Liquefaction of sugarcane bagasse for enzyme production

Cunha, F. M.; Kreke, Thomas; Badino, Alberto Colli; Farinas, Cristiane S.; Ximenes, Eduardo; Ladisch, Michael R.

doi:10.1016/j.biortech.2014.09.025

Cited by 34 publications

(11 citation statements)

References 17 publications

(20 reference statements)

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“…This band was more intense for WB and was poorly defined for SB, as expected due to the low lignin and hemicellulose contents of the latter biomass [39,41]. The images are actual size a Rodriguez-Zuniga et al [34]; b Cunha et al [31]; c Ratio between carbon and nitrogen in each material Bands near 1643, 1605, and 1501 cm -1 are frequently assigned to the lignin structure (C-Ph vibration, and C=O and C=C aromatic ring skeletal vibrations of lignin) [39,40,42]. The band at 1640 cm -1 in the SCB p spectrum was a possible indication of the conversion of lignin methoxyl groups to phenolic groups during the steam explosion pretreatment.…”

Section: Resultsmentioning

confidence: 98%

“…The chemical composition of the steam explosion-pretreated sugarcane bagasse was determined previously [31], following the NREL procedure described by Sluiter et al [32,33]. The chemical compositions of the wheat bran, soybean bran, and untreated sugarcane bagasse samples were determined previously in our laboratory, as described by Rodriguez-Zuniga et al [34].…”

Section: Agro-industrial Residues Characterizationmentioning

confidence: 99%

See 1 more Smart Citation

On-Site Production of Enzymatic Cocktails Using a Non-conventional Fermentation Method with Agro-Industrial Residues as Renewable Feedstocks

Cunha

Vasconcellos

Florencio

et al. 2016

Waste Biomass Valor

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Agro-industrial residues have great potential as low-cost feedstocks for production of hydrolytic enzymes, which play a key role in the economics of cellulosic ethanol production. The goal of this research was to add value to agro-industrial byproducts such as wheat bran (WB), soybean bran, and sugarcane bagasse [either untreated (SCB) or pretreated (SCB p )] by using these materials for on-site production of enzymes employing a non-conventional solid-state and submerged fermentation method. The results were compared to conventional submerged fermentation, and three enzymatic cocktails were selected for use in SCB p hydrolysis. The non-conventional method was successfully validated for enzyme production using all the feedstocks. Compositional analysis and FTIR spectra showed the advantages of using WB, rather than SCB, due to its lower C:N ratio and lower contents of lignin and phenolic derivatives. The use of a WB:SCB mixture resulted in endoglucanase production that was up to 91 % higher than achieved using SCB alone. The best hydrolysis performance was achieved with the WB enzymatic cocktail, and the mixture of WB:SCB p resulted in cocktails with performance 51 % higher than cocktails produced with SCB p alone. The non-conventional on-site enzyme production using agro-industrial byproducts could potentially contribute to the implementation of large-scale 2G ethanol plants.

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Section: Resultsmentioning

confidence: 98%

Section: Agro-industrial Residues Characterizationmentioning

confidence: 99%

On-Site Production of Enzymatic Cocktails Using a Non-conventional Fermentation Method with Agro-Industrial Residues as Renewable Feedstocks

Cunha

Vasconcellos

Florencio

et al. 2016

Waste Biomass Valor

View full text Add to dashboard Cite

show abstract

“…Thus, recent studies have focused on the improvement of the fermentation processes for microbial enzyme production, as well as in the development of hyper-producing microbial strains, in order to reduce the costs and make the process economically viable [3][4][5]. Other possibility is the use of lignocellulosic biomass as a inducer source for the production of enzymes.…”

Section: Introductionmentioning

confidence: 99%

Production of Biomass-Degrading Enzymes by Trichoderma reesei Using Liquid Hot Water-Pretreated Corncob in Different Conditions of Oxygen Transfer

et al. 2019

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Enzymatic hydrolysis accounts for 20% of the total cost in the conversion process of lignocellulosic biomass into bioethanol. Therefore, production of biomass-degrading enzymes by using lignocellulosic residue as a fermentation substrate may be an alternative to decrease the production costs. In this study, corncob (CC) has been pretreated by liquid hot water (LHW) at 200°C for 30 min and used as inducer source for production of biomass-degrading enzymes by Trichoderma reesei MUM 97.53. The pretreatment was used to increase the cellulose content and the accessibility to lignocellulosic material. Although the filamentous fungus secreted a broad range of cellulolytic and hemicellulolytic enzymes when grown on untreated CC, higher enzyme productions were obtained when cultured on LHW-pretreated CC in a 2-L stirred tank bioreactor (STB). Besides, the effects of aeration (2 and 4 vvm) and agitation (150 and 250 rpm) rates on enzyme production were studied by submerged fermentation in a batch STB and correlated with the volumetric oxygen transfer coefficient (k L a). Maximal cellulase, xylanase, and βxylosidase productions were found at 150 rpm and 4 vvm, while the highest β-glucosidase levels were obtained at 150 rpm and 2 vvm, that corresponded to k L a values of 32.50 h −1 and 16.41 h −1 , respectively. At higher agitation, a lower enzymatic production was observed probably due to the high shear stress in the fungal hyphae.

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“…5,31 Ex situ methods have shown the inverse correlation between viscosity and shear rate; however, these observations were based on trials using high rotational speeds. 32,33 To promote mixing at lower rotational speeds, the diameter of the upper axial-flow impeller was increased in trial 5 (from 6 in to 8 in OD), increasing the shear rate. The number of fiber additions was also increased (from 10 to 14) during the first 24 h of hydrolysis.…”

Section: Impact Of Moderate Rotational Speeds On Mixing Of the Slurrymentioning

confidence: 99%

The Effect of Fed-Batch Operation and Rotational Speed on High-Solids Enzymatic Hydrolysis of Hardwood Substrates

GaonaAdriana

LawryshynYuri

SavilleBradley

2015

Industrial Biotechnology

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Enzymatic hydrolysis of hardwood substrates yields soluble sugars, which are a carbon source for producing ethanol and butanol. However, as the substrate and water are mixed, a viscous, heterogeneous slurry forms. The result is reduced mixing performance, mass transfer, and enzyme-substrate contact, all of which negatively affect sugar titers. In this study, enzymatic hydrolysis of a hardwood substrate at 20 wt% was conducted in a 10L stirred tank reactor. We investigated the dynamic changes in slurry behavior, as well as the interactions among mixer design/ operation and fed-batch strategies. The effects of the number and frequency of substrate additions, impeller configuration, and high and moderate agitation speeds were evaluated using torque measurements and in terms of glucan-to-glucose conversion. Fewer additions corresponds to batch operation and negatively impacted solids distribution, accurate torque readings, and glucan conversion. However, as the number of additions incrementally increased, glucan conversion increased, torque readings captured the resistance of the fluid to flow, and the solids distribution within the reactor improved. Moderate agitation speeds of 60 revolutions per minute (rpm), combined with smaller and more frequent fiber additions, led to increased glucan conversion and less torque resistance compared to similar experiments conducted at high rotational speeds (150 rpm), and the use of different impeller sizes and configurations impacted the distribution of the slurry. This work illustrates that the implementation of different fed-batch additions coupled with different impeller types and configurations promotes hardwood slurry blending, accommodates the increase in viscosity, and enables quantification of energy and power consumption.

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Liquefaction of sugarcane bagasse for enzyme production

Cited by 34 publications

References 17 publications

On-Site Production of Enzymatic Cocktails Using a Non-conventional Fermentation Method with Agro-Industrial Residues as Renewable Feedstocks

On-Site Production of Enzymatic Cocktails Using a Non-conventional Fermentation Method with Agro-Industrial Residues as Renewable Feedstocks

Production of Biomass-Degrading Enzymes by Trichoderma reesei Using Liquid Hot Water-Pretreated Corncob in Different Conditions of Oxygen Transfer

The Effect of Fed-Batch Operation and Rotational Speed on High-Solids Enzymatic Hydrolysis of Hardwood Substrates

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