Liquid-Based Cytology Preparation Can Improve Cytological Assessment of Endobronchial Ultrasound-Guided Transbronchial Needle Aspiration

Abstract

Objective: This study investigated whether liquid-based cytology (LBC) can improve diagnostic values of cytological assessment of endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA). Study Design: A cohort of 600 cases in West China Hospital was prospectively studied from June 2012 to September 2013. EBUS-TBNA was carried out in outpatients under local anesthesia and moderate sedation. The procedure was performed with an echobronchoscope (BF-UC160F-OL8, Olympus, Tokyo, Japan). Histological cores were stained with hematoxylin and eosin for further study. Additional immunohistological analysis was performed for establishing a reliable diagnosis when necessary. Aspirates were smeared on glass slides and separate aspirates were processed by the monolayer SurePath method. Results: In total, 480 malignant tumors and 120 benign lesions were confirmed by histological examination. The sensitivity of SurePath liquid-based preparations and conventional smears was 82.1 and 56%, and the specificity was 87.5 and 82.5%, respectively. The combined specificity was 100%. Positive predictive values of the two groups were 96.3 and 92.8%, whereas negative predictive values were 54.9 and 31.9%, respectively. The difference between the two groups was significant (p < 0.001). Conclusions: LBC preparation can improve cytological assessment of EBUS-TBNA. Histological study is necessary in cases in which the cytological diagnosis is obscure.

Evaluation of DNA and RNA quality from archival formalin‐fixed paraffin‐embedded tissue for next‐generation sequencing – Retrospective study in Japanese single institution

“…In general, long-term stored FFPE tissue samples displayed significant DNA fragmentation, and a library of amplicons of approximately 200 bp should be synthesized as a template for PCR. 22,[31][32][33][34][35] In this study, NGS analysis was performed using almost 100% tumor tissue. However, when a non-tumor component is included in the specimen for analysis, it introduces an unknown of whether the result reflects a tumor-derived gene or not.…”

“…However, comprehensive analysis by NGS enables the sequencing results of many amplicons with a short base pair length at the same time, demonstrating that genetic analysis can also be performed on DNA extracted from FFPE tissues, and making it easier to introduce genetic analysis into pathological diagnosis. [21][22][23][24][25] Despite FFPE use, there is a debate of whether FFPE applicable to genetic analysis can maintain satisfactory quality by avoiding damage to nucleic acids, due to formalin fixation and degradation of nucleic acids based on long-term storage in a paraffin block. Visual fragmentation confirmation by electrophoresis and Ct value by quantitative PCR are used as quality parameters for nucleic acids.…”

Genotyping and cytomorphological subtyping of lung adenocarcinoma based on liquid‐based cytology

“…[20][21][22][23][24] LBC material can thus be used for molecular testing and routine cytological diagnoses, as well as for immunocytochemical staining and in situ hybridization. A few studies have used LBC material for molecular testing in lung cancer, 25,26 but further studies are needed to validate its feasibility for use in clinical practice. In the current study, we detected several major gene aberrations (EGFR, KRAS, ALK, and ROS1) using LBC material from patients with lung ADC.…”

Liquid-based cytopathology test as a novel method to identify Aspergillus in patients with pulmonary aspergillosis

“…Identification of Aspergillus from stained cytological slides plays an important role in the diagnosis and management of pulmonary aspergillosis, but conventional slide preparation has several disadvantages that make Aspergillus identification in respiratory samples challenging 19 , 20 . In this study, we compared the ability of LCT and conventional cytopathology to allow Aspergillus identification in sputum and bronchial brushing samples following Papanicolaou’s or Special staining.…”