Liquid chromatographic analysis of Hg(II) binding by thiol-rich peptides using both UV–vis and electrochemical detection

Characterization and classification of Spanish paprika (Capsicum annuum L.) by liquid chromatography coupled to electrochemical detection with screen-printed carbon-based nanomaterials electrodes

“…A number of analytical methods have been developed for the determination of polyphenols and the characterization of a great variety of plants and fruit-based products, being the use of liquid chromatography (LC) with ultra-violet (UV) detection or coupled to mass spectrometry (LC-MS) the most common one [10][11][12][13][14][15][16][17][18]. However, electrochemical detection (EC) appears as a very convenient alternative to the former detection modes taking advantage of its good sensitivity, low cost and the electroactive character of polyphenols [19,20].…”

New discrimination tools for harvest year and varieties of white wines based on hydrophilic interaction liquid chromatography with amperometric detection

“…However, UV and fluorescence detection methods require derivatisation treatments into UV absorbing or fluorescent species since these compounds present low UV-vis absorbance [6,19,20] whereas the detection by means of mass spectrometry is quite expensive. A more affordable alternative detection method that can avoid tedious derivatisation procedures is electrochemical detection, which takes advantage of the electroactive character of aminothiols, being the oxidation of thiol groups to disulfide the main electrochemical reaction involved [21][22][23][24][25][26][27][28].…”

Phytochelatin synthesis in response to Hg uptake in aquatic plants near a chlor-alkali factory

“…Among them the use of MS-HPLC is the most common, although detection by fluorescence or absorption spectroscopy is also considered (Serrano et al, 2015). In a previous work of our research group, a method to determine glutathione, PC n and their Hg complexes using amperometric detection in a glassy carbon electrode has been developed (Dago et al, 2009;. This method has been applied to study the effect that Hg and other metal ions have in the synthesis of PCs in Hordeum vulgare plants cultured in controlled conditions in the lab in the presence of these toxic substances (Dago et al, 2014a;2014b) or in Asparagus acutifolius plants growing naturally in the mining district of Almadén (Dago et al, 2014c).…”