Liquid Biopsy in Squamous Cell Carcinoma of the Esophagus and of the Head and Neck

Iacob, Răzvan; Mandea, Matei; Iacob, Speranţa; Pietroşanu, Cătălina; Paul, Doru; Hainăroşie, Răzvan; Gheorghe, Cristian

doi:10.3389/fmed.2022.827297

Cited by 10 publications

(8 citation statements)

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“…If PABPC1 expression is used as a biomarker to evaluate endoscopic patients, all PABPC1-HE patients should undergo further examination and treatment because the biomarker PPV was up to 100% (Monaghan et al , 2021). It is not noninvasive compared with liquid biopsy biomarkers in ESCC, such as exosome-related and serological miRNA markers (Iacob et al , 2022; Miyoshi et al , 2022). Thus, PABPC1 expression analysis should be more convenient since only immunohistochemistry is needed on endoscopic biopsy samples (Sukswai and Khoury, 2019).…”

Section: Discussionmentioning

confidence: 99%

Cytoplasmic poly(A)-binding protein 1 as a biomarker to assist early diagnosis and prognosis of esophageal squamous cell carcinoma in endoscopic biopsy fragments

Sun

et al. 2023

European Journal of Cancer Prevention

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Objective Esophageal squamous cell carcinoma (ESCC) has high mortality worldwide, but its early diagnosis and prognosis are very difficult. Cytoplasmic poly(A)-binding protein 1 (PABPC1) plays an important role in regulating most cellular processes, resulting in a close relationship to tumor genesis and malignant development. Therefore, this work aimed to evaluate the clinical value of PABPC1 as a biomarker for the early diagnosis and prognosis of ESCC in endoscopic patients. Methods A total of 185 patients with lesions found by endoscopy were involved in this study, including 116 finally diagnosed with ESCCs and 69 with nonmalignant lesions. Biopsy fragments and surgical specimens were collected to assess PABPC1 expression by immunohistochemistry, and the association between the expression and survival was analyzed and compared in both samples. Results The average ratio of positive tumor cells to total tumor cells in the biopsy fragments was lower than that in surgical specimens, leading to a cutoff value of only 10% for the former in ROC analysis (AOC = 0.808, P < 0.001). However, PABPC1 high expression (PABPC1-HE) in both biopsy fragments and surgical specimens was associated with poor survival. When PABPC1 expression was used as a biomarker to diagnose ESCC in biopsy fragments, sensitivity, specificity, positive predictive value, and negative predictive value reached 44.8, 100.0, 100.0, and 51.9%, respectively. Among the 116 ESCC patients, 32 received postoperative concurrent chemoradiotherapy. Postoperative treatment increased the overall survival (OS) but not disease-free survival in lymph node-positive patients (P = 0.007 and 0.957, respectively). Nevertheless, PABPC1-HE predicted shorter OS regardless of the postoperative treatment in both endoscopic biopsy samples and surgical specimens. Conclusion PABPC1 expression can be used as a biomarker to detect ESCC from endoscopic lesions. At the same time, PABPC1-HE is a predictor of poor survival regardless of postoperative chemoradiotherapy in endoscopic biopsy samples of ESCC.

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Section: Discussionmentioning

confidence: 99%

Cytoplasmic poly(A)-binding protein 1 as a biomarker to assist early diagnosis and prognosis of esophageal squamous cell carcinoma in endoscopic biopsy fragments

Sun

et al. 2023

European Journal of Cancer Prevention

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“…16 Further, the adaptability of TOFS technology facilitates its application in identifying patients likely to benefit from certain target immunotherapies, such as the detection of PD-1 and PD-L2 biomarkers to predict responsiveness to checkpoint inhibitor therapy. 17,18 In addition to the utility of TOFS in biomarker detection in the clinic, its application in liquid biopsy has the potential to bolster research in salivary biomarkers of HNSCC.…”

Section: Discussionmentioning

confidence: 99%

The Application of Label-free Detection Using a Tapered Optical Fiber System for Head and Neck Cancer and Infectious Biomolecules

Collet,

Deng,

Ranathunga

et al. 2023

Preprint

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IntroductionHead and neck squamous cell carcinoma (HNSCC) is associated with high morbidity and mortality due to late detection. Tapered optical fiber sensors (TOFS) are biosensors with the potential application as a point-of-care device for detection of HNSCC biomarkers. TOFS uses optical fibers as transduction elements and antigen-antibody binding for the detection of target biomolecules. The present TOFS system was designed to achieve high specificity, sensitivity, and repeatability. To explore its application in HNSCC, we targeted the proinflammatory cytokine IL-8, known for its role in promoting tumorigenicity, metastasis, and angiogenesis in HNSCC. To validate our proof-of-concept experiment, a viral surrogate of SARS-CoV-2, Human Coronavirus OC43 (HCoV-OC43), was also tested.MethodsOur TOFS system contains four main parts: the tapered optical fiber, reservoir (cell), laser light source, and photodetector. The fiber is bitapered with a narrow waist region that anchors antibodies to detect target biomolecules. Light is transduced by the laser to the fiber where it travels through the down-taper region to the tapered waist followed by the up-tapered region and ultimately transmitted to the photodetector. The tapered waist facilitates the transformation of a single mode light to multiple modes, which reverts to a single mode of light as it continues through the up-taper region. Biomolecules introduced to the waist region alter the effective refractive index, which is reflected in the phase change of the wavelength. This is detected by the photodetector and quantified using Fouier analysis. Our tapered fibers were designed with antigen-antibody complexes targeting IL-8 and HCoV-OC43 to validate our TOFS design across two different antigens.ResultsTOFS with tethered mouse anti-human IgG (28nm) bound to dissolved IL-8 (14 nm) produces a measurable phase shift. As little as 10pg/ml or 7.1X105IL-8 molecules/μl was detected by the prototype device and the phase change represented in real-time the binding dynamic of IL-8 to the tethered IgG. To validate our proof-of-concept experiment, HCoV-OC43 dissolved in saliva was detected with a sensitivity of 50 viruses/mL.ConclusionsTOFS device is a highly sensitive system capable of detecting proteins, viruses, and other biomolecules. Selectivity of the system is guaranteed by specific antigen-antibody binding, supported by the detection of IL-8 and HCoV-OC43 at the femtomolar level. The long-term goal for TOFS is its application as a point-of-care device used for detection, monitoring, and surveillance of HNSCC as well as detection of other pathogens in the clinical setting.

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“…The cfDNA template is found in specific body fluids at very low concentrations, which may lead to the low sensitivity of detection. A growing body of evidence suggests that liquid biopsy has recently emerged as a new noninvasive technique of detecting blood circulating biomarkers for OSCC [116], e.g., detection of circulating tumor cells (CTCs) and circulating tumor DNA (ctDNA) [117,118]. Authors have reported that a liquid biopsy is a novel tool for diagnosis, prognostic stratification, and personalized therapy, while liquid biopsy biomarkers for OSCC could be useful in early detection and may provide prognosis information [119].…”

Section: Znf582mentioning

confidence: 99%

Exploring Potential Biomarkers in Oesophageal Cancer: A Comprehensive Analysis

Romanowicz,

Lukaszewicz-Zajac,

Mroczko

2024

IJMS

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Oesophageal cancer (OC) is the sixth leading cause of cancer-related death worldwide. OC is highly aggressive, primarily due to its late stage of diagnosis and poor prognosis for patients’ survival. Therefore, the establishment of new biomarkers that will be measured with non-invasive techniques at low cost is a critical issue in improving the diagnosis of OC. In this review, we summarize several original studies concerning the potential significance of selected chemokines and their receptors, including inflammatory proteins such as interleukin-6 (IL-6) and C-reactive protein (CRP), hematopoietic growth factors (HGFs), claudins (CLDNs), matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs), adamalysines (ADAMs), as well as DNA- and RNA-based biomarkers, in OC. The presented results indicate the significant correlation between the CXCL12, CXCR4, CXCL8/CXCR2, M-CSF, MMP-2, MMP-9 ADAM17, ADAMTS-6, and CLDN7 levels and tumor stage, as well as the clinicopathological parameters of OC, such as the presence of lymph node and/or distant metastases. CXCL12, CXCL8/CXCR2, IL-6, TIMP-2, ADAM9, and ADAMTS-6 were prognostic factors for the overall survival of OC patients. Furthermore, IL-6, CXCR4, CXCL8, and MMP-9 indicate higher diagnostic utility based on the area under the ROC curve (AUC) than well-established OC tumor markers, whereas CLDN18.2 can be used in novel targeted therapies for OC patients.

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Liquid Biopsy in Squamous Cell Carcinoma of the Esophagus and of the Head and Neck

Cited by 10 publications

References 125 publications

Cytoplasmic poly(A)-binding protein 1 as a biomarker to assist early diagnosis and prognosis of esophageal squamous cell carcinoma in endoscopic biopsy fragments

Cytoplasmic poly(A)-binding protein 1 as a biomarker to assist early diagnosis and prognosis of esophageal squamous cell carcinoma in endoscopic biopsy fragments

The Application of Label-free Detection Using a Tapered Optical Fiber System for Head and Neck Cancer and Infectious Biomolecules

Exploring Potential Biomarkers in Oesophageal Cancer: A Comprehensive Analysis

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