Liquid Chromatographic Determination of Tocopherols and Tocotrienols in Margarine, Infant Foods, and Vegetables

Konings, Erik J M; Roomans, H.H.S.; Beljaars, Paul R

doi:10.1093/jaoac/79.4.902

Cited by 67 publications

(26 citation statements)

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“…In recent years, HPLC has overtaken GC as the method of choice for vitamin E determination because of the greater flexibility and convenience it offers in handling different sample matrices 2. For routine determination of vitamin E in foods and tissues, the normal procedure is first to liberate the vitamin from the sample matrix by saponification 126–130. Saponification procedures require prolonged heating of the sample in aqueous potassium hydroxide at elevated temperatures, usually 70 °C.…”

Section: Methodsmentioning

confidence: 99%

Vitamin E

Bramley

Elmadfa

Kafatos

et al. 2000

J. Sci. Food Agric.

484

219

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There is a growing body of evidence to suggest that the dietary intakes of vitamin E are insufficient to protect against the long‐term health risks associated with oxidative stress. Traditional plant breeding and food processing technologies have not concerned themselves with maximising the levels of the tocopherols in the diet, and supplementation is necessary both for nutritive reasons and for the protection of fat‐rich foods against oxidative rancidity. The paper reviews the potential for improving the tocopherol levels in the diet, particularly α‐tocopherol. Genetic technologies have already demonstrated the potential to enhance tocopherol levels by up‐regulation of the final steps in the biosynthetic pathway. Other strategies for the enhancement of the vitamin E content of plant foods are considered both from the perspective of improved bioavailability and the levels in processed foods. Finally some priorities for future research in the field are described. © 2000 Society of Chemical Industry

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Section: Methodsmentioning

confidence: 99%

Vitamin E

Bramley

Elmadfa

Kafatos

et al. 2000

J. Sci. Food Agric.

484

219

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“…The methods detailed in [35,36,37,38] were followed to assess the contents of ascorbic acid (AsA), glutathione (GSH), and α-tocopherol (α-TOC).…”

Section: Determination Of Non-enzymatic Antioxidants Contentsmentioning

confidence: 99%

Cyanobacteria and Glutathione Applications Improve Productivity, Nutrient Contents, and Antioxidant Systems of Salt-Stressed Soybean Plant

Zaki

Belal

Rady

2019

ILNS

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Salt stress restricts plant performance by disrupting various physio-biochemical processes like photosynthesis. Plants growing in saline substrates show deficiencies in absorption of some essential elements due to the presence of excessive sodium (Na+) in the rhizosphere, which antagonizes beneficial cations and causing toxicity in metabolism. Cyanobacteria (CB; a natural biofertilizer) play a fundamental role in building-up soil fertility, thus increasing plant performance. Glutathione (GSH) is a well-known antioxidant, which contributes to increase salt tolerance in the plant. This work was conducted as a pot experiment (sand culture) in 2017 to study the combined effect of CB, applied as seed inoculation, and GSH, applied as foliar spray, on growth, pods and seed yields, the contents of antioxidants, osmoprotectants, and nutrients, and the antioxidative enzymes activities of soybean (Glycine max L., cv. Giza 111) plants grown under saline conditions. At fourth leaf stage (21 days after sowing; DAS), CB-pretreated seedlings were supplemented with NaCl (150 mM) along with Hoagland′s nutrient solution, and at the same time seedlings were sprayed with 1 mM GSH. Samples were taken at 60 DAS to assess morphological, physio-biochemical and antioxidant defense systems attributes. Results showed that the integrative application of CB and GSH under saline conditions was effective in improving significantly the growth characteristics, yield components, photosynthetic efficiency (pigments contents and chlorophyll fluorescence), membrane stability index, relative water content, contents of soluble sugars, free proline, ascorbic acid, glutathione, α-tocopherol, and protein, and activities of superoxide dismutase, catalase, and guaiacol peroxidase. The contents of macronutrients (N, P, K+, and Ca2+) were also increased significantly in Glycine max plants compared to the stressed control. In contrast, Na+ content and electrolyte leakage were significantly reduced. Our results recommend using the combined CB (as seed inoculation) and GSH (as foliar spray) application for soybean plantss to grow well under saline conditions.

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“…Vitamin E is composed of a number of tocopherol analogues ( α ‐, β ‐, γ ‐, δ ‐) and their corresponding unsaturated tocotrienols. Clinical interest in antioxidant micronutrients, such as vitamin E, in several degenerative human health conditions12 and retinol deficiency as well as their relationship with abnormal retinoids metabolism, which is associated in neural degeneration with ageing and certain brain pathologies such as Alzheimer's diseases,13, 14 has been growing in recent years. Recent epidemiological and clinical studies have suggested that fat‐soluble vitamins A and E protect against coronary heart disease and that they may play a key role in the prevention of some cancers 15–17.…”

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confidence: 99%

Identification and determination of fat‐soluble vitamins and metabolites in human serum by liquid chromatography/triple quadrupole mass spectrometry with multiple reaction monitoring

Priego-Capote

Jiménez

Granados

et al. 2007

Rapid Comm Mass Spectrometry

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A method for determination of fat-soluble vitamins K(1), K(3), A, D(2), D(3) and E (as alpha- and delta-tocopherol) and metabolites 25-hydroxyvitamin D(2) and D(3) and 1,25-dihydroxyvitamin D(3) in human serum by liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) in positive mode is proposed. Highly selective identification of the target compounds in serum was confirmed by the most representative transitions from precursor ion to product ion. Quantitative MS/MS analysis was carried out by multiple reaction monitoring optimizing the most sensitive transition for each analyte in order to achieve low detection limits (from 0.012 to 0.3 ng/mL estimated with serum). The analysis was performed with 1 mL of serum, which was subjected to protein precipitation, liquid-liquid extraction to an organic phase, evaporation to dryness and reconstitution with methanol. The precision of the overall method ranged from 3.17-6.76% as intra-day variability and from 5.07-11.53% as inter-day variability. The method, validated by the standard addition method, provides complete information on the fat-soluble vitamins profile, which is of interest in clinical and metabolomics studies.

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Liquid Chromatographic Determination of Tocopherols and Tocotrienols in Margarine, Infant Foods, and Vegetables

Cited by 67 publications

References 0 publications

Vitamin E

Vitamin E

Cyanobacteria and Glutathione Applications Improve Productivity, Nutrient Contents, and Antioxidant Systems of Salt-Stressed Soybean Plant

Identification and determination of fat‐soluble vitamins and metabolites in human serum by liquid chromatography/triple quadrupole mass spectrometry with multiple reaction monitoring

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