Liquid biopsy in colorectal cancer: Onward and upward

Torresan, Sara; de Scordilli, Marco; Bortolot, Martina; Di Nardo, Paola; Foltran, Luisa; Fumagalli, Arianna; Guardascione, Michela; Ongaro, Elena; Puglisi, Fabio

doi:10.1016/j.critrevonc.2023.104242

Cited by 4 publications

(2 citation statements)

References 196 publications

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“…The application of ctDNA technology in oncology opens new avenues for understanding and managing various cancers. Nowadays, there exists an extensive body of literature focusing on the applicability of ctDNA in various malignancies, particularly in lung cancer 36 , 37 , colorectal cancer 38 , 39 , gasrtic cancer 40 , 41 , pancreatic cancer 42 , 43 , liver cancer 44 , 45 , and breast cancer 46 , 47 . However, there is a discernible gap in research pertaining to esophageal cancer within this domain.…”

Section: Discussionmentioning

confidence: 99%

Association between plasma circulating tumor DNA and the prognosis of esophageal cancer patients: a meta-analysis

Zhang,

Jin,

Peng

et al. 2024

International Journal of Surgery

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Background: The application of liquid biopsy analysis utilizing circulating tumor DNA (ctDNA) has gained prominence as a biomarker in specific cancer types. Nevertheless, the correlation between ctDNA and the prognostic outcomes of patients with esophageal cancer (EC) remains a subject of controversy. This meta-analysis aims to assess the correlation between ctDNA and the prognosis of EC patients. Methods: We systematically explored Embase, PubMed, and the Cochrane Database to identify studies reporting on the prognostic value of ctDNA in EC patients before November 2023. The primary outcome involved the determine of associations between ctDNA with overall survival (OS), disease-free survival (DFS)/recurrence-free survival (RFS), as well asprogression-free survival (PFS) among EC patients. Secondary outcomes encompassed a detailed subgroup analysis in the setting of EC, including parameters such as detection time, histological subtypes, treatment modalities, regions, anatomic locations, and detection methods. Publication bias was assessed utilizing Begg’s test, Egger’s test, and funnel plots. A sensitivity analysis was conducted by systematically excluding individual studies to evaluate the stability of the results. Results: A total of 1203 studies were initially screened, from which 13 studies underwent further analysis, encompassing 604 patients diagnosed with EC. The comprehensive pooled analysis indicated a significant association between the detection of ctDNA and poor OS (HR: 3.65; 95% CI: 1.97–6.75, P<0.001), DFS/RFS (HR: 6.08; 95% CI: 1.21–30.50, P<0.001), and PFS (HR: 2.84; 95% CI: 1.94–4.16, P<0.001). Subgroup analysis showed that ctDNA remained a consistent negative predictor of OS when stratified by different detection time, histological subtypes, regions, anatomic locations, and detection methods. Furthermore, subgroup analysis stratified by regions and study types demonstrated an association between ctDNA detection and poor PFS in EC patients. Conclusion: Our results indicate plasma ctDNA may serve as robust prognostic markers for OS, DFS/RFS, and PFS among EC patients. This finding suggests that plasma ctDNA could offer a highly effective approach for risk stratification and personalized medicine.

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Section: Discussionmentioning

confidence: 99%

Association between plasma circulating tumor DNA and the prognosis of esophageal cancer patients: a meta-analysis

Zhang,

Jin,

Peng

et al. 2024

International Journal of Surgery

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“…Cancer is fundamentally a disease caused by genetic mutations that disrupt the delicate homeostasis of cellular functions (1). For liquid biopsy of cancers, circulating cell-free DNA (cfDNA) has been explored as a potential biomarker for cancer screening and monitoring (2)(3)(4). Typically, biomarker isolation is one of three important steps in liquid biopsy workflow (5).…”

Section: Introductionmentioning

confidence: 99%

Ampholytic ion-exchange magnetic beads: a promising tool for selecting short fragments in circulating cell-free DNA analysis

He,

Wang,

Zhou

et al. 2024

Front. Oncol.

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ObjectiveFor liquid biopsy of cancer, the extraction of circulating cell-free DNA (cfDNA) from plasma is required. We evaluated the efficacy of use of magnetic submicron particles coated with abundant small zwitterions (MSP-ZEWBs) for extracting short fragments of cfDNA.MethodsWe developed and optimized an MSP-ZEWB-based cfDNA extraction method using ampholytic ion-exchange materials and compared its results with those using a control kit. We measured the cfDNA concentration by quantitative polymerase-chain-reaction and using the Qubit method and analyzed cfDNA fragmentation patterns using a bioanalyzer.ResultsThe fragment size of cfDNA isolated from glycine hydrochloric acid at a pH of 2.2 exhibited a better alignment with the DNA marker. The highest DNA intensity was observed at the final concentration of 0.8% polyethylene glycol 8000. The intensity of cfDNA decreased significantly when isolated from plasma with DNA marker using MSP-ZEWBs with an adsorption buffer containing guanidine hydrochloride or isothiocyanoguanidine. All fragments were successfully extracted using MSP-ZEWBs from both plasma and phosphate-buffered saline. Notably, the intensity of short cfDNA fragments isolated using MSP-ZEWBs remained consistent for recovery of long DNA fragments. indicating a potential selective of small fragments.ConclusionThe extraction of plasma cfDNA with MSP-ZEWBs requires no protein denaturation, shows resistance to cells remaining in plasma, and demonstrates higher overall efficiency and better reproducibility than other extraction methods. Use of MSP-ZEWBs may greatly enhance liquid biopsy of cancers through the analysis of plasma cfDNA in clinical practice.

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Textural heterogeneity of liver lesions in CT imaging - comparison of colorectal and pancreatic metastases

Pietsch,

Haag,

Ayx

et al. 2024

Abdom Radiol

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Purpose Tumoral heterogeneity poses a challenge for personalized cancer treatments. Especially in metastasized cancer, it remains a major limitation for successful targeted therapy, often leading to drug resistance due to tumoral escape mechanisms. This work explores a non-invasive radiomics-based approach to capture textural heterogeneity in liver lesions and compare it between colorectal cancer (CRC) and pancreatic cancer (PDAC). Materials and methods In this retrospective single-center study 73 subjects (42 CRC, 31 PDAC) with 1291 liver metastases (430 CRC, 861 PDAC) were segmented fully automated on contrast-enhanced CT images by a UNet for medical images. Radiomics features were extracted using the Python package Pyradiomics. The mean coefficient of variation (CV) was calculated patient-wise for each feature to quantify the heterogeneity. An unpaired t-test identified features with significant differences in feature variability between CRC and PDAC metastases. Results In both colorectal and pancreatic liver metastases, interlesional heterogeneity in imaging can be observed using quantitative imaging features. 75 second-order features were extracted to compare the varying textural characteristics. In total, 18 radiomics features showed a significant difference (p < 0.05) in their expression between the two malignancies. Out of these, 16 features showed higher levels of variability within the cohort of pancreatic metastases, which, as illustrated in a radar plot, suggests greater textural heterogeneity for this entity. Conclusions Radiomics has the potential to identify the interlesional heterogeneity of CT texture among individual liver metastases. In this proof-of-concept study for the quantification and comparison of imaging-related heterogeneity in liver metastases a variation in the extent of heterogeneity levels in CRC and PDAC liver metastases was shown.

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Liquid biopsy in colorectal cancer: Onward and upward

Cited by 4 publications

References 196 publications

Association between plasma circulating tumor DNA and the prognosis of esophageal cancer patients: a meta-analysis

Association between plasma circulating tumor DNA and the prognosis of esophageal cancer patients: a meta-analysis

Ampholytic ion-exchange magnetic beads: a promising tool for selecting short fragments in circulating cell-free DNA analysis

Textural heterogeneity of liver lesions in CT imaging - comparison of colorectal and pancreatic metastases

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