Liquid Chromatographic Analysis of All-Rac-α-Tocopheryl Acetate, Tocopherols, and Retinyl Palmitate in SRM 1846

Chase, G William; Eitenmiller, Ronald R.; Long, Austin R

doi:10.1080/10826079708005833

Cited by 21 publications

(7 citation statements)

References 8 publications

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“…Classical approaches to the analysis of fat‐soluble vitamins in foodstuff require either a total lipid extraction followed by quantification by HPLC28 or an initial alkaline digestion step followed by an extraction with organic solvents of the vitamins from the unsaponifiable matter and quantification by HPLC 5–7,29. In our study, a saponification step was thought to be mandatory for the following reasons: (a) in products with high protein contents, a significant amount of vitamins can be bound to proteins, thus are not fully extracted with the total lipid extraction approach as with the saponification step;25 (b) a saponification step is recommended when analysing tocopherols by LC/MS, as phospholipids and phospholipid oxidation products may have [M+H] + ions or fragment ions similar to those of tocopherols with similar retention times 14.…”

Section: Resultsmentioning

confidence: 99%

Determination of α‐tocopherol in infant foods by liquid chromatography combined with atmospheric pressure chemical ionisation mass spectrometry

Kálmán

Mujahid

Mottier

et al. 2003

Rapid Comm Mass Spectrometry

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A novel, sensitive and specific method for the quantification of alpha-tocopherol in two infant foods (milk and cereals) using liquid chromatography on-line with positive atmospheric pressure chemical ionisation mass spectrometry detection (LC/APCI-MS) has been developed. The samples were first saponified in order to eliminate fats and to transform tocopherol esters into free tocopherol, followed up by a liquid-liquid extraction of the analyte in petroleum benzine/diisopropyl ether (75:25, v/v) prior to injection onto the LC system. For the quantification, deuterium-labelled tocopherol was used as internal standard and the samples were monitored in selected ion monitoring (SIM) mode. Calibration curves between 1-40 microg/mL of alpha-tocopherol showed a good linear correlation (r(2) = 0.99994), and the detection limit was determined to be 2.5 ng/mL. The within-day and between-day precision were determined for several dietetic infant formulae and certified reference samples, and found to be below 3.5%. The accuracy determined on a Nestlé reference sample (milk powder) was calculated to be 115.2 +/- 1.2%, which confirms the robustness of the proposed method. This study shows that single quadrupole LC/MS can be applied for the quantification of vitamins in food and the method offers better sensitivity and selectivity than traditional method such as LC-UV. This would simplify the preparation of the food samples and consequently enhance the vitamin analysis throughput in the food area.

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Section: Resultsmentioning

confidence: 99%

Determination of α‐tocopherol in infant foods by liquid chromatography combined with atmospheric pressure chemical ionisation mass spectrometry

Kálmán

Mujahid

Mottier

et al. 2003

Rapid Comm Mass Spectrometry

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“…The unknown in this exercise was a fortified milk powder, the control SRM 1849 Infant/Adult Nutritional Formula (45–48, 50, 51). The target value for retinol in the fortified milk powder was determined at NIST as part of an international interlaboratory comparison exercise.…”

Section: Resultsmentioning

confidence: 99%

Dietary Supplement Laboratory Quality Assurance Program:

Phillips¹,

Rimmer²,

Wood³

et al. 2012

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The National Institute of Standards and Technology (NIST) has established a Dietary Supplement Laboratory Quality Assurance Program (DSQAP) in collaboration with the National Institutes of Health Office of Dietary Supplements. Program participants measure concentrations of active and/or marker compounds as well as nutritional and toxic elements in food and dietary supplements distributed by NIST. Data are compiled at NIST, where they are analyzed for accuracy relative to reference values and concordance among the participants. Performance reports and certificates of completion are provided to participants, which can be used to demonstrate compliance with current Good Manufacturing Practices as promulgated by the U.S. Food and Drug Administration. The DSQAP has conducted five exercises to date, with total participation including more than 75 different laboratories and many more individual analysts.

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“…The starch content was determined by the method of Mestres and Mestres [ 17 ]. The vitamin E and ascorbic acid levels were determined by the (NP)-HPLC method [ 18 ] and the Association of Official Analytical Chemists (AOAC) method [ 19 ], respectively. The total carbohydrates and reducing sugar were determined by AOAC [ 19 ] procedures.…”

Section: Methodsmentioning

confidence: 99%

Innovation in Tigernut (Cyperus Esculentus L.) Milk Production: In Situ Hydrolysis of Starch

et al. 2020

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Tigernut tubers (Cyperus esculentus) are used for the production of vegetable milk, commonly known as “Horchata de chufa” in Spain. The presence of starch in the tuber limits the yield of the milk, since this carbohydrate gelatinizes during the pasteurization of the milk and leads to the considerable solidification of this drink. The present work aims to improve the yields and extraction practice of the milk by an in situ hydrolysis of starch, using exogenous amylases of industrial or vegetable origin. The obtained results show that sprouting improves the extraction yields of tigernut milk, which goes from 50% to about 70%. This improvement in milk yield corresponds to a hydrolysis of about 35% of the starch in the tuber. The use of exogenous amylases leads to starch hydrolysis rates of 45% and 70%, respectively, for amylolytic extracts from sprouted tigernut tubers and amylase, with the corollary of a natural increase in the sweetness of milk. This technical approach makes it possible to produce a naturally sweetened tigernut milk which easily lends itself to pasteurization without a significant increase in viscosity.

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Liquid Chromatographic Analysis of All-Rac-α-Tocopheryl Acetate, Tocopherols, and Retinyl Palmitate in SRM 1846

Cited by 21 publications

References 8 publications

Determination of α‐tocopherol in infant foods by liquid chromatography combined with atmospheric pressure chemical ionisation mass spectrometry

Determination of α‐tocopherol in infant foods by liquid chromatography combined with atmospheric pressure chemical ionisation mass spectrometry

Dietary Supplement Laboratory Quality Assurance Program:

Innovation in Tigernut (Cyperus Esculentus L.) Milk Production: In Situ Hydrolysis of Starch

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