Abstract:Essential oils from Lavandula genus and the obtained hybrids are widely used for different purposes such as perfume production in the cosmetic field and for its biological properties. This is the first study on the liquid and vapour phase of Lavandula × intermedia “Grosso” essential oil grown in the Lazio Region, Italy, investigated using headspace coupled to gas chromatography and mass spectrometry (HS-GC/MS). The results showed the most abundant components were linalool and linalyl acetate, followed by 1,8-c… Show more
“…This technique is ideal for sampling volatiles present in solid and liquid matrices [ 19 , 20 ]. With the intent to optimize the headspace sampling procedure for the determination of volatile organic compounds (VOCs), some parameters, such as equilibration time, temperature and head space analysis duration, were adjusted [ 21 ]. The sampling procedure was thus carried out: sliced fruits and homogenates were collected into a 20 mL vial and tightly sealed immediately with crimp aluminum caps and 20-mm white rubber septa (Supelco, PA, USA) using a vial crimper.…”
Strawberries belonging to cultivar Clery (Fragaria x ananassa (Duchesne ex Weston)), cultivated in central Italy were subjected to a multi-methodological experimental study. Fresh and defrosted strawberries were exposed to different processing methods, such as homogenization, thermal and microwave treatments. The homogenate samples were submitted to CIEL*a*b* color analysis and Head-Space GC/MS analysis to determine the impact of these procedures on phytochemical composition. Furthermore, the corresponding strawberry hydroalcoholic extracts were further analyzed by HPLC-DAD for secondary metabolites quantification and by means of spectrophotometric in vitro assays to evaluate their total phenolic and total flavonoid contents and antioxidant activity. These chemical investigations confirmed the richness in bioactive metabolites supporting the extraordinary healthy potential of this fruit as a food ingredient, as well as functional food, highlighting the strong influence of the processing steps which could negatively impact on the polyphenol composition. Despite a more brilliant red color and aroma preservation, non-pasteurized samples were characterized by a lower content of polyphenols and antioxidant activity with respect to pasteurized samples, as also suggested by the PCA analysis of the collected data.
“…This technique is ideal for sampling volatiles present in solid and liquid matrices [ 19 , 20 ]. With the intent to optimize the headspace sampling procedure for the determination of volatile organic compounds (VOCs), some parameters, such as equilibration time, temperature and head space analysis duration, were adjusted [ 21 ]. The sampling procedure was thus carried out: sliced fruits and homogenates were collected into a 20 mL vial and tightly sealed immediately with crimp aluminum caps and 20-mm white rubber septa (Supelco, PA, USA) using a vial crimper.…”
Strawberries belonging to cultivar Clery (Fragaria x ananassa (Duchesne ex Weston)), cultivated in central Italy were subjected to a multi-methodological experimental study. Fresh and defrosted strawberries were exposed to different processing methods, such as homogenization, thermal and microwave treatments. The homogenate samples were submitted to CIEL*a*b* color analysis and Head-Space GC/MS analysis to determine the impact of these procedures on phytochemical composition. Furthermore, the corresponding strawberry hydroalcoholic extracts were further analyzed by HPLC-DAD for secondary metabolites quantification and by means of spectrophotometric in vitro assays to evaluate their total phenolic and total flavonoid contents and antioxidant activity. These chemical investigations confirmed the richness in bioactive metabolites supporting the extraordinary healthy potential of this fruit as a food ingredient, as well as functional food, highlighting the strong influence of the processing steps which could negatively impact on the polyphenol composition. Despite a more brilliant red color and aroma preservation, non-pasteurized samples were characterized by a lower content of polyphenols and antioxidant activity with respect to pasteurized samples, as also suggested by the PCA analysis of the collected data.
“…Industrial hemp was used to obtain essential oil rich in terpenic compounds and this oil was tested against pathogenic and spoilage microorganisms to evaluate the antimicrobial activity [10]. Lavandin essential oil (liquid and vapour phase) showed an antimicrobial activity against gram-negative (E. coli, Acinetobacter bohemicus, and Pseudomonas fluorescens) and gram-positive (B. cereus and Kocuria marina) bacteria [11].…”
Schinus molle (L.) is a dioecious plant of the Anacardiaceae family, originating in South America and currently widespread in many regions throughout the world. In this work leaf extracts and derived low-pressure column chromatography (LPCC) fractions of S. molle L. male and female plants were investigated for the antimicrobial activity. Leaf extracts were tested on microbes Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Enterococcus faecalis, Candida albicans and Bacillus subtilis. Furthermore, the extracts showing antimicrobial activity were fractionated by LPCC and the obtained fractions tested on the same microorganism strains. Positive fractions were investigated by gas-chromatography/mass spectrometry (GC-MS) and were seen to be rich in sesquiterpenes, sesquiterpenoids and other terpens. The obtained effects highlighted the antimicrobial properties of S. molle (L.) leaf compounds and revealed their importance as a source of bioactive molecules of potential pharmaceutical interest. To our knowledge, this is the first paper reporting investigations on the chemical composition of the extracts and derived positive fractions from Schinus molle (L.) plants grown in central Italy
“…As we already reported [19], LEO mainly consists of four compounds belonging to the monoterpene family: linalool (41.6%) linalyl acetate (23.0%), 1,8-cineole (5.2%), and terpinen-4-ol (4.8%). In this paper we aimed to extend knowledge on LEO's anticancer properties by carrying out investigations on HL60 human leukemia cells.…”
Section: Discussionmentioning
confidence: 77%
“…In our previous work [19], we investigated the chemical composition of LEO by GC/MS and highlighted that terpinen-4-ol, linalyl acetate, linalool, and 1,8-cineole are main LEO compounds. In this work, we investigated which of these compounds were capable of inducing an apoptotic effect.…”
Section: Mtt and Flow Cytometry Analysis Of Leo Main Compoundsmentioning
confidence: 99%
“…As reported in our previous work [19], Lavandin Essential Oil (LEO) mainly consists of four compounds belonging to the monoterpene family: terpinen-4-ol, linalyl acetate, linalool, and 1,8-cineole, which have been previously examined for their antitumor activity [20][21][22][23].…”
Recent scientific investigations have reported a number of essential oils to interfere with intracellular signalling pathways and to induce apoptosis in different cancer cell types. In this paper, Lavandin Essential Oil (LEO), a natural sterile hybrid obtained by cross-breeding L. angustifolia × L. latifolia, was tested on human leukaemia cells (HL60). Based on the MTT results, the reduced cell viability of HL60 cells was further investigated to determine whether cell death was related to the apoptotic process. HL60 cells treated for 24 h with LEO were processed by flow cytometry, and the presence of Annexin V was measured. The activation of caspases-3 was evaluated by western blot and immunofluorescence techniques. Treated cells were also examined by scanning and transmission electron microscopy to establish the possible occurrence of morphological alterations during the apoptotic process. LEO main compounds, such as linalool, linalyl acetate, 1,8-cineole, and terpinen-4-ol, were also investigated by MTT and flow cytometry analysis. The set of obtained results showed that LEO treatments induced apoptosis in a dose-dependent, but not time-dependent, manner on HL60 cells, while among LEO main compounds, both terpinen-4-ol and linalyl acetate were able to induce apoptosis.
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