Liquid Chromatographic Determination of Phenolic Acids in Soil

Kelley, William Terry; Coffey, D. L.; Mueller, Thomas C.

doi:10.1093/jaoac/77.4.805

Cited by 30 publications

(23 citation statements)

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“…Furthermore, our extractants were acidified with TFA, which is more volatile than concentrated HCl (2 to 12 M). The use of HCl to acidify extractant solutions is common (2,8,10,13), but HCl may become concentrated as samples are taken to dryness, thus contributing to Phl degradation.…”

Section: Discussionmentioning

confidence: 99%

“…The solvent system described in this report is a simple linear gradient that avoids multigradient or multi-isocratic steps (8,16) and the complex solvent systems often used for the isolation of phenolic acids from soil (2,10). In addition, we performed reverse-phase chromatography with the column at room temperature rather than at 45ЊC (8), as the latter temperature may promote hydrolysis of Phl.…”

Section: Discussionmentioning

confidence: 99%

“…Comparison of acetone extraction with extraction procedures for plant phenolic acids from soil. Roots with adhering rhizosphere soil (15 g) from wheat grown in SSL were mixed with 30 ml of either 80% acetone, 250 mM EDTA at pH 7.0, 0.5 M NaOH, or 0.5 M sodium acetate (2,4,10). Each sample was amended with Phl (20 or 40 g) by method 3 and then shaken for 3 h at 200 rpm.…”

Section: Methodsmentioning

confidence: 99%

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Quantification of 2,4-Diacetylphloroglucinol Produced by Fluorescent Pseudomonas spp. In Vitro and in the Rhizosphere of Wheat

Bonsall¹,

Weller²,

Thomashow³

1997

Appl Environ Microbiol

134

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The broad-spectrum antibiotic 2,4-diacetylphloroglucinol (Phl) is a major determinant in the biological control of a wide range of plant diseases by fluorescent Pseudomonas spp. A protocol was developed to readily isolate and quantify Phl from broth and agar cultures and from the rhizosphere environment of plants. Extraction with ethyl acetate at an acidic pH was suitable for both in vitro and in situ sources of Phl. For soil samples, the addition of an initial extraction step with 80% acetone at an acidic pH was highly effective in eliminating polar organic soil components, such as humic and fulvic acids, which can interfere with Phl detection by high-performance liquid chromotography. The efficiency of Phl recovery from soil by a single extraction averaged 54.6%, and a second extraction added another 6.1%. These yields were substantially greater than those achieved by several standard protocols commonly used to extract polar phenolic compounds from soil. For the first time Phl was isolated from the rhizosphere environment in raw soil. Following application of Pseudomonas fluorescens Q2-87 and the Phl-overproducing strain Q2-87(pPHL5122) to the seeds of wheat, 2.1 and 2.4 g of Phl/g of root plus rhizosphere soil, respectively, were isolated from wheat grown in a Ritzville silt loam; 0.47 and 1.3 g of Phl/g of root plus rhizosphere soil, respectively, were isolated from wheat grown in a Shano silt loam. However, when the amount of Phl was calculated on the basis of cell density, Q2-87(pPHL5122) produced seven and six times more antibiotic than Q2-87 in Ritzville silt loam, and Shano silt loam, respectively.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Quantification of 2,4-Diacetylphloroglucinol Produced by Fluorescent Pseudomonas spp. In Vitro and in the Rhizosphere of Wheat

Bonsall¹,

Weller²,

Thomashow³

1997

Appl Environ Microbiol

134

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“…The analysis of the phenolics in the extracts was carried out using the method reported by Kelley, Coffey, and Mueller (). After extraction, the purified extracts (1 µL: 10:1 split) were analyzed by comparison with phenolic standards (Aldrich Chemical Co., Milwaukee, Wisconsin) by chromatography on a Hewlett‐Packard 6890 gas chromatograph (Hewlett‐Packard Corp., Palo Alto, California) equipped with a derivatized, non‐packed injection liner, a Rtx‐5MS (5% DIPHENTL‐95% Dimethyl polysiloxane) capillary column (30 m length, 0.25 mm column id., 0.25 µm film thickness), and detected with a flame ionization detector.…”

Section: Methodsmentioning

confidence: 99%

In vitro inhibitory effects of mistletoes (Loranthus begwensis L.) phenolic-rich extracts on α-amylase, α-glucosidase, and angiotensin converting enzyme activities

2018

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This study focused on the effect of phenolic-rich extracts of mistletoe from different host plants on key enzymes (a-amylase, a-glucosidase) linked to type-2 diabetes (T2D) and hypertension (angiotensin-I converting enzyme [ACE]), as well as Fe 21 and sodium nitroprusside (SNP)-induced thiobarbituric acid reactive species (TBARS) production in rat pancreas in vitro. The phenolic-rich extract was prepared using ethyl acetate and the effects on a-amylase, a-glucosidase, and ACE activities, and TBARS production was determined. The phenolic constituent of the extract was also determined. The results revealed that the extracts (CCT, KNT, AMT, BFT) inhibited a-amylase (EC 50 5 2.24, 2.59, 2.36, and 1.80 mg/mL, respectively), a-glucosidase (EC 50 5 1.94, 1.92, 1.96, and 8.59 mg/mL), and ACE (EC 50 5 0.87, 0.67, 0.75, and 1.06 mg/mL) activities, and Fe 21 and SNPinduced TBARS production. Therefore, inhibition of a-amylase, a-glucosidase, ACE, and oxidative stress by the phenolic-rich extracts could be among the mode of actions of mistletoe toward the management of T2D and hypertension. Practical applicationsDried mistletoe leaves are usually consumed as tea or decoction, and are often prescribed for hypertensive or diabetic patients while the wet leaves are used to cook soup. This study revealed the possible antidiabetic, antihypertensive, and antioxidant properties of mistletoe leaves from different host plants. K E Y W O R D Sdiabetes, enzyme, hypertension, mistletoe, phenolic

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“…Two stage extraction procedures followed for the removal of the phenolic compounds as described by Kelley, Coffey, and Mueller () and Provan, Scobbie, and Chesson (). After extraction, the sample was centrifuged (5,000 × g), rinsed with water, centrifuged again, and the supernatants combined and placed in a disposable glass test tube followed by heating at 90°C for 2 hr to release the conjugated phenolic compounds.…”

Section: Materials and Experimental Methodsmentioning

confidence: 99%

Effects of drying on cholinesterases and angiotensin-I converting enzyme inhibitory potential and phenolic constituents of African mistletoe (Loranthus bengwensis L) leaves from kolanut host tree

2018

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This study investigated the most appropriate drying method (sun drying, oven drying, and shade drying) for mistletoe leaves obtained from kolanut tree. The phenolic constituents were characterized using high performance liquid chromatography-diode array detector, while the inhibitory effect of the aqueous extracts of the leaves on cholinesterases and angiotensin-I converting enzyme (ACE) and antioxidant activities were determined in vitro. The extracts inhibited acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and ACE in dose-dependent manner. However, extract from sun-dried sample exhibited the highest AChE, BChE, and ACE inhibitory effect while extract from shade-dried sample had the least. Likewise, sun-dried sample exhibited the highest antioxidant properties as exemplified by Fe 21 -chelating, 1,1-diphenyl-2 picrylhdrazyl, OH, and nitric oxide radical scavenging abilities. This study also revealed the presence of 20 phenolic compounds with caffeic acid being the most predominant. Conclusively, kolanut host tree mistletoe leaves can be used as therapeutic agent in the management of Alzheimer's disease and hypertension. Practical applicationThe best drying method for mistletoe harvested from kolanut host tree was proposed in this study.Having identified the best drying method that retained phenolic constituents, antioxidant activities, anticholinesterase, and antihypertensive potential of kolanut host tree mistletoe leaves, this work maybe applicable in pharmaceutical industry for development of anticholinesterase and antihypertensive drugs with little or no side effect as well as in the food industry as complementary functional food/medicine in the management of Alzheimer's disease and hypertension.

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Liquid Chromatographic Determination of Phenolic Acids in Soil

Cited by 30 publications

References 0 publications

Quantification of 2,4-Diacetylphloroglucinol Produced by Fluorescent Pseudomonas spp. In Vitro and in the Rhizosphere of Wheat

Quantification of 2,4-Diacetylphloroglucinol Produced by Fluorescent Pseudomonas spp. In Vitro and in the Rhizosphere of Wheat

In vitro inhibitory effects of mistletoes (Loranthus begwensis L.) phenolic-rich extracts on α-amylase, α-glucosidase, and angiotensin converting enzyme activities

Effects of drying on cholinesterases and angiotensin-I converting enzyme inhibitory potential and phenolic constituents of African mistletoe (Loranthus bengwensis L) leaves from kolanut host tree

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