Liquid Chromatographic Determination of N-Methyl Carbamate Pesticide Residues at Low Parts-Per-Billion Levels in Eggs

Abstract

A reversed-phase liquid chromatographic (LC) method is presented for the analysis of N-methyl carbamate pesticide residues and piperonyl butoxide in eggs at levels as low as 2 gkg (ppb). The study was undertaken to provide data for dietary exposure estimates used in risk analysis. The method uses an acetonitrile extraction followed by liquidliquid partitioning and normal-phase aminopropyl solid-phase extraction column cleanup. Determination of residues is by reversed-phase LC with an inline postcolumn reaction followed by fluorescence detection. The average recoveries of 21 fortified (most at 2.0 and 20.0 ppb) N-methyl carbamate pesticide residues and the carbamate metabolite 1-naphthol from eggs ranged from 70 to 107%. Recoveries of the pesticide synergist piperonyl butoxide ranged from 63 to 106%. Single-comb White Leghorn hens were treated with the carbamate carbaryl, and the eggs subsequently produced were analyzed for carbaryl and 1-naphthol residues.

Chemical Residues and Contaminants in Foods of Animal Origin in Korea during the Past Decade

“…Table describes the outlines of residue analysis for veterinary drugs, pesticides, and dioxins in products of bovine, swine, and poultry. Residues of veterinary drugs were first analyzed using a bioassay for screening purposes and by HPLC and LC-MS/MS for confirmation and quantification. − Residues of pesticides were analyzed by HPLC, LC-MS, GC, and GC-MS. ,− Residues of dioxins were analyzed using isotope dilution high-resolution gas chromatography–mass spectrometry (HR-GC-MS). ,− Quality controls of the analyses were conducted, and the proficiency tests were conducted on a regular basis for samples obtained from FAPAS in the United Kingdom. The laboratories were accredited (ISO/IEC 17025) for the veterinary drugs, pesticides, and dioxin analyses.…”

Extraction Methodological Contributions Toward Ultra-Performance Liquid ChromatographyTime-of-Flight Mass Spectrometry: Quantification of Free GB from Various Food Matrices

“…By comparison, only limited literature exists regarding actual CWAs, such as sarin or its breakdown products, in food or beverages. [13][14][15] The pesticide literature often includes sample-preparation techniques that are commercially available and affordable, such as solid-phase extraction cartridges [16][17][18][19][20][21][22][23] or QuEChERS systems (Quick, Easy, Cheap, Effective, Rugged, and Safe). [24][25][26][27][28][29][30][31][32][33][34][35] However, the CWA literature seems to focus more on new techniques and specialized equipment that may not be readily accessible to every laboratory.…”

Trace Level Analysis of Sarin and VX in Food Using Normal Phase Silica Gel and Ultra-Performance Liquid Chromatography–Time-of-Flight Mass Spectrometry (UPLC–TOF–MS)

“…Numerous reviews exist on the low level detection of pesticides and provide the best examples for detecting organophosphorous compounds at low levels in food. − The actual chemical warfare agents themselves have only a limited number of papers dealing with sarin, VX, or even their breakdown products, in food or beverages. − While the pesticide literature often utilizes sample preparation techniques that are commercially available like solid phase extraction (SPE) cartridges − or QuEChERS systems (Quick, Easy, Cheap, Effective, Rugged, and Safe), − the CWA literature seems to have focused more on new techniques and specialized equipment that may not be as readily accessible to the everyday lab. − …”