

Serignese

1995

Biomedical Chromatography

View full text Add to dashboard Cite

An isocratic and simple high-performance liquid chromatographic method is developed for the direct resolution of the tranylcypromine (TCP) enantiomers. The method involves the use of an S-18-crown-6-ether chiral stationary phase known as the Crownpak CR (+) column. The stereochemical separation factor (alpha) obtained was 1.30 and the stereochemical resolution factor (Rs) was 0.69 when using a mobile phase consisting of 0.1 N perchloric acid containing 12% methanol at 23 degrees C. The method has been used to monitor and identify, qualitatively, the profile of enantiomers of TCP in urine of an atypical depressive patient. It was found that the (-)-S-TCP concentration is significantly higher than the (+)-R-enantiomer.

show abstract

Direct separation of tranylcypromine enantiomers and their profile in an atypical depressive patient

Aboul‐Enein

Serignese

1995

Biomedical Chromatography

View full text Add to dashboard Cite

show abstract

In vitro and in vivo irreversible plasma protein binding of beclobric acid enantiomers

Mayer¹,

Mutschler²,

Spahn‐Langguth³

et al. 1993

Chirality

View full text Add to dashboard Cite

Acyl glucuronides are known to be labile conjugates, which undergo hydrolysis and bind irreversibly to proteins. The lipid-regulating agent (+/-)-beclobrate is immediately converted to the free acid after oral administration. Further metabolism leads to formation of the corresponding diastereomeric acyl glucuronides. Beclobric acid glucuronides were quantified by indirect measurement with an HPLC method based on chiral fluorescent derivatization of the carboxylic acid and subsequent normal-phase chromatography. The renal clearance of unchanged drug is low, with almost all drug excreted into urine as glucuronic acid conjugates. Beclobric acid glucuronide is also detectable in plasma. In vitro degradation studies with beclobric acid glucuronide (at a concentration of 5 microM in 150 mM phosphate buffer pH 7.4) exhibited a minor tendency for acyl migration and hydrolysis, i.e., a higher stability than has been observed for the acyl glucuronides of most other drugs. The in vitro degradation half-lives of the two beclobric acid beta-1-O-acyl glucuronides were 22.7 and 25.7 h. After incubation with pooled plasma and human serum albumin in buffer pH 7.4 irreversible binding was measured in vitro. No significant difference between the two enantiomers was detected with respect to the magnitude of in vitro irreversible binding. In 3 healthy male volunteers the extent of irreversible binding of both beclobric acid enantiomers to plasma proteins was investigated after single and multiple oral doses of racemic beclobrate (100 mg once daily). Irreversible binding of both enantiomers was observed in all volunteers. The adduct densities for (-)- and (+)-beclobric acid after single 100 mg beclobrate doses were 0.147 x 10(-4) and 0.177 x 10(-4) mol/mol protein. Multiple dosing increased irreversible binding 3- to 4-fold.

show abstract

Determination of Tranylcypromine in Urine and Pharmaceutical Formulation by HPLC Using Symmetry column

Aboul‐Enein

Abou-Basha

1996

Journal of Liquid Chromatography & Related Technologies

View full text Add to dashboard Cite

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

hi@scite.ai

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Liquid chromatographic estimation of tranylcypromine in human plasma

Cited by 3 publications

References 12 publications

Direct separation of tranylcypromine enantiomers and their profile in an atypical depressive patient

Direct separation of tranylcypromine enantiomers and their profile in an atypical depressive patient

In vitro and in vivo irreversible plasma protein binding of beclobric acid enantiomers

Determination of Tranylcypromine in Urine and Pharmaceutical Formulation by HPLC Using Symmetry column

Contact Info

Product

Resources

About