Liquid chromatographic determination of the plasma concentrations of cefotaxime and desacetylcefotaxime in plasma of critically ill patients

Rosseel, M. T.; Vandewoude, Koenraad

doi:10.1016/j.jchromb.2004.08.040

Cited by 7 publications

(1 citation statement)

References 14 publications

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“…Many studies have shown that this is a parentally active antibiotic with a similar antibacterial range and βlactamase resistance to other parenteral cerebrospinal fluid of the third generation [6]. Several methods have been applied both in the fields of pharmacy and biological fluids to determination CTX: polarographic [7], voltammetric [8][9][10][11], RP-HPLC [12][13][14][15][16], spectrophotometry [17,18], chemiluminescence [19,20], capillary electrophoresis [21,22]. The chromatography of hydrophilic interactions (HILIC) is a liquid chromatography (LC) strategy that combines a polar, stationary phase, and a mobile phase with significant water content, combined with an increase of a lower polar solvent.…”

Section: Introductionmentioning

confidence: 99%

Development of a validated hydrophilic interaction chromatography method for the determination of cefotaxime in pharmaceutical preparations

Kzar¹,

Rasheed

Hassan

2021

Egypt. J. Chem.

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Cefotaxime is considered as one of the semi-synthetic, 3rd generation, cephalosporin antibiotics that have been utilized for treating a lot of infections resulting from different organisms. The technique of hydrophilic chromatography interaction (HILIC) has increased the accuracy and sensitivity concerning other methods, particularly spectrophotometer. The objective of this article was to introduce a simple method for the estimation of cefotaxime in pure and pharmaceutical injection forms and study the separation mechanism of cefotaxime. HILIC mode achieved excellent separation under chromatographic conditions on a HALO ® HILIC 2.7 column (100 mm x 2.1 mm I.D.) at 35 ° C with the following conditions: 10:90% acetate buffer (pH 5.5-40 mM): acetonitrile as eluent and wavelength of detection 254 nm. The proposed HILIC method exhibited high precision (RSD% < 0.5%), concentration ranges of 100-5500 ppb, and the lowest detection limit was 6.8167 ppb with a coefficient of determination of 0.9998 for cefotaxime. The findings of the method were used statistical tests compared to the British Pharmacopoeia protocol, which did not show any difference in accuracy among the methods

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Section: Introductionmentioning

confidence: 99%

Development of a validated hydrophilic interaction chromatography method for the determination of cefotaxime in pharmaceutical preparations

Kzar¹,

Rasheed

Hassan

2021

Egypt. J. Chem.

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Caffeine

2010

Analytical Methods for Therapeutic Drug Monitoring and Toxicology

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Cefotaxime pharmacokinetics after oral application in the form of 3α,7α-dihydroxy-12-keto-5β-cholanate microvesicles in rat

Goločorbin-Kon

Mikov

Arafat

et al. 2009

Eur. J. Drug Metabol. Pharmacokinet.

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The aim of ths study was to investigate the pharmacokinetics of cefotaxime sodium (CEF) pharmacokinetics after oral application in the form of sodium 3alpha,7alpha-dihydroxy-12-keto-5beta-cholanate (MKC) microvesicles (MV) in rat. Thirty Male Wister rats were divided into six groups (n=5 per group). Groups were treated orally with: (i) CEF (15 mg/kg) saline solution (15 mg/kg); (ii) CEF (15 mg/kg) saline solution with MKC (2 mg/kg); (iii) CEF saline solution mixed with blank microvesicles; (iv) CEF (15 mg/kg) encapsulated in microvesicles with saline solution; (v) CEF saline solution (15 mg/kg) mixed with blank MKC microvesicules; (vi) CEF (15 mg/kg) encapsulated in MKC microvesicules with saline solution. Data were analyzed using noncompartmental model. CEF oral bioavailability was increased twofold when coadministered with MKC and when encapsulated in microvesicles and ninefold when encapsulated in MKC microvesicles compared to the same CEF dose administered orally as saline solution. The increased bioavailability of CEF resulting from CEF encapsulation in microvesicules with MKC suggests that this formulation can extend the application of CEF from parenteral only to oral application.

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Liquid chromatographic determination of the plasma concentrations of cefotaxime and desacetylcefotaxime in plasma of critically ill patients

Cited by 7 publications

References 14 publications

Development of a validated hydrophilic interaction chromatography method for the determination of cefotaxime in pharmaceutical preparations

Development of a validated hydrophilic interaction chromatography method for the determination of cefotaxime in pharmaceutical preparations

Caffeine

Cefotaxime pharmacokinetics after oral application in the form of 3α,7α-dihydroxy-12-keto-5β-cholanate microvesicles in rat

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