Liquid Chromatographic Determination of Strychnine in Stomach Contents

Hoogenboom, J.J.L.; Rammell, C. G.

doi:10.1093/jaoac/68.6.1131

Cited by 4 publications

(6 citation statements)

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“…These results correspond with the concentrations found in other studies and suggest that strychnine is cleared rapidly from the blood (Blakley 1984). For cases in which a large dose of strychnine has been ingested, the stomach contents are the most suitable sample for toxicological analysis (Hatch and Funnell 1968, Blakley 1984, Hoogenboom and Ramell 1985). Up to 20 per cent of ingested strychnine is eliminated unmodified in the urine and up to 80 per cent is metabolised in the liver (Hatch 1988, Tanimoto and others 1990).…”

Section: Discussionmentioning

confidence: 99%

Atypical time course of clinical signs in a dog poisoned by strychnine

Meiser

2002

Veterinary Record

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The general health of a German shepherd dog had deteriorated slightly when it was found after being loose for one hour. After 10 hours of observation, the dog showed signs of pain for the first time and signs of poisoning, such as tenseness of muscles, slight opisthotonus, regurgitation, salivation, mydriasis, dyspnoea and cyanosis, were observed; it died 15 minutes after showing the first clinical signs but it had no seizures or tetanic spasms at any time. A postmortem examination did not reveal any pathological changes. A screening test for alkaloids was positive for strychnine (strychnidin-10-one). The presence of strychnine was confirmed and its concentration was determined by gas chromatography/mass spectrometry in urine (728.5 ng/ml) and in the stomach contents (44.6m microg/g). No strychnine was detected in the dog's serum, but traces of brucine (2,3-dimethoxystrychnidin-10-one), the dimethoxy derivative of strychnine, were detected. This case was compared with other strychnine poisonings recorded in the authors' laboratory over the previous six years, taking into account the species, type of samples, the clinical signs and their duration, the postmortem findings, and the concentrations of strychnine. This was the only case to show such an atypical time course of clinical signs.

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Section: Discussionmentioning

confidence: 99%

Atypical time course of clinical signs in a dog poisoned by strychnine

Meiser

2002

Veterinary Record

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“…An evaluation and modification of a microdiffusion method for the emergency determination of blood cyanide using Conway cells has been presented (37). The liquid chromatographic determination of strychnine in stomach contents has been accomplished by use of a reversed-phase column and an isocratic mobile phase (38). Strychnine levels were determined in postmortem blood, urine, bile, liver, kidney, stomach contents, small and large intestines, and brain by using a gas chromatograph equipped with a flame-ionization detector (39).…”

Section: Drugs and Poisonsmentioning

confidence: 99%

Forensic science

Brettell¹,

Saferstein²

1987

Anal. Chem.

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“…Solvents are traditionally used to extract the strychnine toxicant from strychnine-fortified grain baits as well as other biological matrices. The extracted strychnine is then quantified by high-performance liquid chromatography (HPLC) (Hoogenboom and Rammell, 1985; Hunter and Creekmur, 1984; Dennis, 1983;Alliot et al, 1982;), gas chromatography (Sharp, 1986;Bogusz et al, 1983;Miller et al, 1982), or spectrophotometry (Wapensky, 1969). Currently, organic solvents are coming under increasing scrutiny due to their cost and the environmental impact associated with their disposal (Snyder et al, 1993).…”

Section: Introductionmentioning

confidence: 99%