Liquid chromatographic–electrospray ionization mass spectrometric quantitative analysis of buprenorphine, norbuprenorphine, nordiazepam and oxazepam in rat plasma

Pirnay, Stéphane; Hervé, Françoise; Bouchonnet, Stéphane; Perrin, Bérengère; Baud, Frédéric J.; Ricordel, I.

doi:10.1016/j.jpba.2006.02.020

Cited by 22 publications

(18 citation statements)

References 38 publications

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“…In order to test the in vivo applicability of the described procedure, a 0.5 mL intraperitoneal injection (IP) of buprenorphine (30 mg/kg by dissolving 15 mg of buprenorphine in 1 mL of sterile water and 25% cyclodextrine) was carried out on a female Wistar rat (250 g). The IP concentration was chosen based on the administrated dose generally found in the literature 31, 32. Two 5 μL DBS samples were collected at different times after administration ( i.e .…”

Section: Methodsmentioning

confidence: 99%

On‐line desorption of dried blood spots coupled to hydrophilic interaction/reversed‐phase LC/MS/MS system for the simultaneous analysis of drugs and their polar metabolites

Thomas

Déglon

Steimer

et al. 2010

J of Separation Science

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An assay for the simultaneous analysis of pharmaceutical compounds and their metabolites from micro-whole blood samples (i.e. 5 microL) was developed using an on-line dried blood spot (on-line DBS) device coupled with hydrophilic interaction/reversed-phase (HILIC/RP) LC/MS/MS. Filter paper is directly integrated to the LC device using a homemade inox desorption cell. Without any sample pretreatment, analytes are desorbed from the paper towards an automated system of valves linking a zwitterionic-HILIC column to an RP C18 column. In the same run, the polar fraction is separated by the zwitterionic-HILIC column while the non-polar fraction is eluted on the RP C18. Both fractions are detected by IT-MS operating in full scan mode for the survey scan and in product ion mode for the dependant scan using an ESI source. The procedure was evaluated by the simultaneous qualitative analysis of four probes and their relative phase I and II metabolites spiked in whole blood. In addition, the method was successfully applied to the in vivo monitoring of buprenorphine metabolism after the administration of an intraperitoneal injection of 30 mg/kg on adult female Wistar rat.

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Section: Methodsmentioning

confidence: 99%

On‐line desorption of dried blood spots coupled to hydrophilic interaction/reversed‐phase LC/MS/MS system for the simultaneous analysis of drugs and their polar metabolites

Thomas

Déglon

Steimer

et al. 2010

J of Separation Science

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“…[1][2][3] Benzodiazepines bind to a non-specific site of the γ-aminobutyric acid (GABA) receptor, leading to increased GABA affinity to the receptor site. [4,10,11] Various methods for determination of benzodiazepines and zolpidem from pharmaceutical formulations and biological samples from living or deceased abusers, have been reported including high-performance liquid chromatography (HPLC), [12] gas chromatography (GC), [13] gas chromatography-mass spectrometry (GC-MS), [14,15] gas chromatography-tandem mass spectroscopy (GC-MS/MS), [16] liquid chromatography-mass spectrometry (LC-MS), [17,18] and liquid chromatography-tandem mass spectrometry (LC-MS/MS). [4] Zolpidem is a non-benzodiazepine hypnotic having similar action with 1,4-benzodiazepines and belongs to imidazopyridine class.…”

Section: Introductionmentioning

confidence: 99%

“…[5][6][7][8][9] Illicit use and misuse of benzodiazepine drugs is related to sexual assault and robbery crimes, suicides, and reducing after-effects of LSD, amphetamine or Ecstasy. [4,10,11] Various methods for determination of benzodiazepines and zolpidem from pharmaceutical formulations and biological samples from living or deceased abusers, have been reported including high-performance liquid chromatography (HPLC), [12] gas chromatography (GC), [13] gas chromatography-mass spectrometry (GC-MS), [14,15] gas chromatography-tandem mass spectroscopy (GC-MS/MS), [16] liquid chromatography-mass spectrometry (LC-MS), [17,18] and liquid chromatography-tandem mass spectrometry (LC-MS/MS). [19][20][21] Concerning the ionization mode of 1,4-benzodiazepines in LC-MS, the large majority of literature data indicates positive electrospray ionization (ESI) as most suitable for these compounds.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous ESI‐APCI(+) ionization and fragmentation pathways for nine benzodiazepines and zolpidem using single quadrupole LC‐MS

Galaon

Vacaresteanu

Anghel

et al. 2013

Drug Testing and Analysis

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Nine important 1,4-benzodiazepines and zolpidem were characterized by liquid chromatography-mass spectrometry using a multimode ionization source able to generate ions using both electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI), and a single quadrupole mass analyzer. An optimum chromatographic separation was applied for all target compounds in less than 8 minutes using a Zorbax Eclipse Plus column (100 x 4.6 mm, 3.5 μm) kept at 35°C and a 0.3% HCOOH/ ACN/IPA (61:34:5) mobile phase pumped at 1 ml/min. Optimization of LC-MS method generated low limit of quantitation (LOQ) values situated in the range 0.3-20.5 ng/ml. Comparison between differences in method sensitivity, under specified chromatographic conditions, when using ESI-only, APCI-only, and simultaneous ESI-APCI ionization with such a multimode source was discussed. Mixed ESI-APCI(+) mode proved to be the most sensitive ionization generating an average 35% detector response increase compared to ESI-only ionization and 350% detector response increase with respect to APCI-only ionization. Characterization of the nine benzodiazepines and zolpidem concerning their MS fragmentation pathway following 'in-source' collision-induced dissociation is discussed in detail and some general trends regarding these fragmentations are set.

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“…1,2 As an analgesic, BP is 25-40 times more potent than morphine 3 and has been used successfully by intramuscular, intravenous or sublingual routes for the treatment of moderate to severe pain at dose range from 0.3 to 0.6 mg and for the treatment of opioid dependence at dose of 2 to 32 mg. 4 However, sublingual formulation of BP is water-soluble tablet, so it may be diverted for illicit intravenous use. To avoid this circumstance, BP has now been co-formulated in a sublingual tablet with naloxone (NLX).…”

Section: Introductionmentioning

confidence: 99%

“…But many methods had no reported assay validation or incomplete assay validation, and could not perform simultaneous determination of BP, NBP and NLX in human plasma. 1,[3][4][5]9 A rapid and simultaneous method for determination of BP, NBP and NLX in human plasma by UPLC-MS/MS was established. The method optimized the parameters of SPE and other UPLC-MS/MS parameters, so that can get the good recoveries and little interference.…”

Section: Introductionmentioning

confidence: 99%

Development of a Rapid and Simultaneous Detection Method for Buprenorphine, Norbuprenorphine and Naloxone in Human Plasma Using Ultra‐high Performance Liquid Chromatography‐tandem Mass Spectrometer with Solid‐phase Extraction

Guo

2011

Chin. J. Chem.

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A simultaneous method was successfully established and validated for the separation and determination of buprenorphine (BP), its primary metabolite, nor-buprenorphine (NBP) and a proposed co-formulate, naloxone (NLX) in human plasma. The method used buprenorphine-d 4 (BP-D 4 ), nor-buprenorphine-d 3 (NBP-D 3 ), naltrexone (NTX) as internal standards (ISs). 100 µL of plasma sample fortified with the ISs was cleaned up by solid-phase extraction (SPE), and was then separated on a Waters Acquity TM BEH C 18 column with gradient elution using methanol and water (containing 0.2% formic) at a flow rate of 0.25 mL•min -1 . The mass spectrometer was used for detection and was operated in the positive electrospray ionization with multiple reaction monitoring (MRM) mode. The three compounds were effectively separated in 5 min. The linear ranges of the compounds were 0.1-25, 0.25-25 and 0.05-25 ng•mL -1 for BP, NBP and NLX, respectively, with r≥0.9935. The method had high sensitivity (the limits of detection were 0.02, 0.1 and 0.01 ng•mL -1 for BP, NBP and NLX, respectively) and high recoveries (≥97.6%). The result was shown to be linear and satisfactorily met current acceptance criteria for validation of bioanalytical method: intra and inter assay precisions within the required limits of ≤25% RSD. The LOQs fulfilled the LOQ requirements: precision≤25% RSD, and was fully validated according to the State Food and Drug Administration (SFDA) regulations. The results demonstrated that ultra-high performance liquid chromatographytandem mass spectrometer (UPLC-MS/MS) with SPE was a powerful detection tool and contributed to pharmaceutical analysis in biological matrices.

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Liquid chromatographic–electrospray ionization mass spectrometric quantitative analysis of buprenorphine, norbuprenorphine, nordiazepam and oxazepam in rat plasma

Cited by 22 publications

References 38 publications

On‐line desorption of dried blood spots coupled to hydrophilic interaction/reversed‐phase LC/MS/MS system for the simultaneous analysis of drugs and their polar metabolites

On‐line desorption of dried blood spots coupled to hydrophilic interaction/reversed‐phase LC/MS/MS system for the simultaneous analysis of drugs and their polar metabolites

Simultaneous ESI‐APCI(+) ionization and fragmentation pathways for nine benzodiazepines and zolpidem using single quadrupole LC‐MS

Development of a Rapid and Simultaneous Detection Method for Buprenorphine, Norbuprenorphine and Naloxone in Human Plasma Using Ultra‐high Performance Liquid Chromatography‐tandem Mass Spectrometer with Solid‐phase Extraction

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