Liquid and solid selective differential media for the detection and enumeration of L. monocytogenes and other Listeria spp.

Netten, P. van; Perales, Ildefonso; Moosdijk, A. van de; Curtis, Gordon D W; Mossel, D. À. A.

doi:10.1016/0168-1605(89)90001-9

Cited by 186 publications

(75 citation statements)

References 22 publications

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“…On PALCAM agar, Listeria colonies appear grey-green, are approximately 2 mm in diameter, and have black, sunken centers. They also have a black halo against a cherry red background (165). On Oxford agar, L. monocytogenes colonies appear black, are 1 mm (24 h) to 3 mm (48 h) in diameter, and are surrounded by black halos (27) (87,132).…”

Section: Microbiology Of L Monocytogenesmentioning

confidence: 99%

“…Growth on these media is examined under oblique 450 illumination to detect the typical light blue colonies formed by Listeria species. The PALCAM medium developed by van-Netten et al (165) and the Oxford agar developed by Curtis et al (27) attempt to overcome this disadvantage by incorporating differential agents in the agar medium. On PALCAM agar, Listeria colonies appear grey-green, are approximately 2 mm in diameter, and have black, sunken centers.…”

Section: Microbiology Of L Monocytogenesmentioning

confidence: 99%

“…Among the most frequently used selective chemical enrichment media are (i) Food and Drug Administration (FDA) broth, an enrichment broth prepared from tryptic soy broth, acriflavine, and nalidixic acid (95,97); (ii) U.S. Department of Agriculture-Food Safety and Inspection Service (USDA-FSIS) broth, containing esculin, acriflavin, and nalidixic acid; developed by Dominguez Rodriguez et al (31), modified by Donnelly and Baigent (32), and used by the USDA-FSIS for the isolation of L. monocytogenes from meats and poultry products (109); (iii) nutrient broth containing 3.75% potassium thiocyanate and nalidixic acid, developed by Watkins and Sleath (167) and used by Hayes et al. (59) to isolate L. monocytogenes from epidemic-associated raw milk; and (iv) L-PALCAM broth, a medium developed in The Netherlands (165) and used widely in Europe. Foods are enriched in these media for 24 to 48 h and plated on Listeria-selective agars.…”

Section: Microbiology Of L Monocytogenesmentioning

confidence: 99%

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Epidemiology of human listeriosis

1991

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During the 1980s, investigation of several large epidemics of listeriosis confirmed that transmission of L. monocytogenes in food causes human disease. Progress in laboratory detection and subtyping of the organism has enhanced our ability to compare human and environmental isolates of L. monocytogenes. Transmission by foodborne organisms is now recognized as causing both epidemic and sporadic listeriosis. Continued study of dietary risk factors associated with listeriosis is needed in order to develop dietary recommendations for the expanding population at increased risk of disease. Current research application of new molecular methods to the study of L. monocytogenes may improve the ability to diagnose pregnancy-associated disease and permit the rapid detection and control of L. monocytogenes in the food supply.

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Section: Microbiology Of L Monocytogenesmentioning

confidence: 99%

Section: Microbiology Of L Monocytogenesmentioning

confidence: 99%

Section: Microbiology Of L Monocytogenesmentioning

confidence: 99%

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Epidemiology of human listeriosis

1991

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“…At indicated time points, organs were harvested and homogenized in PBS. Serial dilutions of the resulting organ suspensions were plated on Palcam-agar plates (24) and CFUs were counted 24 -48 h after culture at 37°C.…”

Section: Bacterial Titersmentioning

confidence: 99%

Immunoproteasomes Are Essential for Clearance ofListeria monocytogenesin Nonlymphoid Tissues but Not for Induction of Bacteria-Specific CD8+ T Cells

Strehl

Joeris

Rieger

et al. 2006

The Journal of Immunology

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Microbial infections induce the replacement of constitutive proteasomes by immunoproteasomes (I-proteasomes). I-proteasomes support efficient generation of MHC class I epitopes and influence immunodominance hierarchies of CD8+ T cells. Recently, the function of I-proteasomes in antimicrobial responses was challenged by showing that the lack of I-proteasomes has no effect on induction and function of lymphocytic choriomeningitis virus-specific CD8+ T cells. Here, we show that infection with Listeria monocytogenes rapidly induces I-proteasomes in nonlymphoid tissues, which leads to enhanced generation of protection relevant CD8+ T cell epitopes. I-proteasome-deficient mice (β5i−/− mice) exhibited normal frequencies of L. monocytogenes-specific CD8+ T cells. However, clearance of L. monocytogenes in liver but not spleen was significantly impaired in I-proteasome-deficient mice. In summary, our studies demonstrate that induction of I-proteasomes is required for CD8+ T cell-mediated elimination of L. monocytogenes from nonlymphoid but not lymphoid tissues.

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“…Among the possible causes for contamination were the continuous presence of water on floors adjacent to packing equipment, failure to properly clean and sanitize drains and food contact surfaces, and unregulated traffic patterns that may have established routes for L. monocytogenes contamination to occur (55). Although there have been no reports of listeriosis directly attributed to consumption of fresh mushrooms, retail surveys indicate that L. monocytogenes contamination can occur (32,46,56). Several recalls have occurred after detectable levels of L. monocytogenes were found on whole or sliced mushrooms.…”

mentioning

confidence: 99%

Incidence of Listeria monocytogenes and Listeria spp. in a Small-Scale Mushroom Production Facility

Viswanath

Murugesan

Knabel

et al. 2013

Journal of Food Protection

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Listeria monocytogenes is a foodborne pathogen of significant concern to the agricultural and food processing industry because of its ability to grow and persist in cool and moist environments and its association with listeriosis, a disease with a very high mortality rate. Although there have been no listeriosis outbreaks attributed to fresh mushrooms in the United States, retail surveys and recalls are evidence that L. monocytogenes contamination of mushrooms (Agaricus bisporus) can occur. The objective of this study was to determine the prevalence of Listeria spp., including L. monocytogenes, in a small-scale mushroom production facility on the campus of the Pennsylvania State University in the United States. Of 184 samples taken from five production zones within the facility, 29 (15.8%) samples were positive for Listeria spp. Among the Listeria spp. isolates, L. innocua was most prevalent (10.3%) followed by L. welshimeri (3.3%), L. monocytogenes (1.6%), and L. grayi (0.5%). L. monocytogenes was recovered only from the phase I raw material composting area. Isolates of L. monocytogenes were confirmed and serotyped by multiplex PCR. The epidemiological relatedness of the three L. monocytogenes isolates to those serotypes or lineages frequently encountered in listeriosis infections was determined by multi-virulence-locus sequence typing using six virulence genes, namely, prfA, inlB, inlC, dal, clpP, and lisR. The phylogenetic positions of the three isolates in the dendrogram prepared with data from other isolates of L. monocytogenes showed that all isolates were grouped with serotype 4a, lineage IIIA. To date, this serotype has rarely been reported in foodborne disease outbreaks.

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Liquid and solid selective differential media for the detection and enumeration of L. monocytogenes and other Listeria spp.

Cited by 186 publications

References 22 publications

Epidemiology of human listeriosis

Epidemiology of human listeriosis

Immunoproteasomes Are Essential for Clearance ofListeria monocytogenesin Nonlymphoid Tissues but Not for Induction of Bacteria-Specific CD8+ T Cells

Incidence of Listeria monocytogenes and Listeria spp. in a Small-Scale Mushroom Production Facility

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